A5046473364- Trace Elements in Health
- Lymphatic System and Diseases
- Iron Metabolism and Disorders
- Cancer Cells and Metastasis
- Drug-Induced Hepatotoxicity and Protection
- Silymarin and Mushroom Poisoning
- Atherosclerosis and Cardiovascular Diseases
- Heavy Metal Exposure and Toxicity
- Adipose Tissue and Metabolism
- Peptidase Inhibition and Analysis
- Cutaneous lymphoproliferative disorders research
- RNA modifications and cancer
- Renin-Angiotensin System Studies
- Drug Transport and Resistance Mechanisms
- Thermoregulation and physiological responses
- Circadian rhythm and melatonin
- Adipokines, Inflammation, and Metabolic Diseases
- Sirtuins and Resveratrol in Medicine
- RNA Research and Splicing
- Lymphatic Disorders and Treatments
- Plant Toxicity and Pharmacological Properties
- Nitric Oxide and Endothelin Effects
- Thyroid Cancer Diagnosis and Treatment
- Diet and metabolism studies
- Cardiac Fibrosis and Remodeling
Wroclaw Medical University
2016-2025
Morpho (United States)
2025
Wroclaw Research Centre EIT+ (Poland)
2020
Łukasiewicz Research Network – PORT Polish Center for Technology Development
2013-2014
University of Wrocław
2006-2013
Pula B, Jethon A, Piotrowska Gomulkiewicz Owczarek T, Calik J, Wojnar Witkiewicz W, Rys Ugorski M, Dziegiel P & Podhorska-Okolow M (2011) Histopathology 59, 1249–1260 Podoplanin expression by cancer-associated fibroblasts predicts poor outcome in invasive ductal breast carcinoma Aims: It has recently been shown that podoplanin, a mucin-type glycoprotein, is expressed cancer cells and (CAFs), promotes cell migration invasiveness. The biological role of podoplanin tumour stroma the (IDC) not...
The aim of the study was to evaluate localization and intensity RNA-binding motif single-stranded-interacting protein 3 (RBMS3) expression in clinical material using immunohistochemical (IHC) reactions cases ductal breast cancer (in vivo), determine level RBMS3 at both mRNA levels cell lines vitro). Moreover, data obtained vivo vitro studies were correlated with clinicopathological profiles patients. Material for IHC comprised 490 invasive carcinoma (IDC) 26 mastopathy tissues. Western blot...
With their ability to cross the blood-brain barrier, transport and regulate release of various signaling molecules, extracellular vesicles (EVs) constitute a novel avenue study intercellular communication in pathologies, including psychiatric disorders. We studied 34 major depressive disorder (MDD) patients 57 healthy controls characterized EVs isolated from plasma using digital holographic tomography (DHT) nanoparticle tracking analysis (NTA). detected DHT were markedly smaller less...
Abstract Background Extracellular vesicles (EVs) play a crucial role in facilitating intercellular communication. Their capacity to traverse the blood-brain barrier, transport, and control release of diverse neurotransmitters underscores their potential involvement pathophysiology psychiatric disorders (1). The small size biogenesis EVs complicates detection investigation (2). However, exosomal markers, including CD63 CD9, may provide convenient method for major depressive disorder (MDD)....
It was shown recently on the level of gene expression that UGT8, coding UDP-galactose:ceramide galactosyltransferase, is one six genes whose elevated correlated with a significantly increased risk lung metastases in breast cancer patients. In this study primary tumours and their as well cell lines were analysed for UGT8 at protein level.Expression tissue specimens using IHC, real-time PCR Western blotting.Comparison average values reaction intensities (IRS scale) showed significant...
Background: The microenvironment of solid tumours is significant in cancer development and progression. aim this study was to determine periostin (POSTN) expression by cancer-associated fibroblasts (CAFs) non-small-cell lung (NSCLC), as well assess associations with clinicopathological factors prognosis. Materials Methods: Immunohistochemical analysis POSTN performed on NSCLC (N = 700) non-malignant tissue (NMLT) 110) using microarrays. Laser capture microdissection (LCM) for isolation...
Amatoxin poisoning is caused by mushroom species belonging to the genera Amanita, Galerina and Lepiota with majority of lethal exposures attributable Amanita phalloides. High mortality rate in intoxications these mushrooms principally a result acute liver failure following significant hepatocyte damage due hepatocellular uptake amatoxins. A wide variety amatoxins have been isolated; however, alpha-amanitin (alpha-AMA) appears be primary toxin. Studies vitro vivo suggest that alpha-AMA does...
It has been recently found that metallothionein-3 (MT3) enhances the invasiveness and tumorigenesis of prostate cancer cells. This finding is in contrast to those earlier studies, which indicated overexpression MT3 breast cell lines inhibits their growth vitro. Therefore, clarify role progression, we analyzed effect MT3-overexpression on proliferation, invasiveness, migration, MDA-MB-231/BO2 was cells overexpressing were characterized by increased vitro, compared control Interestingly, this...
Background: Recent in vitro studies have indicated that irisin inhibits proliferation, migration and epithelial-mesenchymal transition. Irisin expression has not been studied tumour tissues of non-small cell lung cancer (NSCLC) patients yet. The aim the study was to determine NSCLCs comparison clinicopathological factors TTF-1, p63 Ki-67. Material methods: Tissue microarrays with 729 NSCLC 140 non-malignant tissue (NMLT) were used perform immunohistochemical reactions. Laser Capture...
Cornelian cherry (Cornus mas L.) fruits possess potential cardiovascular, lipid-lowering and hypoglycemic bioactivities. The aim of this study is to evaluate the influence resin-purified cornelian extract rich in iridoids anthocyanins on several transcription factors, intima/media ratio aorta serum parameters, which determine or are valuable indicators adverse changes observed course atherosclerosis, cardiovascular disease, metabolic syndrome. For purpose, male New Zealand rabbits were fed a...
Atherogenesis leads to the development of atherosclerosis, a progressive chronic disease characterized by subendothelial lipoprotein retention and endothelial impairment in arterial wall. It develops mainly as result inflammation also many other complex processes, which arise from, among others, oxidation adhesion. Cornelian cherry (Cornus mas L.) fruits are abundant iridoids anthocyanins-compounds with potent antioxidant anti-inflammatory activity. This study aimed determine effect two...
Our study aimed at examining significance of metallothionein (MT) expression in ductal breast cancers by determination a relationship between MT protein (MT-1/2) and selected prognostic factors, including grade histological differentiation (G), Ki-67 proliferative antigen, estrogen receptors (ER) progesterone (PgR) HER-2 receptor. Material for the studies involved 54 samples invasive cancer, manifesting malignancy grades G1-G3. In paraffin sections examined tumours immunohistochemical...
Multi-drug resistance (MDR) is the main cause of low effectiveness cancer chemotherapy. P-glycoprotein (P-gp) one factors determining MDR. Some studies indicate potential role melatonin (MLT) in In this study, we examined effect MLT on colon cell’s to doxorubicin (DOX). Using sulforhodamine B (SRB), method tested substances survival LoVo (colon cells sensitive DOX) and LoVoDX resistant was rated. immunocytochemistry (ICC), expression P-gp determined. With real-time PCR (RT-PCR) technique,...
α-Amanitin (α-AMA) is the main toxin of Amanita phalloides and its subspecies (A. virosa A. verna). The primary mechanism α-AMA toxicity associated with protein synthesis blocking in hepatocytes. Additionally, exhibits prooxidant properties that may contribute to severe hepatotoxicity. aim present study was assess effect on lipid peroxidation activities superoxide dismutase (SOD) catalase (CAT) human hepatocyte culture. effects benzylpenicillin (BPCN), N-acetyl-L-cysteine (ACC), silibinin...
Multidrug resistance (MDR) is a notable problem in the use of chemotherapy. Therefore, studies aimed at identifying substances capable overcoming cancer cells are required. Examples these compounds polyphenols, including resveratrol, that exert range various biological activities. The aim present study was to demonstrate effect 3,5,4'‑trihydroxy‑trans‑stilbene (resveratrol) on expression ATP binding cassette subfamily B member 1, Annexin A1 (ANXA1) and thioredoxin (TXN) genes, proteins...
Successful long-term kidney allograft survival with parallel reduction of complications resulting from prolonged immunosuppressive treatment is a goal in transplantation. We studied the immune changes cell phenotypes and gene expression induced by Our was to find phenotypic and/or transcriptional pattern that might be considered prognostic for transplant outcome. The analysis performed prospectively on 36 KTx recipients sampled during first year followed five years after transplantation 40...