A5006201564- Barrier Structure and Function Studies
- Drug Transport and Resistance Mechanisms
- Alkaloids: synthesis and pharmacology
- Neurological Disease Mechanisms and Treatments
- Neuroscience and Neuropharmacology Research
- Neuroinflammation and Neurodegeneration Mechanisms
- Medicinal Plants and Neuroprotection
- Cholinesterase and Neurodegenerative Diseases
- Synthesis of Organic Compounds
- Pharmacological Effects and Toxicity Studies
- Viral Infections and Immunology Research
- RNA and protein synthesis mechanisms
- Alzheimer's disease research and treatments
- Chemical synthesis and alkaloids
- Traditional and Medicinal Uses of Annonaceae
- RNA regulation and disease
- Caveolin-1 and cellular processes
- Biological Stains and Phytochemicals
- Pharmacology and Nanomedicine Research
- Intracerebral and Subarachnoid Hemorrhage Research
- Natural Compound Pharmacology Studies
- Ginger and Zingiberaceae research
- Molecular Sensors and Ion Detection
- RNA Interference and Gene Delivery
- Child Development and Digital Technology
Universiti Sains Malaysia
2014-2024
Hospital Universiti Sains Malaysia
2013-2018
King's College London
2009-2017
Since ancient times, essential oils (EOs) derived from aromatic plants have played a significant role in promoting human health. EOs are widely used biomedical applications due to their medicinal properties. and constituents been extensively studied for treating various health-related disorders, including cancer. Nonetheless, limited several drawbacks. Recent advances nanotechnology offer the potential utilising EO-loaded nanoparticles treatment of diseases. In this aspect, chitosan (CS)...
Abstract Mitragyna speciosa is reported to be beneficial for the management of chronic pain and opioid withdrawal in evolving epidemic. Data on blood–brain barrier (BBB) transport mitragynine 7‐hydroxymitragynine, active compounds plant, are still lacking inconclusive. Here, we present first time rate extent 7‐hydroxymitragynine across BBB, with an investigation their post‐BBB intra‐brain distribution. We utilized vitro BBB model study permeation interaction efflux transporter P‐glycoprotein...
Enterovirus A71 (EV-A71) causes hand, foot and mouth disease epidemics with neurological complications fatalities. However, the neuropathogenesis of EV-A71 remains poorly understood. In mice, adaptation virulence determinants have been mapped to mutations at VP2-149, VP1-145 VP1-244. We investigate how these amino acids alter heparin-binding phenotype shapes in one-day old mice. constructed six viruses varying residues VP1-98, (which are both determinants) VP2-149 (based on wild type...
30% of epilepsy patients receiving antiepileptic drugs (AEDs) are not fully controlled by therapy. The drug transporter hypothesis for refractory proposes that P-gp is over expressed at the epileptic focus with a role in extruding AEDs from brain. However, there controversy regarding whether all substrates this transporter. Our aim was to investigate transport phenytoin, lamotrigine and carbamazepine using seven in-vitro models. Uptake assays CEM/VBL cell lines, oocytes expressing human an...
Centella asiatica (CA) is a widely used traditional herb, notably for its cognitive enhancing effect and potential to increase synaptogenesis. The α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptors (AMPARs) N-methyl-D-aspartate (NMDARs) mediate fast excitatory neurotransmission with key roles in long-term potentiation which believed be the cellular mechanism of learning memory. Improved memory can an indication surface expression level these receptors. Our previous study...
Abstract Introduction Centella asiatica is an herbal plant that contains phytochemicals are widely believed to have positive effects on cognitive function. The adolescent stage a critical development period for the maturation of brain processes encompass changes in physical and psychological systems. However, effect C. has not been extensively studied adolescents. aim this study was therefore investigate extract enhancement learning memory rats. Methods locomotor activity, learning, were...
Embelin is well-known in ethnomedicine and reported to have central nervous system activities. However, there no report on blood-brain barrier (BBB) permeability of embelin. Here the BBB embelin was evaluated using vitro primary porcine brain endothelial cell (PBEC) model BBB. also for acetylcholinesterase (AChE) inhibitory activity docking prediction interaction with AChE amyloid beta (Aβ) binding sites. found be non-toxic PBECs did not disturb PBEC function. The showed restrictive tight...
The aim of this protocol presents an optimized procedure for the purification and cultivation pBECs to establish in vitro blood-brain barrier (BBB) models based on mono-culture (MC), MC with astrocyte-conditioned medium (ACM), non-contact co-culture (NCC) astrocytes porcine or rat origin. were isolated cultured from fragments capillaries brain cortices domestic pigs 5-6 months old. These purified by careful removal meninges, isolation homogenization grey matter, filtration, enzymatic...
In vitro blood-brain barrier (BBB) models from primary brain endothelial cells can closely resemble the in vivo BBB, offering valuable to assay BBB functions and screen potential central nervous system drugs. We have recently developed an model using porcine cells. The shows expression of tight junction proteins high transendothelial electrical resistance, evidence for a restrictive paracellular pathway. Validation studies small drug-like compounds demonstrated functional uptake efflux...
Abstract α‐Mangostin has been reported to possess a broad range of pharmacological effects including potent cholinesterase inhibition, but the development α‐mangostin as potential lead compound is impeded by its toxicity. The present study investigated impact simple structural modification on inhibitory activities and toxicity toward neuroblastoma liver cancer cells. dialkylated derivatives retained good acetylcholinesterase (AChE) with IC 50 values between 4.15 6.73 µM, not...
Abstract Enterovirus A71 (EV-A71) causes hand, foot and mouth disease epidemics with neurological complications fatalities. However, the neuropathogenesis of EV-A71 remains poorly understood. In mice, adaptation virulence determinants have been mapped to mutations at VP1-145, VP1-244 VP2-149. We hypothesized that heparin-binding phenotype shapes in mice. constructed six viruses varying residues VP1-98, VP1-145 (which are both determinants) VP2-149 (based on wild type 98E/145Q/149K, termed...
The aim of this protocol presents an optimized procedure for the purification and cultivation pBECs to establish in vitro blood-brain barrier (BBB) models based on mono-culture (MC), MC with astrocyte-conditioned medium (ACM), non-contact co-culture (NCC) astrocytes porcine or rat origin. were isolated cultured from fragments capillaries brain cortices domestic pigs 5-6 months old. These purified by careful removal meninges, isolation homogenization grey matter, filtration, enzymatic...
Euphorbia hirta is a plant belonging to the Euphorbiaceae family that widely used in traditional medicine treat various dermal diseases tropical and subtropical countries. It has been found have anti-inflammatory wound-healing effects, which may make it promising natural treatment for different medical conditions. The aim of present study focuses on investigation main active compounds associated with activity plants. powder aerial part was sequentially extracted n-hexane, chloroform,...