A5008573356- Photodynamic Therapy Research Studies
- Dental Implant Techniques and Outcomes
- Bone Tissue Engineering Materials
- Nanoplatforms for cancer theranostics
- Laser Applications in Dentistry and Medicine
- Dental materials and restorations
- Antifungal resistance and susceptibility
- Periodontal Regeneration and Treatments
- Bacterial biofilms and quorum sensing
- Endodontics and Root Canal Treatments
- Thyroid Cancer Diagnosis and Treatment
- Bone and Dental Protein Studies
- Antimicrobial Peptides and Activities
- Sirtuins and Resveratrol in Medicine
- Oral microbiology and periodontitis research
- Orthopaedic implants and arthroplasty
- Blood transfusion and management
- Dental Research and COVID-19
- Analytical Chemistry and Chromatography
- Liver physiology and pathology
- Collagen: Extraction and Characterization
- Cardiomyopathy and Myosin Studies
- Dental Trauma and Treatments
- Wnt/β-catenin signaling in development and cancer
- Medical and Biological Ozone Research
University of Chieti-Pescara
1991-2025
The use of biomaterial for tissue repair involves the interaction between materials and cells, coagulum formation represents first step healing. This process is particularly critical in oral cavity, where wounds are immediately subjected to masticatory mechanical stress, saliva invasion, bacterial attack. Therefore, present study aimed explore structural features biological activities a hemostatic collagen sponge on human gingival fibroblasts (HGFs) osteoblasts (HOBs). microstructure was...
Current research on dental implants has mainly focused the influence of surface roughness rate osseointegration, while studies development surfaces to also improve interaction peri-implant soft tissues are lacking. To this end, first purpose study was evaluate response human gingival fibroblasts (hGDFs) titanium implant discs (Implacil De Bortoli, Brazil) having different micro and nano-topography: machined (Ti-M) versus sandblasted/double-etched (Ti-S). The secondary aim investigate effect...
Colorectal cancer (CRC) is a multistep process that arises in the colic tissue microenvironment. Oxidative stress plays role mediating CRC cell survival and progression, as well promoting resistance to therapies. progression associated with Wnt/β-Catenin signaling dysregulation loss of proper APC functions. Cancer recurrence/relapse has been attributed altered ROS levels, produced cancerous The effect oxidative distress on light functions unclear. This study evaluated H2O2-induced short-term...
The first aim of this study was to characterize the surface topography a novel 3D-printed dental implant at micro- and macro-level. Its second evaluate osteogenic, angiogenic, immunogenic responses human oral osteoblasts (hOBs), gingival fibroblasts (hGFs), mesenchymal stem cells (hAD-MSCs), monocytes surface.
In the oil sector, a novelty in centrifugal extraction system is represented by multi-phase decanters (DMF) that work without adding process water and with advantage of recovering dried pomace by-product, called “pâté”, consisting pulp its vegetation water, traces stone. The pâté has high content phenolic compounds, mainly secoiridoids verbascoside. present investigated efficacy two different ways debittering (by sequential filtrations spontaneous fermentation) DMF from three olive cultivars...
The use of collagen membranes has remained the gold standard in GTR/GBR. In this study, features and biological activities an acellular porcine dermis matrix membrane applicable during dental surgery were investigated, also by applying hydration with NaCl. Thus, two tested distinguished, H-Membrane Membrane, compared to control cell culture plastic. characterization was performed SEM histological analyses. contrast, biocompatibility investigated on HGF HOB cells at 3, 7, 14 days MTT for...
The study of the organizational kinetics in area surrounding transmucosal part dental implants promises to ensure an accurate diagnosis healing process, terms osseointegration and long-term implant success. In this demonstrative work, morphological, qualitative quantitative characteristics 3D images collagen bundles obtained by synchrotron-based high-resolution X-ray tomography were analyzed. Data analysis was performed using deep learning algorithms, neural networks that applied on multiple...
This study aimed to evaluate the ability of photodynamic therapy, based on use a gel containing 5% delta aminolaevulinic acid (ALAD) for 45′ followed by irradiation with 630 nm LED (PDT) 7′, eradicate Candida albicans strains without damaging gingiva. C. oral and gingival fibroblasts (hGFs) were used achieve these goals. The potential antifungal effects clinical resistant S5 strain evaluated in terms biofilm biomass, colony forming units (CFU/mL) count, cell viability live/dead analysis,...
Background: In recent years, the use of conometric systems to connect dental implant abutments and prosthetic caps has been advocated because they seem eliminate side effects reported when using screw- cement-connected restorations. Objectives: The present case study is focused on connection characterization its performance in terms microarchitecture peri-implant soft tissues by a cross-linked approach based optical microscopy three-dimensional imaging. Methods: Two implants were...
Thyroid diseases have a complex and multifactorial aetiology. Despite the numerous studies on signals referable to malignant transition, molecular mechanisms concerning role of oxidative stress remain elusive. Based its strong power, H2O2 could be responsible for high level DNA damage observed in cancerous thyroid tissue hyperactivation mitogen-activated protein kinase (MAPK) PI3K/Akt, which mediate ErbB signaling. Increased levels 8-oxoG adducts been detected early stages cancer. These...
Aim of the study: The aim was to evaluate effects two LED devices, TL-01 and TL-03 in photodynamic therapy (PDT), on Enterococcus faecalis human gingival fibroblasts (HGFs). TL-01, characterized by a single emitter, irradiates one periodontal site at time, whereas multi-led device (TL-03) all vestibular sites arch time. Methods: E. bacterial suspensions HGFs were incubated for 45 min with Aladent gel (ALAD) containing 5-aminolevulinic acid then exposed devices (ALAD-PDT), having different...
Implant surfaces are known to influence the osseointegration process; therefore, their modifications represent an important subject of investigation. On this basis, purpose study was evaluate response human oral osteoblasts (hOBs) three different GR4 titanium discs: Machined, double-etched (Osteopore), and double-etched, surface-enriched with calcium phosphorus (CaP) (Nanopore). The superficial topography investigated scanning electron microscopy (SEM) sessile drop technique. To test...
Apigenin (APG), a natural flavonoid compound with anti-inflammatory and antioxidative properties, was found to promote in vitro osteogenic differentiation accelerate vivo bone formation, indicating APG as promising molecule repair, potential clinical application bone-deficient conditions. In particular, dentistry, it is fundamental increase the available volume for implant placement maxilla. Therefore, this study aims investigate effects on osteoblasts (hOBs) obtained from human jaw. hOBs...
Background: In this ex vivo study, the aim was to evaluate effects of ALAD and red light on Enterococcus faecalis in infected root canals using a special intracanal fiber. Methods: A total 70 extracted, single-rooted teeth were used. The decoronated at length roots approximately 15 mm then instrumented. apical foramen sealed by composite resin, with pure culture E. ATCC 29212 for eight days 37 °C. Following contamination period, divided into seven groups, including positive negative control...
This study aimed to evaluate the effects of a new photodynamic protocol (ALAD-PDT), consisting 5% 5-aminolevulinic acid-gel and 630 nm-LED, already used for antibacterial in treatment periodontitis, on human gingival fibroblasts (HGF) primary osteoblasts (HOB). HGF HOB were incubated with different ALAD concentrations 45 min, subsequently irradiated nm-LED 7 min. Firstly, cytotoxicity at 24 h proliferation 48 72 assessed. Then intracellular content protoporphyrin IX (PpIX) ROS superoxide...
This study aims to test a photodynamic protocol based on gel containing aminolevulinic acid followed by red-LED (ALAD-PDT) irradiation human gingival fibroblasts (hGFs) and osteoblasts (hOBs) cultured porcine acellular dermal matrix membrane (PADMM). In the previous literature, ALAD-PDT showed solid antibacterial activity proliferative induction HGFs plates HOBs cortical lamina. PADMMs are used in dentistry periodontology treat recessions increase tissue thickness case of thin biotype...
This study evaluates the effects of different programs complex electromagnetic fields (C.M.F.s) on Candida albicans, in planktonic and sessile phase human gingival fibroblasts (HGF cells). In vitro cultures C. albicans ATCC 10231 HGF cells were exposed to cycles C.M.F.s defined as: oxidative stress, stress/antibacterial, antibacterial, antibacterial/oxidative stress. Colony forming units (CFUs), metabolic activity, viability (live/dead), cell morphology, filamentation analysis, cytotoxicity...
Recently, there has been an increasing interest in finding new approaches to manage oral wound healing. Although resveratrol (RSV) exhibited many biological properties, such as antioxidant and anti-inflammatory activities, its use a drug is limited by unfavorable bioavailability. This study aimed investigate series of RSV derivatives (1a-j) with better pharmacokinetic profiles. At first, their cytocompatibility at different concentrations was tested on gingival fibroblasts (HGFs). Among...
Guided bone regeneration (GBR) comprehends the application of membranes to drive healing and exclude non-osteogenic tissues from interfering with regeneration. However, may be exposed bacterial attack, risk failure GBR. Recently, an antibacterial photodynamic protocol (ALAD-PDT) based on a gel 5% 5-aminolevulinic acid incubated for 45 min irradiated 7 by LED light at 630 nm, also showed pro-proliferative effect human fibroblasts osteoblasts. The present study hypothesized that...
This study aimed to evaluate the effects of a new photodynamic protocol (ALAD-PDT) on primary human osteoblasts (hOBs). The ALAD-PDT consists heat-sensitive gel with 5% 5-delta aminolevulinic acid commercialized as Aladent (ALAD), combined 630 nm LED. For this purpose, hOBs, explanted from mandible bone fragments, were used and treated different ALAD concentrations (10%, 50%, 100% v/v) incubated for 45 min immediately afterwards irradiated LED device 7 min. untreated unirradiated cells...