A5045894601- Thyroid Cancer Diagnosis and Treatment
- Plasma Applications and Diagnostics
- Hernia repair and management
- TGF-β signaling in diseases
- Helicobacter pylori-related gastroenterology studies
- Intestinal and Peritoneal Adhesions
- Growth Hormone and Insulin-like Growth Factors
- Abdominal Surgery and Complications
- Advanced Proteomics Techniques and Applications
- Cancer, Hypoxia, and Metabolism
- Nanoplatforms for cancer theranostics
- Microfluidic and Capillary Electrophoresis Applications
- Genomics, phytochemicals, and oxidative stress
- HIV/AIDS oral health manifestations
- Gut microbiota and health
- Mass Spectrometry Techniques and Applications
- Immune cells in cancer
- Phytochemistry and Bioactive Compounds
- Innovative Microfluidic and Catalytic Techniques Innovation
- Adrenal and Paraganglionic Tumors
- Liver Disease Diagnosis and Treatment
- Heme Oxygenase-1 and Carbon Monoxide
- Oral microbiology and periodontitis research
- PI3K/AKT/mTOR signaling in cancer
- Impact of AI and Big Data on Business and Society
University of Chieti-Pescara
2014-2025
Ospedale Regionale di Bellinzona e Valli
2020-2023
Bridge University
2023
Kafrelsheikh University
2023
Kwangwoon University
2023
Ente Ospedaliero Cantonale
2022
Centre Hospitalier Universitaire de Nice
2019-2021
Institute for Research in Immunology and Cancer
2007-2008
Université de Montréal
2007-2008
Polo d’Innovazione di Genomica
2008
Helicobacter pylori persistence is associated with its capacity to develop biofilms as a response changing environmental conditions and stress. Extracellular DNA (eDNA) component of H. biofilm matrix but the lack DNase I activity supports hypothesis that eDNA might be protected by other extracellular polymeric substances (EPS) and/or Outer Membrane Vesicles (OMVs), which bleb from bacteria surface during growth. The aim present study was both identify presence on OMVs segregated ATCC...
Membrane vesicles (MVs) are bilayer structures which bleb from bacteria, and important in trafficking biomolecules to other bacteria or host cells. There few data about MVs produced by the Gram-positive commensal – derived probiotic Lactobacillus reuteri; however, this species may have potential therapeutic benefit. The aim of study was detect characterize biofilm (bMVs), planktonic (pMVs) phenotypes L. reuteri DSM 17938. were analyzed for structure physicochemical characterization Scanning...
Abstract Background Vaccinium corymbosum berries represent a source of anthocyanins and polyphenols studied tested for their healthy potential. The present study was aimed at isolating, characterizing, quantifying the anthocyanin‐enriched fraction obtained from blueberry assessing its biological protective effects against head neck cell lines under inflammatory‐related conditions. V. extract subjected to colorimetric analysis, antioxidant evaluation, HPLC‐DAD analysis. This characterized by...
Protein phosphorylation is a central cell signaling event that underlies broad spectrum of key physiological processes. Advances in affinity chromatography and mass spectrometry are now providing the ability to identify quantitate thousands sites simultaneously. Comprehensive phosphoproteome analyses present sizable analytical challenges view suppression effects phosphopeptides variable quality MS/MS spectra. This work presents an integrated enzymatic data mining approach enabling...
Oxidative stress is widely accepted as a key factor of doxorubicin (Doxo)‑induced cardiotoxicity. There evidence to indicate that nitrosative involved in this process, and Doxo interacts by amplifying cell damage. Mitochondrial connexin 43 (mitoCx43) can confer cardioprotective effects through the reduction mitochondrial reactive oxygen species production during Doxo‑induced The present study aimed evaluate involvement mitoCx43 stress. Rat H9c2 cardiomyoblasts were treated with absence or...
This study reports on the synthesis, structural assessment, microbiological screening against several strains of H. pylori and antiproliferative activity human gastric adenocarcinoma (AGS) cells a large series carvacrol-based compounds. Structural analyses consisted elemental analysis, 1H/13C/19F NMR spectra crystallographic studies. The structure-activity relationships evidenced that among ether derivatives substitution with specific electron-withdrawing groups (CF3 NO2) especially in para...
Abstract The aim of this study was to evaluate the antimicrobial efficacy an air gas soft jet CAP for its potential use in removing oral biofilms, given that plasma-based technologies have emerged as promising methods periodontology. Two types biofilms were developed, one by Streptococcus mutans UA 159 bacterial strain and other a complex mixture saliva microorganisms isolated from patient with periodontitis. This latter biofilm characterized via Next Generation Sequencing determine main...
Abstract Objective Cold atmospheric plasma (CAP) is a novel approach for cancer treatment. It can be used to treat liquids—plasma‐activated media (PAM)—which are then transferred the target as an exogenous source of reactive oxygen and nitrogen species (RONS). The present study aimed at chemically characterizing different PAM assessing their in vitro selectivity against head neck cells (HNC). Methods were obtained by exposing 2 5 mL cell culture medium CAP 5, 10 20 min 6 mm working distance....
Normal epithelial thyroid cells in culture are inhibited by TGF-1. Instead, transformed cell lines frequently resistant to its growth inhibitory effect. Loss of TGF- responsiveness could be due a reduced expression receptors, as shown rat and human tumors, or alterations other genes controlling signal transduction pathway. However, neoplasia, complex pattern occurring during transformation progression has been identified. Functionally, TGF-1 acts tumor suppressor the early stage promoter...
Many clinical studies highlight the dichotomous role of PRDXs in human cancers, where they can exhibit strong tumor-suppressive or tumor-promoting functions. Recent evidence suggests that lower expression correlates with cancer progression colorectal (CRC) esophageal squamous carcinoma. In thyroid, increased levels PRDX1 has been described follicular adenomas and carcinomas, as well thyroiditis, while reduced PRDX6 found adenomas. We studied PRDX6, a series thyroid tissue samples, covering...
Head and neck paragangliomas (HNPGLs) are rare tumors that may cause important morbidity, because of their tendency to infiltrate the skull base. At present, surgery is only therapeutic option, but radical removal be difficult or impossible. Thus, effective targets molecules for HNPGL treatment need identified. However, lack cellular models this tumor hampers task. PPARα receptor activation was reported in several appears a promising target different malignancies. Considering role HNPGLs...
Colorectal cancer (CRC) is a multistep process that arises in the colic tissue microenvironment. Oxidative stress plays role mediating CRC cell survival and progression, as well promoting resistance to therapies. progression associated with Wnt/β-Catenin signaling dysregulation loss of proper APC functions. Cancer recurrence/relapse has been attributed altered ROS levels, produced cancerous The effect oxidative distress on light functions unclear. This study evaluated H2O2-induced short-term...
Following a similar approach on carvacrol-based derivatives, we investigated the synthesis and microbiological screening against eight strains of H. pylori, cytotoxic activity human gastric adenocarcinoma (AGS) cells new series ether compounds based structure thymol. Structural analysis comprehended elemental 1H/13C/19F NMR spectra. The structure–activity relationships within this molecular library 38 structurally-related reported that some chemical modifications OH group thymol led to...
Thyroid diseases have a complex and multifactorial aetiology. Despite the numerous studies on signals referable to malignant transition, molecular mechanisms concerning role of oxidative stress remain elusive. Based its strong power, H2O2 could be responsible for high level DNA damage observed in cancerous thyroid tissue hyperactivation mitogen-activated protein kinase (MAPK) PI3K/Akt, which mediate ErbB signaling. Increased levels 8-oxoG adducts been detected early stages cancer. These...
Despite advances in biological and molecular characteristics, the prognosis of oral squamous cell carcinomas is still very unfavourable based on classical clinicopathological parameters. However, tumors with similar characteristics may differ dramatically their clinical outcome. Thus, identification novel prognostic factors necessary to improve therapeutic approaches. Transforming growth factor-beta1 (TGF-beta1) a potent inhibitor epithelial proliferation, thus, inactivation TGF-beta1...
Sulfation and phosphorylation are post-translational modifications imparting an isobaric 80-Da addition on the side chain of serine, threonine, or tyrosine residues. These two often difficult to distinguish because their similar MS fragmentation patterns. Targeted identification these in specific proteins commonly relies prior separation using gel electrophoresis silver staining. In present investigation, we report a potential pitfall interpretation from silver-stained gels due artifactual...
Fractal dimension (FD) in tissue specimens from patients with oral squamous cell carcinoma (OSCC) was evaluated. FD values different stages of OSCC, and the correlations clinicopathological variables patient survival were investigated. Histological sections OSCC control non‐neoplastic mucosa stained hematoxylin–eosin for pathological analysis Feulgen nuclear evaluation. groups vs. controls revealed statistically significant differences ( P < 0.001). In addition, a progressive increase...
Abstract Background Flank hernias are uncommon, surgical treatment is challenging and the minimally-invasive approach not always feasible. The aim of this study was to report safety feasibility robotic-assisted repair. Methods approved by local ethic committee (2019–01132 CE3495). A retrospective search on a prospectively collected dataset including demographic clinical records robotic surgery at our institution performed identify patients treated for flank hernia. Patients were followed-up...
A monofunctional prephenate dehydrogenase (PD) from Aquifex aeolicus was expressed as a His-tagged protein in Escherichia coli and purified by nickel affinity chromatography allowing the first biochemical biophysical characterization of thermostable PD. A. PD is susceptible to proteolysis. In this report, properties full-length are compared with one these products, an N-terminally truncated variant (Delta19PD) also recombinantly E. coli. Both forms dimeric show maximum activity at 95 degrees...
Insulin activates several processes potentially dangerous for the arterial wall and hyperinsulinemia might be atherogenic. However, other insulin effects are protective vessel thus anti-atherogenic. Aim of this study was to investigate whether on pro-atherogenic anti-atherogenic were differently affected in cells from insulin-resistant individuals.We determined effect nitric oxide (NO) production plasminogen activator inhibitor (PAI)-1 synthesis 12 fibroblast strains obtained skin biopsy...