A5046381826- MicroRNA in disease regulation
- Amoebic Infections and Treatments
- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- Parasitic Infections and Diagnostics
- Circular RNAs in diseases
- RNA Research and Splicing
- Angiogenesis and VEGF in Cancer
- Cancer-related Molecular Pathways
- Cancer Cells and Metastasis
- Extracellular vesicles in disease
- Diagnosis and treatment of tuberculosis
- Metabolism, Diabetes, and Cancer
- Ubiquitin and proteasome pathways
- Neonatal Respiratory Health Research
- Mycobacterium research and diagnosis
- Parasites and Host Interactions
- Epigenetics and DNA Methylation
- Immune cells in cancer
- Sirtuins and Resveratrol in Medicine
- Immune responses and vaccinations
- Cancer, Hypoxia, and Metabolism
- Protein Tyrosine Phosphatases
- Histone Deacetylase Inhibitors Research
- Tuberculosis Research and Epidemiology
Universidad Nacional Autónoma de México
2014-2025
Universidad Autónoma de la Ciudad de México
2008-2024
Latent tuberculosis is characterized by the presence of dormant, nonreplicating (DNR) bacilli for years without causing clinical signs and symptoms, remaining as a major reservoir active tuberculosis. The mechanism through which M. transits from DNR to remains unclear. However, resuscitation-promoting factors (Rpfs) could participate in reactivation. Using recombinant bovis BCG that expresses rpfC (M. BCG-pMV261::rpfC), we evaluated role RpfC growth expression 11 hypoxia-regulated genes...
Tumors have high requirements in terms of nutrients and oxygen. Angiogenesis is the classical mechanism for vessel formation. Tumoral vascularization has function nourishing cancer cells to support tumor growth. Vasculogenic mimicry, a novel intratumoral microcirculation system, alludes ability organize three-dimensional (3D) channel-like architectures. It also supplies tumors with Both mechanisms operate coordinated way; however, their functions breast stem-like regulation by microRNAs...
Aberrant expression of microRNAs has been associated with migration tumor cells. In this study, we aimed to investigate the biological significance miR-944 whose function is unknown in breast cancer.MiR-944 cancer cells and tumors was evaluated by Taqman qRT-PCR assays. Transcriptional profiling MDA-MB-231 expressing performed using DNA microarrays. Cell viability, invasion were assessed MTT, scratch/wound-healing transwell chamber assays, respectively. The luciferase reporter assay used...
Vasculogenic mimicry (VM) is a novel cancer hallmark in which malignant cells develop matrix-associated 3D tubular networks with lumen under hypoxia to supply nutrients needed for tumor growth. Recent studies showed that microRNAs (miRNAs) may have role VM regulation. In this study, we examined the relevance of hypoxia-regulated miRNAs (hypoxamiRs) early stages formation. Data after 48 h and 12 incubation on matrigel SKOV3 ovarian undergo formation intercellular connections referred...
HOX transcript antisense RNA (HOTAIR) is an oncogenic long non-coding frequently overexpressed in cancer. HOTAIR can enhance the malignant behavior of tumors by sponging microRNAs with tumor suppressor functions. Vasculogenic mimicry a hypoxia-activated process which cells form three-dimensional (3D) channel-like networks, resembling endothelial blood vessels, to obtain nutrients. However, role vasculogenic and underlying mechanisms are unknown human cancers. In current study, we...
MicroRNAs (miRNAs) are small non-coding RNAs that function as negative regulators of gene expression. Recent evidences suggested host cells miRNAs involved in the progression infectious diseases, but its role amoebiasis remains largely unknown. Here, we reported an unexplored for human epithelial colon during apoptosis induced by Entamoeba histolytica. We demonstrated first time SW-480 change their profile response to parasite exposure. Our data showed virulent E. histolytica trophozoites...
Vasculogenic mimicry (VM) is characterized by formation of three-dimensional (3D) channels-like structures tumor cells, supplying the nutrients needed for growth. VM stimulated hypoxic microenvironment, and it has been associated with increased metastasis clinical poor outcome in cancer patients. cAMP responsive element (CRE)-binding protein 5 (CREB5) a hypoxia-activated transcription factor involved tumorigenesis. However, CREB5 functions if its regulated microRNAs remains unknown breast...
In higher eukaryotes, mRNA degradation and RNA-based gene silencing occur in cytoplasmic foci referred to as processing bodies (P-bodies). protozoan parasites, the presence of P-bodies their putative role decay have yet be comprehensively addressed. Identification might provide information on how machineries evolved lower eukaryotes. Here, we used immunofluorescence confocal microscopy assays investigate cellular localization proteins human intestinal parasite Entamoeba histolytica found...
Abstract Entamoeba histolytica is the intestinal parasite responsible for human amoebiasis that a leading cause of death in developing countries. In this protozoan, heterogeneity DNA content, polyploidy and genome plasticity have been associated to alterations mechanisms controlling replication cell division. Studying function transcription factor EhPC4, we unexpectedly found it functionally related multinucleation. Site-directed mutagenesis on FRFPKG motif revealed K 127 residue required...
Macrophages act as a reservoir for Mycobacterium tuberculosis, producing latent infection in approximately 90% of infected people. In this study, J774A.1 mouse macrophage cell line response and microRNA (miRNA) expression during with the most relevant mycobacterial strains humans (M. M. bovis BCG) was explored. No significant differences bacillary loads were observed between activate naive macrophages tuberculosis bovis. Nitrite production inhibition control accordance virulence strain....
Mycobacterium tuberculosis is a successful intracellular pathogen that uses multiple proteins to survive within macrophages, one of the most remarkable virulence factor EsxA. In this study, we evaluate participation EsxA in miRNAs expression profile human monocyte-derived macrophages (hMDM), mapping out contribution miRNA and how these changes can influence alter immune-related processes pathways. The cytotoxic effect rEsxA on hMDM was evaluated by neutral red assay. evaluation infected...
Nanodiamonds (ND-COOH) are used as drug delivery systems because of their attractive properties, they allow for optimized transport therapeutic agents in cellular models. Metformin (MET) is a diabetes mellitus therapy and exhibits anti-cancer properties. In this study, dispersed nanodiamonds were functionalized with metformin by directly binding them to 1,6-hexanediol (ND-MET), effects on the cytotoxicity breast ovarian cancer cells evaluated vitro. A simple synthesis ND-MET was performed...