A5102817071- Plant Molecular Biology Research
- Plant Reproductive Biology
- Photosynthetic Processes and Mechanisms
- Cancer-related molecular mechanisms research
- Soybean genetics and cultivation
- Legume Nitrogen Fixing Symbiosis
- RNA Research and Splicing
- Plant Gene Expression Analysis
- Plant Virus Research Studies
- Plant-Microbe Interactions and Immunity
- DNA and Nucleic Acid Chemistry
- Mycotoxins in Agriculture and Food
- Natural product bioactivities and synthesis
- NF-κB Signaling Pathways
- RNA modifications and cancer
- Plant and Fungal Interactions Research
- Cellular transport and secretion
- Immune Response and Inflammation
- RNA and protein synthesis mechanisms
- CRISPR and Genetic Engineering
- Genetic Mapping and Diversity in Plants and Animals
- Genetics and Plant Breeding
- Flavonoids in Medical Research
- Botanical Studies and Applications
- Essential Oils and Antimicrobial Activity
Nanchang University
2012-2024
The First People's Hospital of Changde
2024
Central South University
2024
United States Department of Agriculture
2003
Abstract Single-nucleotide polymorphisms (SNPs) provide an abundant source of DNA in a number eukaryotic species. Information on the frequency, nature, and distribution SNPs plant genomes is limited. Thus, our objectives were (1) to determine SNP frequency coding noncoding soybean (Glycine max L. Merr.) sequence amplified from genomic using PCR primers designed complete genes, cDNAs, random sequence; (2) characterize haplotype variation these sequences; (3) initial estimates linkage...
ABSTRACT Simple sequence repeat (SSR) genetic markers, also referred to as microsatellites, function in map‐based cloning and for marker‐assisted selection plant breeding. The objectives of this study were determine the abundance SSRs soybean genome develop test SSR markers create a database locus‐specific with high likelihood polymorphism. A total 210,990 di‐, tri‐, tetranucleotide repeats five or more identified whole (WGS) which included 61,458 consisting units di‐ (≥10), tri‐ (≥8), (≥7)....
Long noncoding RNAs (lncRNAs) are emerging as important regulators in plant development, but few of them have been functionally characterized fruit ripening. Here, we identified 25,613 lncRNAs from strawberry ripening fruits based on RNA-seq data poly(A)-depleted libraries and rRNA-depleted libraries, most which exhibited distinct temporal expression patterns. A novel lncRNA, FRILAIR harbours the miR397 binding site that is highly conserved diverse species. overexpression promoted maturation...
Abstract Background Cytoplasmic male sterility (CMS) has often been associated with abnormal mitochondrial open reading frames. The gene orfH79 is a candidate for causing the CMS trait in CMS-Honglian (CMS-HL) rice. However, whether expression can actually induce rice remains unclear. Results Western blot analysis revealed that ORFH79 protein mainly present mitochondria of CMS-HL and absent fertile line. To investigate function mitochondria, this was fused to transit peptide sequence used...
AIM: To investigate the protective effects, antioxidant potential, and anti-inflammatory mechanisms of eicosane on glutamate-induced cell damage N-methyl-D-aspartate (NMDA)-induced retinal ganglion (RGC) injury in a mouse model glaucoma. METHODS: The effects rat R28 precursor line were assessed using counting kit-8 assays Hoechst-propidium iodide staining. Intracellular reactive oxygen species (ROS) production was measured fluorescent probe 2'-7'-dichlorofluorescin diacetate flow cytometry....
Abstract Although many long noncoding RNAs have been discovered in plants, little is known about their biological function and mode of action. Here we show that the drought-induced intergenic RNA DANA1 interacts with L1p/L10e family member protein -INTERACTING PROTEIN 1 (DIP1) cell nucleus Arabidopsis, both DIP1 promote plant drought resistance. increase histone deacetylase HDA9 binding to CYP707A1 CYP707A2 loci. further PWWP3, a PEAT complex associates has activity. Mutation enhances...
High soils salinity is a main factor affecting agricultural production. Studying the function of salt-tolerance-related genes essential to enhance crop tolerance stress. Rab7 small GTP-binding protein that distributed widely among eukaryotes. Endocytic trafficking mediated by plays an important role in animal and yeast cells, but current understanding plants still very limited. Herein, we isolated vesicle gene, OsRab7 , from rice. Transgenic rice over-expressing exhibited enhanced seedling...
Salicylic acid (SA) is synthesized via the phenylalanine lyase (PAL) and isochorismate synthase (ICS) pathways can influence stress response in plants by regulating certain secondary metabolites. However, association between SA particular metabolites Chinese medicinal plant Scutellaria baicalensis Georgi unclear. To elucidate baicalin baicalein, which constitute primary effective components of S. baicalensis, we subjected seedlings to drought salt exogenous treatment a laboratory setting...
D1-cytoplasmic male sterility (CMS) rice is a sporophytic cytoplasmic male-sterile developed from Dongxiang wild that exhibits no-pollen-grain phenotype. A mitochondrial chimeric gene (orf182) was detected by genome sequencing and comparative analysis. Orf182 composed of three recombinant fragments, the largest which homologous to Sorghum bicolor sequences. In addition, orf182 found only in species collected China. Northern blot analysis showed transcripts were affected Rf genes...
Inhibitor of nuclear factor kappa-B kinase β (IKKβ) is a subunit the IKK complex. It can activate NF-κB pathway through phosphorylating IκB in response to wide range stimuli. In present study, an IKKβ gene from grass carp (Ctenopharyngodon idella; KT282114) was cloned and identified by homologous cloning rapid-amplification cDNA ends (RACE) technique. The complete CiIKKβ 3428 bp length, with longest open reading frame (ORF) 2337 encoding polypeptide 778 amino acids. deduced acid sequence has...
Cytoplasmic male sterility (CMS) has often been associated with abnormal mitochondrial open frames (ORF), orfH79 is a chimeric gene responsible for the CMS trait in Honglian (HL) rice. In this study, weakly produced ORFH79 protein significantly inhibited growth of E. coli an oxygen culture, however, transformants producing was indistinguishable from control under anaerobic incubation conditions. addition, lower respiration rate, wrinkled bacterial surfaces, and decreased pyruvate kinase...
PKZ (PKR-like) was discovered as a member of elF2α kinase family in fish, which possesses conserved catalytic domain an eIF2α C-terminal and also two Z-DNA-binding domains (Zα1 Zα2) N-terminal. can be activated through binding Zα to Z-DNA. However, the regulatory function still remains unclear. To investigate molecular mechanism how interacts with Z-DNA, we expressed polypeptide Zα1α2 Escherichia coli Rosetta strain purified by affinity chromatography on Ni-NTA resin. Different lengths...
PKZ, protein kinase containing Z-DNA domains, is a novel member of the vertebrate eIF2α family. Containing catalytic domain in C-terminus and two binding domains (Zα1 Zα2) N-terminus, PKZ can be activated through Zα to Z-DNA. However, regulatory function remains established. Here, understand impact on DNA conformational transition, wild-type Zα1Zα2 11 mutant proteins were expressed purified. At same time, several different lengths hairpins-d(GC)nT4(GC)n (n = 2-6) an RNA hairpin-r(GC)6T4(GC)6...