A5084492747- Nitric Oxide and Endothelin Effects
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Peptidase Inhibition and Analysis
- Signaling Pathways in Disease
- Cell Adhesion Molecules Research
- Molecular Biology Techniques and Applications
- Neuropeptides and Animal Physiology
- dental development and anomalies
- Angiogenesis and VEGF in Cancer
- Cancer, Hypoxia, and Metabolism
- Bone and Dental Protein Studies
- Oral and Maxillofacial Pathology
- Dental Implant Techniques and Outcomes
- Genetics and Physical Performance
- Protein Kinase Regulation and GTPase Signaling
- Adenosine and Purinergic Signaling
- Renin-Angiotensin System Studies
- Pain Mechanisms and Treatments
- Muscle Physiology and Disorders
- Endodontics and Root Canal Treatments
- Heme Oxygenase-1 and Carbon Monoxide
- Metabolism, Diabetes, and Cancer
- Aldose Reductase and Taurine
- Lymphatic System and Diseases
- Receptor Mechanisms and Signaling
University Medical Center of the Johannes Gutenberg University Mainz
2021-2024
Johannes Gutenberg University Mainz
2020-2024
University of Cologne
2004-2020
Institut für biologische Forschung
2019
University Hospital Cologne
2018
Sakarya University
2015
Heinrich Heine University Düsseldorf
2004-2013
German Sport University Cologne
2006-2008
Unfallkrankenhaus Berlin
2008
University Hospital Frankfurt
2008
While ryanodine receptor 1 (RyR1) critically contributes to skeletal muscle contraction abilities by mediating Ca²⁺ion oscillation between sarcoplasmatic and myofibrillar compartments, AMP-activated protein kinase (AMPK) senses contraction-induced energetic stress phosphorylation at Thr¹⁷². Phosphorylation of RyR1 serine²⁸⁴³ (pRyR1Ser²⁸⁴³) results in leaky channels impaired Ca²⁺homeostasis. Because acute resistance exercise exerts decreased performance muscle, preceded high rates...
Different studies have shown that HPV16‐positive OPSCC can be subdivided based on integration status (integrated, episomal and mixed forms). Because we showed neither affects the levels of viral genes, nor those virally disrupted human a genome‐wide screen was performed to identify genes which expression is influenced by clinical relevance. Thirty‐three fresh‐frozen HPV‐16 positive samples with known were analyzed mRNA profiling. Among interest, Aldo‐keto‐reductases 1C1 1C3 (AKR1C1, AKR1C3)...
The nitric oxide (NO) receptor enzyme soluble guanylate cyclase (sGC) contains one prosthetic heme group as an αβ heterodimer, and two heterodimer isoforms (α 1 β , α 2 ) were characterized to have activity. To test the irreversible inflammation-dependent regulation of sGC in odontoblasts, we incubated decalcified frozen sections healthy inflamed human third molars with antibodies against β-actin, nitrotyrosine, inducible synthase (iNOS), -, -subunits analyzed them at protein levels by...
The binding of nitric oxide (NO) to heme in the β1 subunit soluble guanylyl cyclase (sGC) activates both heterodimeric α1β1 and α2β1 isoforms enzyme, leading increased production cGMP from GTP. In cultured human mast cells, exogenous NO is able inhibit cell degranulation via NO-cGMP signaling. However, under inflammatory oxidative or nitrosative stress, sGC becomes insensitive NO. occurrence cells healthy inflamed tissues vivo expression α1 subunits during inflammation remain largely...
By the formation of cyclic guanosine 3′,5′-monophosphate (cGMP), nitric oxide (NO)-sensitive enzyme-soluble guanylate cyclase (sGC) plays a receptor role for NO within NO-cGMP signaling cascade, which is involved in vasodilatation and neurotransmission. The hypothesis that molecules modulate cells dentin-pulp complex was investigated rat molars by histochemical, immunohistochemical, immuno-ultrastructural, organ bath techniques. synthase (NOS) I-III, sGC α 2 -subunit/β 1 -subunit, cGMP were...
Abstract Odontoblasts and pulp stroma cells are embedded within supramolecular networks of extracellular matrix (ECM). Fibrillin microfibrils associated proteins crucial constituents these networks, serving as contextual scaffolds to regulate tissue development homeostasis by providing both structural mechanical properties sequestering growth factors the TGF-β superfamily. EMILIN-1, -2, -3 microfibril-associated glycoproteins known modulate cell behaviour, factor activity, ECM assembly. So...
The nitric oxide (NO)–sensitive soluble guanylyl cyclase (sGC) is a heterodimeric enzyme with an α and β subunit. NO binds to heme of the 1 -subunit sGC, activates in reduced iron state vascular smooth muscle cells (VSMCs), generates cGMP-inducing vasodilatation suppression VSMC proliferation. In complex tumor milieu higher levels reactive oxygen species (ROS), sGC may become oxidized insensitive NO. To change from NO-insensitive NO-sensitive or NO-independent manner, protein expression...
Various local and systemic factors compromise oral wound healing may lead to dehiscence, inflammation, or ulcers. Currently, there is a lack of topical therapeutical options. Thus, this study aimed investigate the effect Aloe vera (AV) Rheum palmatum root (RPR) on capacity in vitro. The AV RPR human primary fibroblast viability migration was studied by measuring metabolic activity gap closure scratch assay. Furthermore, cell cycle distribution cytoskeletal features were analyzed....
While the activation of eNOS by Akt/PKB-dependent phosphorylation, leading to NO release, and inhibition enzyme activity bradykinin (BK)-mediated phosphorylation in endothelial cells are established, odontoblasts is unknown. To clarify regulation BK, we examined eNOS, Akt/PKB, ERK1/2 rat molars. BK (10 −7 M) transiently induced at Ser 1177 , Akt/PKB odontoblasts, while it Thr 495 throughout entire period treatment. receptor 2 antagonist HOE 140 −6 significantly reduced signal intensities...
Knowledge of the histochemical substrates cellular and neurovascular connections in gingiva is essential order to understand initial mechanisms inflammation periodontium. Since localization neuroendocrine peptide galanin still unclear, we used immunohistochemical, situ hybridization immunoblot techniques assess rat molars. Galanin‐immunoreactive nerve fibers were located around blood vessels lamina propria, beneath epithelium, epithelial–proprial junction basal layer epithelium. Galanin was...
Background: Nitric oxide (NO) mediates different cellular functions by activating soluble guanylate cyclase (sGC) that converts guanosine‐5'‐ triphosphate (GTP) to cyclic guanosine‐3', 5'‐monophosphate (cGMP). Membrane‐bound GCs produce cGMP in response natriuretic peptides osteoblasts, but neither the NO‐target enzyme sGC, nor phosphorylation sites of NOS III, their regulation extracellular signalregulated kinases 1 and 2 (ERK1/2) Akt/protein kinase B (Akt/PKB) osteoclasts have been...
VEGF signaling regulated by the vascular endothelial growth factor receptor 2 (VEGFR2) plays a decisive role in tumor angiogenesis, initiation and progression several tumors including HNSCC. However, impact of HPV-status on expression VEGFR2 OPSCC has not yet been investigated, although HPV oncoproteins E6 E7 induce VEGF-expression. In series 56 with known HPV-status, patterns were analyzed both blood vessels from tumor-free tumor-containing regions within cells immunohistochemistry using...
The epithelial rests of Malassez (ERM) are derived from the disintegrating root sheath Hertwig that guides formation during tooth development. Low concentrations nitric oxide (NO) produced by NO-synthase I (NOS I) and NOS III activate intracellular soluble guanylate cyclase (sGC) to produce cyclic guanosine 3':5'-monophosphate (cGMP), which triggers rapid cellular responses such as cell proliferation, differentiation, apoptosis under physiological conditions. presence I-III, sGC (alpha2-...
Orthodontic tooth movement to therapeutically align malpositioned teeth is supposed impact blood flow in the surrounding tissues. Here, we evaluated actual vascularization tension area of periodontal ligament during experimental rats (N = 8) with magnetic resonance imaging (MRI). We inserted an elastic band between left upper first and second rat molar; right side was not treated served as control. After four days movement, recorded T1-weighted morphologic dynamic-contrast-enhanced MRI...
Havacılık alanında İnsansız Hava Araçlarına (İHA) yönelik talep ve çalışmalar tüm dünyada ülkemizde gün geçtikçe artmaktadır.İHA'lar, kumanda ya da uydu sistemleri aracılığıyla kontrol edilen, dikey olarak kalkış iniş yapabilen, uzun süreli uçuşları gerçekleştirebilen araçlardır.İHA'ların kullanım alanları arasında gözetleme, takip, nesne tanıma uzaktan müdahale sayılabilir.Günümüzde, çok rotorlu insansız hava araçları konusunda yapılan daha önem kazanmıştır.Dikey kalkış-iniş yapabilmesi...
Background: The extracellular signal‐regulated kinases 1 and 2 (ERK1/2) have been implicated in the inflammation‐dependent sensitization of nociceptors. Because periodontal ligament (PDL) contains numerous nociceptors mechanoceptors, phosphorylation ERK1/2 was investigated nerve fibers PDL to elucidate role constitutive local activation peripheral sensitization. Methods: Decalcified free‐floating sections rat molars with were incubated using total (t)‐ERK1/2 phosphorylated (p)‐ERK1/2...
The activity of endothelial nitric oxide synthase (eNOS) in cells increased with the phosphorylation enzyme at Ser1177 and decreased Thr495. regulation sites eNOS Thr495 blood vessels healthy inflamed human dental pulp is unknown. To investigate this, carious third molars were immersion-fixed decalcified. localization eNOS, Ser1177, was examined consecutive cryo-sections using quantitative immunohistochemical methods. We found that staining intensity vessels, whereas weak strongly vessels....