A5070154744- Breast Cancer Treatment Studies
- Cancer Immunotherapy and Biomarkers
- AI in cancer detection
- Breast Lesions and Carcinomas
- Bone Tumor Diagnosis and Treatments
- Cancer Genomics and Diagnostics
- Sarcoma Diagnosis and Treatment
- Radiomics and Machine Learning in Medical Imaging
- Adipokines, Inflammation, and Metabolic Diseases
- Bladder and Urothelial Cancer Treatments
- Cancer Risks and Factors
- Immune cells in cancer
- Immune Cell Function and Interaction
- DNA Repair Mechanisms
- BRCA gene mutations in cancer
- PARP inhibition in cancer therapy
- Adipose Tissue and Metabolism
- Epigenetics and DNA Methylation
- Cancer Cells and Metastasis
- Global Cancer Incidence and Screening
- Gene expression and cancer classification
- Genetics and Neurodevelopmental Disorders
- Molecular Biology Techniques and Applications
- Economic and Financial Impacts of Cancer
- Exercise and Physiological Responses
University of North Carolina at Chapel Hill
2016-2025
UNC Lineberger Comprehensive Cancer Center
2013-2021
Public Health Department
2016-2018
Dana-Farber Cancer Institute
2017
North Carolina Central University
2015
National Institute of Environmental Health Sciences
2014
University of Wollongong
1995
Abstract Macrophages are sentinel immune cells that survey the tissue microenvironment, releasing cytokines in response to both exogenous insults and endogenous events such as tumorigenesis. mediate tumor surveillance therapy-induced regression; however, tumor-associated macrophages (TAM) their products may also promote progression. Whereas NF-κB is prominent macrophage-initiated inflammatory responses, little known about role of p53 macrophage responses environmental challenge, including...
African American breast cancer patients have lower frequency of hormone receptor-positive (HR+)/human epidermal growth factor receptor 2 (HER2)-negative disease and higher subtype-specific mortality. Racial differences in molecular subtype within clinically defined subgroups are not well understood.Using data biospecimens from the population-based Carolina Breast Cancer Study (CBCS) Phase 3 (2008-2013), we classified 980 invasive cancers using RNA expression-based PAM50 recurrence (ROR)...
Abstract Background: OncotypeDx is a prognostic and predictive genomic assay used in early-stage hormone receptor–positive, HER2− (HR+/HER2−) breast cancer. It to inform adjuvant chemotherapy decisions, but not all eligible women receive testing. We aimed assess variation testing by demographics geography, determine whether was associated with chemotherapy. Methods: For 1,615 the Carolina Breast Cancer Study HR+/HER2−, Stage I–II tumors, we estimated prevalence differences (PD) 95%...
Classification of breast cancer into intrinsic subtypes has clinical and epidemiologic importance. To examine accuracy IHC-based methods for identifying subtypes, a three-biomarker IHC panel was compared with the record RNA-based (PAM50) subtypes.Automated scoring estrogen receptor (ER), progesterone (PR), HER2 performed on IHC-stained tissue microarrays comprising 1,920 cases from African American Breast Cancer Epidemiology Risk (AMBER) consortium. Multiple cores (1-6/case) were collapsed...
We examined racial differences in the expression of eight genes and their associations with risk recurrence among 478 white 495 black women who participated Carolina Breast Cancer Study Phase 3. tumor samples were analyzed for PAM50 subtype previously found to be differentially expressed by race associated breast cancer survival: ACOX2, MUC1, FAM177A1, GSTT2, PSPH, PSPHL, SQLE, TYMS. The these according was assessed using linear regression each gene evaluated association Cox regression....
Using a murine model of basal-like breast cancer, we tested the hypothesis that chronic obesity, an established cancer risk and progression factor in women, induces mammary gland epigenetic reprogramming increases tumor growth. Moreover, assessed whether obesity-induced protumor effects are reversed by weight normalization. Ovariectomized female C57BL/6 mice were fed control diet or diet-induced obesity (DIO) regimen for 17 weeks, resulting normal obese phenotype, respectively. Mice on DIO...
Abstract Purpose: Improved risk stratification and predictive biomarkers of treatment response are needed for non–muscle-invasive bladder cancer (NMIBC). Here we assessed the clinical utility targeted RNA DNA molecular profiling in NMIBC. Experimental Design: Gene expression NMIBC samples was profiled by NanoString nCounter, an quantification platform, from two independent cohorts (n = 28, n 50); panel sequencing performed a subgroup 50). signatures were externally validated using datasets...
Epidemiological studies linking dietary fat intake and obesity to breast cancer risk have produced inconsistent results. This may be due the difficulty of dissociating from obesity, and/or lack defined periods exposure in these studies. The pubertal mammary gland is highly sensitive cancer-causing agents. We assessed how high diet (HFD) affects inflammation, proliferative, developmental events gland, since dysregulation can promote tumorigenesis. To test effect HFD initiated during puberty...
<title>Abstract</title> Background Immune expression profiling in colorectal lesions may provide insights into the origins of antitumor immunity and senescence. Optimal approaches for analyzing samples with lower quality RNA from molecularly diverse are lacking. Therefore, we developed a NanoString nCounter-based approach control (QC), normalization, differential (DE) analysis, optimized FFPE contexts high biologic heterogeneity. Methods The incorporates colon specific positive gene set (11...
Breast cancer subtype can be classified using standard clinical markers (estrogen receptor (ER), progesterone (PR) and human epidermal growth factor 2 (HER2)), supplemented with additional markers. However, automated biomarker scoring classification schemes have not been standardized. The aim of this study was to optimize tumor methods in order describe frequency the African American Cancer Epidemiology Risk (AMBER) consortium. Using immunohistochemistry (IHC), we quantified expression ER,...
// Yirong Zhu 1 , Mark D. Aupperlee 2 Yong Zhao Ying Siow Tan Erin L. Kirk 4 Xuezheng Sun Melissa A. Troester 4, 5, 6 Richard C. Schwartz 3 Sandra Z. Haslam Cell and Molecular Biology Program Breast Cancer the Environment Research Program, Michigan State University, East Lansing, MI, USA Department of Physiology Microbiology Genetics Epidemiology, University North Carolina at Chapel Hill, NC, 5 Lineberger Comprehensive Center, Pathology Laboratory Medicine, Correspondence to: Haslam, email:...
Abstract Purpose: Chordoma is a rare bone tumor with high recurrence rate and limited treatment options. The aim of this study was to identify molecular subtypes chordoma that may improve clinical management. Experimental Design: We conducted RNA sequencing in 48 tumors from patients Chinese skull-base identified two major subtypes. then replicated the classification using NanoString panel North America. Results: Tumors one subtype were more likely have somatic mutations reduced expression...
Immunotherapy is a rapidly evolving treatment option in breast cancer; However, the cancer immune microenvironment understudied Black and younger (<50 years) patients.
Female breast cancer demonstrates bimodal age frequency distribution patterns at diagnosis, interpretable as two main etiologic subtypes or groupings of tumors with shared risk factors. While RNA-based methods including PAM50 have identified well-established clinical subtypes, diagnosis a proxy for subtype are not established molecular and genomic tumor classifications.We evaluated smoothed distributions Carolina Breast Cancer Study cases within immunohistochemistry-based expression...
Black women have higher hormone receptor positive (HR+) breast cancer mortality than White women. Early recurrence rates differ by race, but little is known about genomic predictors of early among HR+
Increased animal fat consumption is associated with increased premenopausal breast cancer risk in normal weight, but not overweight, women. This agrees our previous findings obesity-resistant BALB/c mice, which exposure to a high saturated diet (HFD) from peripuberty through adulthood promoted mammary tumorigenesis. Epidemiologic and studies support the importance of puberty as life stage when environmental exposures affect adult risk. In this study, we identified effects peripubertal HFD...
Abstract Background: Aberrant expression of DNA repair pathways such as homologous recombination (HR) can lead to imbalance, genomic instability, and altered chemotherapy response. imbalance may predict prognosis, but variation in diverse cohorts breast cancer patients is understudied. Methods: To identify RNA-based patterns expression, we performed unsupervised clustering on 51 repair-related genes the Cancer Genome Atlas Breast [TCGA BRCA (n = 1,094)] Carolina Study [CBCS 1,461)]. Using...
The tumor microenvironment is an important determinant of breast cancer progression, but standard methods for describing the are lacking. Measures composition such as stromal area and immune infiltrate labor-intensive few large studies have systematically collected this data. However, digital histologic approaches becoming more widely available, allowing high-throughput, quantitative estimation. We applied to tissue microarrays tumors from 1687 women (mean 4 cores per case) in Carolina...