Disturbances in the oral microbiome are associated with periodontal disease initiation and progression diabetes mellitus (DM), but how this contributes to cause-and-effect relationship between DM is poorly understood. We examined bacterial composition plaque samples from 128 South Africans across glycemic statuses using 16S rDNA sequencing of regions 2, 3, 4, 6-7, 8, 9. Of 9 phyla identified, Firmicutes, Proteobacteria, Bacteroidetes, Fusobacteria, Actinobacteria made up >98%....

microRNAs (miRNAs) have been touted as potential diagnostic and prognostic biomarkers for various diseases. The aim of the present study was to evaluate value miR-30a-5p miR-182-5p prediabetes screen-detected type 2 diabetes mellitus (T2DM).The included 1270 participants (207 prediabetes, 94 969 normotolerant) from Vascular Metabolic Health (VMH) study. Whole blood levels were quantitated by RT-qPCR. Multivariable logistic regressions used relate miRNAs with or T2DM receiver operating...

Glycated haemoglobin (HbA1c) has shown disagreements with other glycaemic indices; termed the glycation gap. The gap can be influenced by non-glycaemic factors like protein deglycation, through fructosamine 3 kinase (FN3K) enzyme. This cross-sectional study aimed to examine whether single nucleotide polymorphisms (SNPs) in FN3K gene explain Amongst 826 participants, 79.8% were female, 22.3% presented DM and median age was 53 years. results suggest that genetic may influence individuals...

<p dir="ltr">Glycated haemoglobin (HbA1c) has shown disagreements with other glycaemic indices; termed the glycation gap. The gap can be influenced by non-glycaemic factors like protein deglycation, through fructosamine 3 kinase (FN3K) enzyme. This cross-sectional study aimed to examine whether single nucleotide polymorphisms (SNPs) in FN3K gene explain Amongst 826 participants, 79.8% were female, 22.3% presented DM and median age was 53 years. results suggest that genetic may...

<p dir="ltr">Glycated haemoglobin (HbA1c) has shown disagreements with other glycaemic indices; termed the glycation gap. The gap can be influenced by non-glycaemic factors like protein deglycation, through fructosamine 3 kinase (FN3K) enzyme. This cross-sectional study aimed to examine whether single nucleotide polymorphisms (SNPs) in FN3K gene explain Amongst 826 participants, 79.8% were female, 22.3% presented DM and median age was 53 years. results suggest that genetic may...

Immune reconstitution following transplantation in individuals who had received T-cell-depleted marrow from HLA identical siblings was serially documented and correlated with the clinical recovery.Patients were preconditioned radiation containing programs. GvHD prophylaxis by T-cell depletion CAMPATH 1G (ex vivo; median dose 20 mg). After lymphoid development studied flow cytometry serum Ig concentrations determined. Charts reviewed to determine effects of immune on performance.The mean...

Background. Compared with allogeneic bone marrow, in cytokine mobilized blood stem cell grafts, the number of hematopoietic progenitors and lymphoid cells is higher. Consequently, we compared immune reconstitution following these two transplant modalities patients receiving T-cell depleted grafts for hematological malignancies studied impact lymphocyte subset recovery on clinical outcome. Methods. Conditioning transplantation was radiation-based, while graft versus host disease prophylaxis...

AIMS: To determine the prevalence of Pseudomonas fluorescens on arms blood donors, and to elucidate one possible cause for its predominance (60% cases during 1980-89) in exogenous post transfusion septicaemia (PTS). METHODS: Skin swabs were taken from 782 donors cultured heated agar. After incubation, Oxidase reagent Gram stain used select non-fermentative negative rods, which then subcultured identified using Analytical Profile System (API) 20 NE system. RESULTS: Non-fermentative rods found...

This cross-sectional study investigated the association of miR-1299, -126-3p and -30e-3p with their diagnostic capability for dysglycaemia in 1273 (men, n = 345) South Africans, aged >20 years. Glycaemic status was assessed by oral glucose tolerance test (OGTT). Whole blood microRNA (miRNA) expressions were using TaqMan-based reverse transcription quantitative-PCR (RT-qPCR). Receiver operating characteristic (ROC) curves ability each miRNA to discriminate dysglycaemia, while multivariable...

Allogeneic stem cell transplantation (SCT) is a critical therapy for haematological malignancy but may lead to acute and chronic graft versus host disease (GvHD). T-cell depletion with alemtuzumab, either in vivo or ex vivo, reduces the incidence of GvHD risk factor relapse poor immune reconstitution. Natural killer (NK) cells are first lymphocytes recover. Classical NK make up >90% normal circulating population can directly kill neoplastic virally infected while regulatory subset makes...

Aim: The influence of disease duration and anti-diabetic treatment on epigenetic processes has been described, with limited focus interactions microRNAs (miRNAs). miRNAs have found to play key roles in the regulation pathways associated type 2 diabetes mellitus (T2DM), expression patterns response may further promote their use as therapeutic targets T2DM its complications. We therefore aimed investigate expressions circulating (miR-30a-5p, miR-1299, miR-182-5p, miR-30e-3p miR-126-3p) newly...

Summary Introduction Cardiovascular disease and thrombotic events have emerged as major causes of mortality in people living with HIV . Activated platelets play a key role both inflammation thrombosis. Haematology analysers measure variety platelet indices, which could be surrogate markers activation. Flow cytometry offers the discrimination subpopulations evaluation activation status platelets. This study aimed to indices untreated infection evaluate their relationship immune progression....

MicroRNAs are non-coding, post-transcriptional regulators of gene expression and their dysregulation has been associated with development various diseases, including hypertension. Consequently, understanding role in the pathogenesis progression disease is essential. Prior research focusing on microRNAs provided a basis for prognosis offered possible channels therapeutic interventions. Herein, we aimed to investigate differences profiles five blood participants grouped hypertension status....

Abstract Background Research has suggested that individuals of African descent have lower white cell and neutrophil counts than Caucasians. These differences could lead to incorrect clinical decisions, therefore, ethnic‐specific reference ranges are required. The Western Cape region South Africa is uniquely diverse, comprising Caucasian, Mixed Ancestry those descent. aim this study was compare the full blood count differential across three major ethnic groups residing in area propose...

The oral microbiota plays a crucial role in both systemic inflammation and metabolic syndrome (MetS), which is characterised by low-grade inflammation. Studies have analysed the gut using stool specimens from subjects with MetS; however, etiological of development MetS still uncertain. We investigated 128 subgingival plaque samples South African cohort without MetS. After comprehensive analysis microbiota, we observed significant increase Gram-positive aerobic anaerobic those an abundance...

Abstract Platelet activation has been described in patients with chronic inflammation, however type 2 diabetes mellitus it remains controversial. We compared levels of platelet leucocyte aggregates, monocyte and granulocyte across glucose tolerance statuses mixed ancestry South Africans. Individuals (206) were recruited from Bellville-South, Cape Town, included 66% normal tolerance, 18.7% pre-diabetes, 8.7% screen-detected 6.3% known diabetes. Monocyte neutrophil measured by calculating the...

Human immunodeficiency deficiency virus (HIV) infection is associated with chronic inflammation and an increased risk of thrombotic events. Activated platelets (PLTs) play important role in both thrombosis inflammation, HIV has been shown to induce PLT activation by direct indirect mechanisms. P-selectin (CD62P) a well-described marker activation, glycoprotein (GP) IV (CD36) identified as aggregation. Data on function the context remain inconclusive. Laboratory techniques, such flow...