A5006839622- Animal Genetics and Reproduction
- CRISPR and Genetic Engineering
- RNA Research and Splicing
- RNA modifications and cancer
- Aquaculture disease management and microbiota
- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- Reproductive biology and impacts on aquatic species
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Microtubule and mitosis dynamics
- Circular RNAs in diseases
- Developmental Biology and Gene Regulation
- Protein Hydrolysis and Bioactive Peptides
- RNA and protein synthesis mechanisms
- Fungal and yeast genetics research
- Extracellular vesicles in disease
- Autophagy in Disease and Therapy
- TGF-β signaling in diseases
- Plant Genetic and Mutation Studies
- Aquaculture Nutrition and Growth
- DNA Repair Mechanisms
- GABA and Rice Research
- Cancer-related gene regulation
- Meat and Animal Product Quality
- Ginseng Biological Effects and Applications
Changchun University of Chinese Medicine
2016-2023
Central China Normal University
2023
University of Edinburgh
2019-2022
Roslin Institute
2019-2022
Jiangsu University
2022
Affiliated Hospital of Jiangsu University
2022
Tongde Hospital of Zhejiang Province
2017-2022
Inner Mongolia Agricultural University
2022
University of Stirling
2018-2020
Harbin Medical University
2020
Genetic resistance to infectious pancreatic necrosis virus (IPNV) in Atlantic salmon is a rare example of trait where single locus (QTL) explains almost all the genetic variation. marker tests based on this QTL chromosome 26 have been widely applied selective breeding markedly reduce incidence disease. In current study, whole genome sequencing and functional annotation approaches were characterise genes variants region. This was complemented by an analysis differential expression between fry...
The application of genome engineering techniques to understand the mechanisms that regulate germ cell development opens promising new avenues develop methods control sexual maturation and mitigate associated detrimental effects in fish. In this study, functional role piwil2 primordial cells (PGCs) was investigated Nile tilapia using CRISPR/Cas9 resultant genotypes were further explored. is a gonad-specific maternally deposited gene eggs which known play repression transposon elements...
The regulation and timing of spindle pole body (SPB) duplication maturation in fission yeast was examined by transmission electron microscopy. When cells are arrested at G1 nitrogen starvation, the SPB is unduplicated. On release from G1, SPBs were duplicated after 1-2 h. In S hydroxyurea, but not mature. arrest/release experiments with cdc2.33 restrictive temperature, remained single, whereas permissive duplicated. cdc10 mutant cells, seem only to be also undergo partial maturation,...
Abstract Infectious and parasitic diseases have major negative economic animal welfare impacts on aquaculture of salmonid species. Improved knowledge the functional basis host response genetic resistance to these is key developing preventative treatment options. Cell lines provide valuable models study infectious in salmonids, genome editing using CRISPR/Cas systems provides an exciting avenue evaluate function specific genes those systems. While has been successfully performed a Chinook...
As a common adverse environmental factor, heat stress (HS) not only drastically changes the plant transcriptome at transcription level but also increases alternative splicing (AS), especially intron retention (IR) events. However, exact mechanisms are yet well understood. Here, we reported that NTC-related protein 1 (NTR1), which acts as an accessory component for spliceosome disassembly, is necessary this process. The mutants of NTR1, both T-DNA insertion and point mutation identified...
Abstract In meiotic prophase of many eukaryotic organisms, telomeres attach to the nuclear envelope and form a polarized configuration called bouquet. Bouquet formation is hypothesized facilitate homologous chromosome pairing. fission yeast, bouquet telomere clustering occurs in karyogamy persists throughout horsetail stage. Here we report isolation characterization six mutants from our screen for mutants. These show defective as demonstrated by mislocalization Swi6::GFP,...
The telomere bouquet, i.e., clustering on the nuclear envelope (NE) during meiotic prophase, is thought to promote homologous chromosome pairing. Using a visual screen, we identified bqt2/im295, mutant that disrupts in fission yeast. Bqt2p required for linking telomeres spindle pole body (SPB) but not attachment of or SPB NE. expressed upon pheromone sensing and colocalizes thereafter Sad1p, an protein. This localization only depends Bqt1p, other proteins clustering. Upon sensing, generation...
The role of genetic abnormality δ-sarcoglycan (δ-SG) gene in dilated (DCM) and hypertrophied (HCM) cardiomyopathy patients is still unfolding. In this study we first defined the promoter region then searched for polymorphisms/mutations among promoter, 5'-untranslated region, encoding exons δ-SG 104 Chinese with DCM, 145 HCM, 790 normal controls. Two novel polymorphisms were found, an 11 base-pair (bp) deletion (c.-100~-110; -) a missense polymorphism A848G resulting p.Q283R highly conserved...
Background The survival rate of septic patients mainly depends on a rapid and reliable diagnosis. A rapid, broad range, specific sensitive quantitative diagnostic test is the urgent need. Thus, we developed TaqMan‐Based Multiplex real‐time PCR assays to identify bloodstream pathogens within few hours. Methods Primers TaqMan probes were designed be complementary conserved regions in 16S rDNA gene different kinds bacteria. To evaluate accurately, sensitively, specifically, known bacteria...
The action of melatonin within the body animals is known to be mediated by receptors. Three different types receptors have been identified so far in fish. However, which these are specifically involved puberty onset not We cloned and analyzed sequence receptor 1a (mel 1a) gene Nile tilapia Oreochromis niloticus. In addition, we examined tissue distribution expressions for three receptors, mel 1a, 1b lc investigated them comparing their expression with that gonadotropin-releasing hormone I...