A5059429984- Angiogenesis and VEGF in Cancer
- Cell Adhesion Molecules Research
- Retinal Diseases and Treatments
- Retinal Development and Disorders
- Cerebrovascular and genetic disorders
- Advanced optical system design
- Peptidase Inhibition and Analysis
- Glycosylation and Glycoproteins Research
- Advanced Glycation End Products research
- Coronary Interventions and Diagnostics
- Nitric Oxide and Endothelin Effects
- Barrier Structure and Function Studies
- Proteoglycans and glycosaminoglycans research
- Image and Object Detection Techniques
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Biochemical and Molecular Research
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Ocular and Laser Science Research
- Advanced Optical Sensing Technologies
- Image Processing and 3D Reconstruction
- Adaptive optics and wavefront sensing
- Signaling Pathways in Disease
- Melanoma and MAPK Pathways
- Immune Response and Inflammation
- RNA modifications and cancer
Boston University
2019-2024
Harvard University
2013-2023
Smith-Kettlewell Eye Research Institute
2013-2023
Massachusetts Eye and Ear Infirmary
2013-2023
Chinese Academy of Medical Sciences & Peking Union Medical College
2020
Wuhan University
2005-2019
The University of Texas MD Anderson Cancer Center
2009-2013
Augusta University
2006-2013
Discovery Institute
2013
Augusta University Health
2011-2012
Abstract Endomucin is a membrane-bound glycoprotein expressed luminally by endothelial cells that line postcapillary venules, primary site of leukocyte recruitment during inflammation. Here we show endomucin abrogation on quiescent enables neutrophils to adhere firmly, via LFA-1-mediated binding ICAM-1 constitutively cells. Moreover, TNF-α stimulation downregulates cell surface expression concurrent with increased adhesion molecules. Adenovirus-mediated under inflammatory conditions prevents...
Angiogenesis is central to both normal and pathologic processes. Endothelial cells (ECs) express O-glycoproteins that are believed play important roles in vascular development stability. Endomucin-1 (EMCN) a type I O-glycosylated, sialic-rich glycoprotein, specifically expressed by venous capillary endothelium. Evidence has pointed potential role for EMCN angiogenesis but it had not been directly investigated. In this study, we examined the of modulating levels vivo vitro. Reduction led...
Pigment epithelium-derived factor (PEDF) is a potent inhibitor of vascular endothelial growth (VEGF)-induced permeability. The goal this study was to understand the mechanism by which PEDF blocks VEGF-induced increases in permeability.The paracellular permeability bovine retinal (BRE) cells measured assaying transendothelial cell electrical resistance and tracer flux. Western blot analysis used show phosphorylation VEGFR2, MAP kinases, glycogen synthase kinase 3 (GSK3)-beta. Confocal imaging...
Angiogenic sprouting is a critical process involved in vascular network formation within tissues. During sprouting, tip cells and ensuing stalk migrate collectively into the extracellular matrix while preserving cell–cell junctions, forming patent structures that support blood flow. Although several signaling pathways have been identified as controlling it remains unclear to what extent this mechanoregulated. To address question, we investigated role of cellular contractility sprout...
Diabetes-induced breakdown of the blood-retinal barrier (BRB) has been linked to hyperglycemia-induced expression vascular endothelial growth factor (VEGF) and is likely mediated by an increase in oxidative stress. We have shown that VEGF increases permeability retinal cells (REC) inducing urokinase plasminogen activator receptor (uPAR). The purpose this study was define role superoxide anion VEGF/uPAR BRB diabetes. Studies were performed streptozotocin diabetic rats mice high glucose (HG)...
Contact between inflammatory cells and endothelial (ECs) is a crucial step in vascular inflammation. Recently, we demonstrated that the cell-surface level of endomucin (EMCN), heavily O-glycosylated single-transmembrane sialomucin, interferes with interactions ECs. We have also shown that, response to an stimulus, EMCN cleared from cell surface by unknown mechanism. In this study, using adenovirus-mediated overexpression tagged human umbilical vein ECs, found treatment tumor necrosis factor...
In the nonhuman primate and human corpora lutea, gap junctions have been identified by means of electron microscopy. Gap are formed connexons, which consist a multigene family tissue-specific connexins. ovarian follicle, junction protein connexin-43 is present hormonally regulated. However, there little evidence indicating type connexin in corpus luteum. Therefore, aim this study was to demonstrate presence microscopy messenger ribonucleic acid (mRNA) for protein. Using immunocytochemical...
Abstract Background The extended DNA fiber preparation procedure is still imperfect in plants due to the existence of a hard cell wall; thus, high quality fibers for fluorescence situ hybridization (FISH) analysis often difficult be obtained rapidly and efficiently. In this study we have developed fast widely effective method prepare from various plant species are suitable FISH mapping. Methods Fresh young leaves were chopped with sharp sterile scalpel Petri dish that contained ice‐cold...
In urban roads, the safety service of vehicular ad hoc networks is particularly important because frequent traffic accidents. order to minimise redundancy and delay network ensure high reception rate vehicles in target area when spreading emergency message. this study, authors propose a reliable broadcast mechanism based on layout information road. Different from existing mechanisms, straight, proposed regards effective communication distance prediction rather than fix as one parameters...
In this paper, a kind of efficient solution to the trihedral corner reflector's (TCR's) radar cross section (RCS) is proposed. Based on combination geometrical optics (GO) and Gordan surface integral method, TCR's RCS was computed compared with results from computatio nal electromagnetics software FEKO. Good agreement significant speedup achieved show that method mentioned in paper capable practical rapid simulation design, especially suitable for electrically large plate structure.
A 2.1 km terrestrial free-space optical data link operating at 5 Gbps by using WDM and 1550 nm fiber communication techniques was established. Two 2.5 pattern generators are used to directly modulate 1530 DFB lasers respectively. The output of each modulated signal combined a CWDM, then amplified 20dBm EDFA. is applied specially designed transmitting telescope. received focused onto the core 62.5μm multi-mode coupling optics within receive coupled interfaced de-multiplexer separate two...
Angiogenesis is central to both normal and pathologic processes. Endothelial cells (ECs) express high levels of O‐glycoproteins that are believed play important roles in vascular development stability. Endomucin‐1 (EMCN) a type I O‐glycosylated, sialic‐rich glycoprotein, specifically expressed by venous capillary endothelium. Although ECs EMCN, its possible role has not been examined. A for EMCN angiogenesis was studied modulating gene expression vitro vivo. Postnatal day (P) four C57BL/6...
Cube-corner retroreflector (CCR) has been widely used in the satellite laser ranging (SLR) systems as a cooperative target based on its direct reflecting. It can increase energy of pulse which is reflected back from satellite, augment distance between and observing station, improve accuracy. In recent years, CCR become one essential loads satellites for improving orbit's diffracts redistributes pulse, far-field diffraction impacts performance SLR greatly. this paper method detecting...