A5053384619- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- Circular RNAs in diseases
- Extracellular vesicles in disease
- RNA modifications and cancer
- Ferroptosis and cancer prognosis
- Cancer Immunotherapy and Biomarkers
- Immune cells in cancer
- Gut microbiota and health
- Inflammatory Biomarkers in Disease Prognosis
- RNA Research and Splicing
- Monoclonal and Polyclonal Antibodies Research
- Kruppel-like factors research
- RNA Interference and Gene Delivery
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Sepsis Diagnosis and Treatment
- Cancer-related gene regulation
- Galectins and Cancer Biology
- Hepatitis Viruses Studies and Epidemiology
- Mesenchymal stem cell research
- Peptidase Inhibition and Analysis
- Cancer, Hypoxia, and Metabolism
- Neuropeptides and Animal Physiology
- Epigenetics and DNA Methylation
- Cancer-related Molecular Pathways
Tongji University
2017-2025
Tongji Hospital
2017-2025
The First People’s Hospital of Lianyungang
2022-2025
Bengbu Medical College
2023-2025
Xian Yang Central Hospital
2022-2025
Xuzhou Medical College
2023-2025
The First Affiliated Hospital, Sun Yat-sen University
2025
Zhongshan Hospital
2025
Fudan University
2025
Sun Yat-sen University
2025
Abstract Background Colorectal cancer (CRC) is the leading cause of cancer-related death worldwide. Exosome shave emerged as crucial regulators intercellular communication and that abundant Circular RNAs (circRNAs) are enriched within exosomes. CircRNAs novel members noncoding regulating proliferation progression. However, function regulatory mechanism cancer-derived exosomal circRNAs in CRC remains unclear. Methods cells-derived exosomes were characterized using transmission electron...
Abstract Background Long non-coding RNA (lncRNA) HOXA transcript at the distal tip (HOTTIP), has been demonstrated to be a vital biomarker when evaluating prognosis of multiple cancers. Nevertheless, potential function HOTTIP in ovarian cancer (OC), prevalent among women worldwide, remains elusive. Hence, current study aimed elucidate functional relevance development OC. Methods Positive expression PD-L1 and IL-6 was determined using immunohistochemical staining collected OC normal tissues....
Abstract Background Colorectal cancer (CRC) remains one of the leading causes cancer-related death. The current study aimed to elucidate mechanism by which exosomes carrying KRAS mutant contribute neutrophil recruitment as well formation extracellular trap (NET) in CRC. Methods APC-WT and APC-KRAS G12D mouse models were initially developed. Peripheral blood, spleen, bone marrow (BM) mesenteric lymph nodes (mLN) isolated detect content. Then, mice injected with from mice. ratio neutrophils,...
Exosomes play important roles in proliferation and microenvironment modulation of many types cancers, including colorectal cancer (CRC).However, the inhibitory effect CRC cells-derived exosomes angiogenesis has not been fully discussed.In this study, microRNA-183-5p (miR-183-5p) abundant secreted from cells were investigated.Initially, microarray analysis was employed to determine differentially expressed miRNAs.Exosomes isolated co-cultured with HMEC-1 explore role angiogenesis.Further,...
To explore the effect of insulin-like growth factor 2 mRNA-binding protein (IGF2BP2) on colorectal cancer (CRC) by recognizing m6A modification YAP mRNA thus activating ErbB2 expression. High expressions IGF2BP2, YAP, and promoted proliferation, migration invasion CRC cells reduced their apoptosis. IGF2BP2 recognized translation mRNA. regulated expression promoting TEAD4 enrichment in promoter region. Therefore, to enhance cells, repress cell apoptosis, promote solid tumor formation nude...
Mesenchymal stem cells (MSCs) have shown great potential in the treatment of several inflammatory diseases due to their immunomodulatory ability, which is mediated by exosomes secreted MSCs (MSC-Exs). The incidence bowel disease (IBD) increasing globally, but there currently no long-term effective treatment. As an emerging therapy, MSC-Exs proven be alleviating IBD experimentally, and specific mechanism continues explored. gut microbiota plays important role occurrence development IBD, can...
Long noncoding RNAs (lncRNAs) have been reported to be involved in the occurrence and tumorigenesis of numerous malignant cancers. Microarray expression profiles were used screen colorectal cancer (CRC)-related differentially expressed genes lncRNAs, which revealed that insulin receptor substrate 1 (IRS1) lncRNA plasmacytoma variant translocation (PVT1) highly CRC. This study aimed investigate regulatory role PVT1 Subcellular localization detected by fluorescence situ hybridization...
Accumulating evidence indicates that INHBA (Inhibin β-A, a member of the TGF-β superfamily) functions as an oncogene in cancer progression. However, little is known to how regulates progression and aggressiveness breast (BC). This study explored function underlying mechanism epithelial–mesenchymal transition (EMT) BC cells. expression cell lines was measured using RT-qPCR Western blot. The would-healing transwell migration assays were used investigate effect overexpression or silencing on...
Recently, antibody-based therapeutic agents are becoming most leading biologics for treating many diseases, especially cancer. However, large-scale application of antibody drugs is still hampered by high cost and complex technical process. Endogenous expression proteins or antibodies can be achieved applying in vitro transcription (IVT) technique to produce mRNA then deliver into body, which supplies opportunity avoid disadvantages production as well clinical applications. Here, we designed...
Abstract This study aimed at elucidating the molecular mechanism of miR‐381‐3p in cervical cancer progression, which may provide a novel therapeutic target for patients with cancer. The expression was confirmed by quantitative reverse transcription polymerase chain reaction. Microarray analysis conducted to screen out differentially expressed genes, and gene microRNA (miRNA) predicted on TargetScan. Dual‐luciferase reporter assay then verified targeting relationship between FGF7 . protein...
Objective: The study aims to explore the relationship between expressions of HER2 and JAK/STAT3-SOCS3 signaling pathway clinicopathological features prognosis ovarian cancer (OC).Methods: A total 136 OC patients were collected. Immunohistochemistry was applied measure STAT3, p-STAT3, SOCS3, p-HER2 in tumor tissues adjacent normal tissues. Quantitative real-time polymerase chain reaction (qRT-PCR) used detect mRNA HER2, SOCS3 STAT3 western blotting for protein p-HER2, p-STAT3 Flow cytometry...
The involvement of cellular immunity in the development hepatitis E virus (HEV) infection is rare. We aimed to study roles viral load and Th cell responses acute (AHE) HEV-related liver failure (HEV-ALF).We evaluated Th1/Th2 cytokine levels 34 patients with HEV infection, including 17 each AHE or HEV-ALF. Seventeen healthy controls (HCs) were also included who negative for anti-HEV IgM IgG.There was no significant difference RNA HEV-ALF groups (both P > 0.05). lymphocyte (CD3+, CD4+)...
Long noncoding RNAs (lncRNAs), >200 nucleotides in length, are newly discovered non-coding RNA molecules that rarely translated into proteins [3].Recently, many lncRNAs shown to regulate the progression of important tumors, including ability proliferation, apoptosis, metastasis, senescence, metabolism and drug-resistance [4].To date, although a number have been functionally www.aging-us.com
Objectives: This study investigated plasma exosomal miRNA-139-3p as a blood-based biomarker for the early diagnosis and metastasis monitoring of colorectal cancer (CRC).Patients Methods: Exosome-rich fractions were isolated from 80 CRC patients, 23 controls using kit method.We then used real-time polymerase chain reaction (RT-qPCR) to detect miR-139-3p levels in all subjects evaluate expression predictive value CRC.We also collected clinicopathological data explore correlations between...
Purpose. Bladder cancer is one of the most common urological malignancies worldwide, and approximately 90% bladder cases are histologically typed as urothelial carcinoma (BLCA). Exosomes 30 to 200 nm extracellular vesicles that transport microRNAs, long noncoding RNAs (lncRNAs), mRNAs, circular RNAs, proteins across tissues through circulation. Urinary exosomes may contain genetic information from tumor cells. Herein, we explored clinical significance urinary exosomal lncRNA telomerase RNA...
The present study was aimed at defining the roles of intracellular triglyceride pools in apolipoprotein B secretion from HepG2 cells. Oleic acid (0.2 mmol/L) medium stimulated both synthesis and secretion. Stimulation lost about 30-40 min after oleic removed medium, despite finding that most newly synthesized still This suggested only a small fraction transferred to pool available for assembly nascent apoB into lipoproteins. Using cell fractionation, we analyzed two cells: microsomal...
Rationale: Antimicrobial peptides, such as cathelicidin LL-37/hCAP-18, are important effectors of the innate immune system with direct antibacterial activity.In addition, LL-37 is involved in regulation tumor cell growth.However, molecular mechanisms underlying functions promoting lung cancer not fully understood.Methods: The expression tissues and sera patients non-small was determined through immunohistological, immunofluorescence analysis, enzyme-linked immunosorbent assay.The animal...
Objective The purpose of this study is to determine the diagnostic value and net clinical benefit interleukin-10 (IL-10), interleukin-17 (IL-17), procalcitonin (PCT), combination tests in patients with sepsis, which will serve as a standard for sepsis early detection. Patients methods An investigation 84 81 local inflammatory diseases admitted ICU Tongji University Hospital 2021. In addition comparing inter-group variability, indicators relevant diagnosis therapy were screened. Results LASSO...
Disulfidptosis, a newly discovered cell death mode distinct from other programmed in lung and kidney cancer cells, is defined as extensive disulfide bonds to actin cytoskeleton proteins, leading contraction cytoskeletal disruption death. New pattern discoveries often drive advances tumor research. Therefore, the present study attempted decipher manifestation importance of disulfidptosis pan-cancer. Combining Clinical specimen immunofluorescence staining, single-cell analyses, spatial...
Abstract Osteosarcoma (OS), the most common primary bone tumor in children and young adults, poses significant clinical challenges. In this study, we investigated role of exosomes—key mediators intercellular communication—in OS progression identified potential therapeutic targets. Functional assays cellular animal models demonstrated that OS‐derived exosomes markedly enhanced proliferation, migration, invasion. Data‐independent acquisition‐based mass spectrometry analysis revealed a notable...