We present a theoretical analysis and experimental demonstration of particle trapping manipulation around optothermally generated bubbles. show that located within 500 μm surface bubble can be attracted towards by drag force resulting from convective flow. Once the comes in contact with bubble's surface, balance between tension forces pressure traps on allowing to move without detaching. The proposed mechanism is confirmed computational fluid dynamics simulations, calculations, experiments....

In this work, we develop an in situ method to grow highly controllable, sensitive, three-dimensional (3D) surface-enhanced Raman scattering (SERS) substrates via optothermal effect within microfluidic devices. Implementing approach, fabricate SERS composed of Ag@ZnO structures at prescribed locations inside channels, sites which current fabrication has been arduous. Conveniently, properties the 3D nanostructures such as length, packing density, and coverage can also be adjusted by tuning...

In this article, we demonstrated a handheld smartphone fluorescence microscope (HSFM) that integrates dual-functional polymer lenses with smartphone. The HSFM consists of smartphone, field-portable illumination source, and lens performs both optical imaging filtering. Therefore, compared the existing microscope, does not need any additional filters. Although has traditionally played an indispensable role in biomedical clinical applications due to its high specificity sensitivity for...

Gold nanoparticles (AuNPs) bound with biomolecules have emerged as suitable biosensors exploiting unique surface chemistries and optical properties. Many efforts focused on antibody bioconjugation to AuNPs resulting in a sensitive bioconjugate detect specific types of bacteria. Unfortunately, bacteria thrive under various harsh environments, an understanding stability is needed. Here, we show method for optimizing Listeria monocytogenes polyclonal antibodies mechanisms via covalent binding...

After half a billion years of evolution, arthropods have developed sophisticated compound eyes with extraordinary visual capabilities that inspired the development artificial eyes. However, limited 2D nature most traditional fabrication techniques makes it challenging to directly replicate these natural systems. Here, we present biomimetic apposition eye fabricated using microfluidic-assisted 3D-printing technique. Each microlens is connected bottom planar surface via intracorporal,...

A new strategy to achieve large-scale, three-dimensional (3D) micro- and nanostructured surface patterns through selective electrochemical growth on monolayer colloidal crystal (MCC) templates is reported. This method can effectively create large-area (>1 cm2), 3D with well-defined structures in a cost-effective time-saving manner (<30 min). variety of patterns, including semishells, Janus particles, microcups, mushroom-like clusters, generated. Most importantly, our be used prepare...

Non-small cell lung cancer (NSCLC) is the leading cause of cancer-related death worldwide. However, science has not yet been able to substantially improve prognosis patients. Accumulating evidence suggests that microRNAs (miRNAs) are key players in regulation tumor development and metastasis. Expression six miRNAs previously shown play roles (miR-146b-5p, miR-128b, miR-21, miR-221, miR-34a, Let-7a) other types was examined using real-time RT-PCR 78 specimens NSCLC. The results revealed...

An acoustically actuated, bubble-based technique is developed to investigate the deformability of cells suspended in microfluidic devices. A microsized bubble generated by an optothermal effect near targeted cells, which are a chamber. Subsequently, acoustic actuation employed create localized streaming. In turn, streaming flow results hydrodynamic forces that deform situ. The indicative their mechanical properties. method this study measures biomarkers from multiple single experiment, and...

The microRNA miR‐182, belonging to the miR‐183 family, is one of most frequently studied cancer‐related oncogenic miRNAs that dysregulated in various cancer tissues, and it plays a crucial role tumorigenesis tumor progression. Studies revealed miR‐182 might function as an or suppressor miRNA different tissues. However, development lung remains largely unknown. expression samples from 58 patients, normal tissue samples, cell lines were evaluated by qRT‐PCR. Survival curves analyzed using...

The Talbot effect of the surface plasmon polaritons (SPPs) using SPP launching gratings is studied experimentally. carpets are obtained and distance given when paraxial approximation not satisfied. Multi-layer multi-level-phase designed to enhance intensities amplitude-modulated revivals. Effective focusing SPPs with multiple focal spots a subwavelength full width at half maximum by three-layer four-level-phase grating.

We propose a refractive index sensor based on the interference of two surface-plasmon waves both surfaces gold film with two-slit structure. The phase was solved, and dispersion relation real part Au dielectric function considered. performed NaCl-H2O solutions different concentrations exhibited linear response high sensitivity 4547 nm/RIU (refractive unit) to change.

We present a programmable, biocompatible technique for dynamically concentrating and patterning particles cells in microfluidic device. Since our utilizes opto-thermally generated, acoustically activated, surface bubbles, we name it "optoacoustic tweezers". The optoacoustic tweezers are capable of particles/cells at any prescribed locations chamber without the use permanent structures, rendering particularly useful formation flexible, complex cell patterns. Additionally, this has...

Two-dimensional plasmonic demultiplexers for surface plasmon polaritons (SPPs), which consist of concentric grooves on a gold film, are proposed and experimentally demonstrated to realize light−SPP coupling, effective dispersion, multiple-channel SPP guiding. A resolution as high 10 nm is obtained. The leakage radiation microscopy imaging shows that the SPPs different wavelengths focused routed into strip waveguides. demultiplexer can thus serve wavelength division multiplexing element an...

Hydroxycancrinite and analcime were generated in the reaction process of K-feldspar various concentration solutions Na₂SiO₃.

A heterogeneous optical slot antenna significantly enhances the single-molecule excitation and emission rate, which enables detection in solutions at high concentrations, compared to a conventional zero-mode waveguide.

The ability of zero-mode waveguides (ZMWs) to guide light energy into subwavelength-diameter cylindrical nanoapertures has been exploited for single-molecule fluorescence studies biomolecules at micromolar concentrations, the typical dissociation constants biomolecular interactions. Although epi-fluorescence microscopy is now adopted ZMW-based imaging as an alternative commercialized ZMW platform, its suitability and performance awaits rigorous examination. Here, we present conical...

Single-molecule detection has become an indispensable technology in life science, and medical research. In order to get meaningful information on many biological processes, single-molecule analysis is required micro-molar concentrations. At such high concentrations, it very challenging isolate a single molecule with conventional diffraction-limited optics. Recently, optical aperture nanoantennas (OANs) have emerged as powerful tool enhance the under physiological environment. The OANs, which...

Guiding analytes to the sensing area is an indispensable step in a system. Most of systems apply passive method, which waits for diffuse towards sensor. However, methods limit detection picomolar range on micro/nanosensors practical time scale. Therefore, active need be used improve are forced concentrate sensors. In this article, we have demonstrated manufacturing nanogap-rich structures chemical sensing. Nanogap-rich manufactured from metallic nanoparticles through optothermally generated...

We demonstrate an opto-thermomechanical (OTM) nanoprinting method that allows us not only to additively print nanostructures with sub-100 nm accuracy but also correct printing errors for nanorepairing under ambient conditions. Different from other existing methods, this works when a nanoparticle on the surface of soft substrate is illuminated by continuous-wave (cw) laser beam in gaseous environment. The heats and induces rapid thermal expansion substrate. This can either release or transfer...

We propose a binary plasmonic method to design the structure (BPS), which is used launch surface plasmon polaritons (SPPs) desired pattern. The BPS composed of pixelated grooves. When plane wave illuminates perpendicularly, relative phases launched SPPs are determined by positions grooves, designed simulated annealing algorithm. Here, we obtain single, three, and five SPP focal spots along line parallel BPS, respectively, three spots, located at apexes triangle. experiment agrees well with...

Optical imaging with nanoscale resolution and a large field of view is highly desirable in many research areas. Unfortunately, it challenging to achieve these two features simultaneously while using conventional microscope. An objective lens low numerical aperture (NA) has but poor resolution. In contrast, high NA will have higher reduced view. an effort close the gap between trade-offs, we introduce acoustofluidic scanning nanoscope (AS-nanoscope) that can The AS-nanoscope relies on...