A5062357055- Microfluidic and Bio-sensing Technologies
- Microfluidic and Capillary Electrophoresis Applications
- Electrowetting and Microfluidic Technologies
- Acute Myocardial Infarction Research
- Nanopore and Nanochannel Transport Studies
- 3D Printing in Biomedical Research
- Nanofabrication and Lithography Techniques
- Sepsis Diagnosis and Treatment
- Antiplatelet Therapy and Cardiovascular Diseases
- Microbial Inactivation Methods
- Advanced Surface Polishing Techniques
- Extracellular vesicles in disease
- Atrial Fibrillation Management and Outcomes
- Injection Molding Process and Properties
- Heart Failure Treatment and Management
- Acoustic Wave Resonator Technologies
- Orbital Angular Momentum in Optics
- Advanced optical system design
- Blood Pressure and Hypertension Studies
- Inflammation biomarkers and pathways
- Gut microbiota and health
- Cardiovascular Health and Disease Prevention
- Electrohydrodynamics and Fluid Dynamics
- Supramolecular Self-Assembly in Materials
- Magnetic and Electromagnetic Effects
Massachusetts Institute of Technology
2021-2025
Taipei Veterans General Hospital
2005-2024
National Yang Ming Chiao Tung University
2005-2024
Taiwan Textile Research Institute
2024
National Chin-Yi University of Technology
2023
Duke University
2016-2022
National Taiwan University
2007-2021
Durham Technical Community College
2018-2021
National Chung Hsing University
2006-2021
National Chiayi University
2021
Significance We have developed a unique, integrated, on-chip technology that is capable of isolating exosomes or other types extracellular vesicles, directly from undiluted whole-blood samples in an automated fashion. Automated exosome isolation enables biohazard containment, short processing time, reproducible results with little human intervention, and convenient integration downstream analysis units. Our method integrating acoustics microfluidics leads to the high purity yield. With its...
Significance The separation and analysis of circulating tumor cells (CTCs) provides physicians a minimally invasive way to monitor the response cancer patients various treatments. Among existing cell-separation methods, acoustic-based approaches provide significant potential preserve phenotypic genotypic characteristics sorted cells, owing their safe, label-free, contactless nature. In this work, we report development an device that successfully demonstrates isolation rare CTCs from clinical...
Rapid and homogeneous mixing inside a microfluidic channel is demonstrated via the acoustic streaming phenomenon induced by oscillation of sidewall sharp-edges. By optimizing design sharp-edges, excellent performance fast speed can be achieved in simple device, making our sharp-edge-based micromixer promising candidate for wide variety applications.
Acoustic tweezers have recently raised great interest across many fields including biology, chemistry, engineering, and medicine, as they can perform contactless, label-free, biocompatible, precise manipulation of particles cells. Here, we present wave number-spiral acoustic tweezers, which are capable dynamically reshaping surface (SAW) wavefields to various pressure distributions facilitate dynamic programmable particle/cell manipulation. SAWs propagating in multiple directions be...
For decades, scientists have pursued the goal of performing automated reactions in a compact fluid processor with minimal human intervention. Most advanced fluidic handling technologies (e.g., microfluidic chips and micro-well plates) lack rewritability, associated benefits multi-path routing re-programmability, due to surface-adsorption-induced contamination on contacting structures. This limits their processing speed complexity reaction test matrices. We present contactless droplet...
A highly reliable, programmable, and cost-effective acoustofluidic pump is demonstrated utilizing the acoustic streaming effects generated by oscillation of tilted sharp-edge structures.
Perturbation approach is utilized to study the acoustic streaming phenomenon induced by oscillation of sidewall sharp-edges.
Abstract The study of circulating tumor cells (CTCs) offers pathways to develop new diagnostic and prognostic biomarkers that benefit cancer treatments. In order fully exploit interpret the information provided by CTCs, development a platform is reported integrates acoustics microfluidics isolate rare CTCs from peripheral blood in high throughput while preserving their structural, biological, functional integrity. Cancer are first isolated leukocytes with 7.5 mL h −1 , achieving recovery...
Patterning of nanowires in a controllable, tunable manner is important for the fabrication functional nanodevices. Here we present simple approach nanowire patterning using standing surface acoustic waves (SSAW). This technique allows construction large-scale arrays with well-controlled geometry and spacing within 5 s. In this approach, SSAWs were generated by interdigital transducers, which induced periodic alternating current (ac) electric field on piezoelectric substrate consequently...
We developed a standing surface acoustic wave (SSAW)-based cell sorting device. The throughput of our device has been significantly improved by using focused interdigital transducers (FIDTs) as SSAW generator.
Recently, acoustically oscillated sharp-edges have been utilized to achieve rapid and homogeneous mixing in microchannels. Here, we present a numerical model investigate acoustic inside sharp-edge-based micromixer the presence of background flow. We extend our previously reported include phenomena by using perturbation analysis Generalized Lagrangian Mean (GLM) theory conjunction with convection-diffusion equation. divide flow variables into zeroth-order, first-order, second-order variables....
A microfluidic cytometer is demonstrated<italic>via</italic>the standing surface acoustic wave (SSAW)-based 3D cell focusing. This SSAW-based flow allows precise cytometry with the advantages of sheathless focusing, bio-compatibility, simple and compact device, significantly reduced biohazards.
A polydimethylsiloxane microchannel featuring sidewall sharp-edge structures and bare channels, a piezoelement transducer is attached to thin glass slide. When an external acoustic field applied the microchannel, oscillation of slide generate streaming flows which in turn rotate single cells C. elegans in-plane out-of-plane. As service our authors readers, this journal provides supporting information supplied by authors. Such materials are peer reviewed may be re-organized for online...
In this work, we develop an in situ method to grow highly controllable, sensitive, three-dimensional (3D) surface-enhanced Raman scattering (SERS) substrates via optothermal effect within microfluidic devices. Implementing approach, fabricate SERS composed of Ag@ZnO structures at prescribed locations inside channels, sites which current fabrication has been arduous. Conveniently, properties the 3D nanostructures such as length, packing density, and coverage can also be adjusted by tuning...
Microfluidic fluorescence-activated cell sorters (μFACS) have attracted considerable interest because of their ability to identify and separate cells in inexpensive biosafe ways. Here a high-performance μFACS is presented by integrating standing surface acoustic wave (SSAW)-based, 3D cell-focusing unit, an in-plane fluorescent detection SSAW-based cell-deflection unit on single chip. Without using sheath flow or precise rate control, the technique can focus into file at designated position....
Extracellular vesicles and lipoprotein contents are separated based on their acoustic constrast factors.
A disposable acoustofluidic platform was developed for nano/microparticle separation with high versatility, precision, and biocompatibility.
Acoustic-based techniques can manipulate particles in a label-free, contact-free, and biocompatible manner. However, most previous work acoustic manipulation has been constrained by axisymmetric patterns of pressure nodes antinodes. Acoustic holography is an emerging technique that offers the potential to generate arbitrary distributions which be applied particle with higher degrees freedom. since current rely on radiation forces, decrease dramatically when target size decreases, they have...
By exploiting surface acoustic waves and a coupling layer technique, cells are patterned within photosensitive hydrogel fiber to mimic physiological cell arrangement in tissues. The aligned cell–polymer matrix is polymerized with short exposure UV light the extracted. These fibers manipulated into simple complex architectures, demonstrating feasibility for tissue-engineering applications.
An acoustofluidic mixer which can achieve thorough mixing across a wide range of flow rates.
Controllable, precise, and stable rotational manipulation of model organisms is valuable in many biomedical, bioengineering, biophysics applications. We present an acoustofluidic chip capable rotating Caenorhabditis elegans (C. elegans) both static continuous flow a controllable manner. Rotational was achieved by exposing C. to surface acoustic wave (SAW) field that generated vortex distribution inside microchannel. By selectively activating interdigital transducers, we bidirectional...