Myocardial ischemia‑reperfusion (I/R) injury may lead to dysfunction of signaling pathways related cell apoptosis, inflammation, oxidative stress, and mitochondrial damage. The present study investigated the defensive effect liriodendrin, as a natural product isolated from Linaria vulgaris, on reperfusion in rats underlying mechanisms involved this process. An vivo rat model I/R constructed by ligation left anterior descending artery, well an vitro using H9C2 cells under hypoxic conditions,...

Background Inflammation in epicardial adipose tissue (EAT) may contribute to coronary atherosclerosis. Myocardial bridge is a congenital anomaly which the left anterior descending artery takes "tunneled" course under of myocardium: while atherosclerosis develops proximal artery, bridged portion spared, highlighting possibility that geographic separation from inflamed EAT protective. We tested hypothesis inflammation was related by comparing and depots individuals with myocardial varying...

BACKGROUND Wastewater discharged from tannery industries is highly complex, concentrated, and toxic. In view of the varying nature wastewater numerous small in China, there a need for efficient treatment processes that are simple to operate have low/reasonable construction operating costs. RESULTS An integrated process upflow anaerobic sludge bed (UASB) – sequencing batch reactor (SBR) electrochemical oxidation (EO) biological aerated filter (BAF) was developed at lab scale with an initial...

Abstract Background The aim of the study is to observe anti‐inflammatory and antioxidative stress effects metformin on bleomycin (BLM)‐induced pulmonary fibrosis in mice. Methods Mice with BLM‐induced were treated pirfenidone, metformin, pirfenidone plus NADPH oxidase 4 (NOX4) inhibitor diphenyleneiodonium chloride (DPI). Pathological changes hydroxyproline (HPO) levels examined lung tissue mice fibrosis. Superoxide dismutase (SOD) activity malonaldehyde (MDA) determined. Results Compared...

Abstract Background Dysregulation of the proliferation and migration vascular smooth muscle cells (VSMCs) is a crucial cause atherosclerosis. MiR-637 exerts an antiproliferative effect on multiple human cells. Its impact atherosclerosis remains largely unexplored. Methods Real-time PCR was used to determine miR-637 expression in samples from patients animal models. VSMC dysfunction models (induced by ox-LDL) also measured. The VSMCs were respectively tested using CCK-8 Transwell assays,...

Vascular calcification is related to many diseases. Iron has a certain relationship with endothelial cells and vascular calcification. The purpose of this study was assess the effect iron overload on cell mechanisms through experiments.Human umbilical vein were treated different concentrations FeSO4 (50, 100, 150, 200 µM), deferoxamine (DFO) ferrostatin. Alkaline phosphatase activity, malondialdehyde (MDA) level, reactive oxygen species (ROS) lipid superoxidation after treatment assessed....

Sepsis is a life‑threatening organ dysfunction caused by dysregulated host response to infection, and leading cause of mortality worldwide. Myocardial associated with poor prognosis in patients sepsis contributes high risk mortality. However, the pathophysiological mechanisms underlying sepsis‑induced myocardial are not completely understood. The aim present study was investigate role toll‑like receptor 4 (TLR4)/c‑Jun N‑terminal kinase (JNK) signaling pro‑inflammatory cytokine expression...

Hyperlipidemia is a risk factor for various cardiovascular and metabolic disorders. And it tightly related to chronic inflammation. Interleukin-38 (IL-38) represents new member of anti-inflammatory cytokines. Thus we studied the important role IL-38 in hyperlipidemia development treatment.The mRNA level PBMCs (peripheral blood mononuclear cells) serum levels patients healthy controls were measured by real-time polymerase chain reaction (RT-PCR) enzyme-linked immunoassay (ELISA). The further...

Atherosclerosis is a severe vascular disorder causing myocardial infarction, stroke, and gangrene. Circular RNA Testis-expressed 14 (hsa_circ_0107197, CircTEX14) newly discovered circRNA that may have critical role in the pathogenesis of atherosclerosis. Here, we aimed to further explore exact TEX14 cardiovascular system. Serum samples atherosclerosis patients (n = 48) healthy volunteers were collected assess circTEX14 expressions. Quantitative reverse transcription-PCR (qRT-PCR), cell...

Abstract Vascular calcification (VC) has been associated with a risk of cardiovascular diseases. Iron may play critical role in progressive VC. Therefore, we investigated the effects iron overload on aorta rats. A rat model was established by intraperitoneal injection Iron-Dextran. The levels iron, calcium, and ALP activity were detected. Von Kossa staining Perl’s conducted. expression metabolism-related related factors examined aortic tissue results showed serum increased induced excessive...

To investigate the effect of microRNA-509-3p (miR-509-3p) on apoptosis atherosclerotic vascular endothelial cells.Mouse aortic cells (MAECs) were divided into normal control group, oxidized low-density lipoprotein (ox-LDL) miR-509-3p overexpression inhibitor + ox-LDL and group. MAEC induced with 100 mg/L for 24 hours, then transfected overexpression/inhibitor corresponding 48 hours. The expression in MAECs exposed to was detected using real-time fluorescence quantitative polymerase chain...

To explore the relationship between serum levels of osteoprotein (OPG), soluble nuclear factor-κB receptor activator ligand (sRANKL), inflammatory factors and coronary heart disease (CHD) its severity.The patients who underwent angiography (CAG) due to chest pain admitted department cardiology Tianjin Chest Hospital from April 2017 December 2018 were enrolled, they divided into CHD group non-CHD according CAG results. The gender, age, history hypertension, smoking history, diabetes,...

Symbiotic protists play important roles in the wood digestion of lower termites. Previous studies showed that termites generally possess host-specific flagellate communities. The genus Reticulitermes is particularly interesting because its unique assemblage gut flagellates bears evidence for transfaunation. fauna species Japan, Europe, and North America had been investigated, but data on China are scarce. For first time, we analyzed phylogeny hindgut five China. A total 22 protist phylotypes...

The aim of this study was to test the preventive effects nano liposomes against contrast-induced nephropathy (CIN) in New Zealand rabbits. Sixty rabbits were randomly divided into four groups, with 15 each group: control group, contrast hydration group and liposome group. Serum creatinine (Scr) Blood Urea Nitrogen (BUN) measured before after injection agent iopromide. Oxidative stress markers, such as superoxide dismutase (SOD) malondialdehyde (MDA), apoptosis Bcl2-Associated X (Bax) B-cell...

Coronary heart disease (CHD) is a complex metabolic syndrome, resulting in atherosclerotic lesions one or more coronary arteries. CHD associated with high morbidity and mortality worldwide. artery bypass grafting (CABG) the gold standard for invasive treatment of severe CHD, especially triple-vessel left main disease. Saphenous veins (SVs) are typically used CABG because their sufficient length convenience, but SV graft (SVG) failure common finding patients. Approximately 20% SVGs fail...

Liriodendrin is a therapeutic constituent of sargentgloryvine stem which famous Chinese traditional medicine. Previous studies have suggested liriodendrin could inhibit different pathways to treat inflammation in lung and intestinal tract. But whether it can myocardial infarction (MI) unknown. We investigated the protective effect on acute MI rats explored specific mechanisms expand use this medicine.The were randomized into sham group (sham operation), control (ligation left anterior...

BACKGROUND:Impaired heart function induced by myocardial infarction is a leading cause of chronic failure (HF). This study aimed to investigate the effects and mechanism noninvasive positive-pressure ventilation (NIPPV) in rat model HF due infarction. MATERIAL AND METHODS:To explore therapeutic effect NIPPV on acute infarction-induced HF, we established ligating anterior descending branch left coronary artery confirmed ultrasonic cardiography brain natriuretic peptide 45 detection....

Mouse models of sepsis-induced myocardial injury by intraperitoneal injection lipopolysaccharide (LPS) was established in order to provide a reliable method for the research pathogenesis injury.According random number table, total 150 male C57BL/6 mice were divided into five groups: NC group, sham and LPS 10, 12, 15 mg/kg groups, with 30 each group. Septic induced mice; group injected equal 0.9% saline; while there no treatment Fifteen used observe general status before after or saline...

To explore the polymorphisms of T149C and T950C gene in osteoprotectin (OPG) promoter sites levels serum OPG soluble nuclear factor-ΚB receptor activator ligand (sRANKL) incidence coronary heart disease (CHD).528 patients Tianjin suspected CHD underwent angiography (CAG) who admitted to department cardiology Chest Hospital from April 2017 December 2018 were enrolled. According CAG results, they divided into two groups: group (n = 302) non-CHD 226). The gender, age, history hypertension,...

The purpose of the research was to investigate preventive effect nanoliposomes on contrast-induced nephropathy (CIN) in New Zealand rabbits and provide a theoretical basis for clinically effective prevention treatment CIN development new contrast agents.A total 48 were divided into four groups randomly, there 12 eacj group: (I) control group; (II) (III) hydration (IV) nanoliposome group. changes serum creatinine (SCr) blood urea nitrogen (BUN) messured before after injection iopromide....