A5079478827- Cutaneous Melanoma Detection and Management
- Advancements in Transdermal Drug Delivery
- Skin Protection and Aging
- Dermatology and Skin Diseases
- Molecular Biology Techniques and Applications
- Enzyme Production and Characterization
- Photoacoustic and Ultrasonic Imaging
- Gold and Silver Nanoparticles Synthesis and Applications
- Nonmelanoma Skin Cancer Studies
- Melanoma and MAPK Pathways
- Identification and Quantification in Food
- Wood and Agarwood Research
- Cell Image Analysis Techniques
- Advanced Fluorescence Microscopy Techniques
- Nanoplatforms for cancer theranostics
- Microbial Metabolism and Applications
- Cutaneous lymphoproliferative disorders research
- Spectroscopy Techniques in Biomedical and Chemical Research
- Plant biochemistry and biosynthesis
- Hepatitis B Virus Studies
- Optical Imaging and Spectroscopy Techniques
- Clostridium difficile and Clostridium perfringens research
- Biofuel production and bioconversion
- Nail Diseases and Treatments
- Extracellular vesicles in disease
The University of Queensland
2013-2022
Guangzhou University of Chinese Medicine
2015-2019
University of South Australia
2019
Blaze Bioscience (United States)
2019
Texas Plastic Surgery
2019
Princess Alexandra Hospital
2011-2017
Translational Research Institute
2013-2017
Wuxi People's Hospital
2015
Nanjing Medical University
2015
Sichuan Agricultural University
2014
Monitoring targeted therapy in real time for cancer patients could provide vital information about the development of drug resistance and improve therapeutic outcomes. Extracellular vesicles (EVs) have recently emerged as a promising biomarker, EV phenotyping shows high potential monitoring treatment responses. Here, we demonstrate feasibility patient responses based on plasma phenotypic evolution using multiplex phenotype analyzer chip (EPAC). EPAC incorporates nanomixing-enhanced microchip...
Fluorescence-guided surgery (FGS) can improve extent of resection in gliomas. Tozuleristide (BLZ-100), a near-infrared imaging agent composed the peptide chlorotoxin and fluorophore indocyanine green, is candidate molecule for FGS glioma other tumor types.To perform phase 1 dose-escalation study to characterize safety, pharmacokinetics, fluorescence tozuleristide adults with suspected glioma.Patients received single intravenous dose 3 29 h before surgery. Fluorescence images cavity Situ...
Abstract Precise detection of early melanomas is essential as the stage disease guides treatment options. One growing field that may facilitate advancement melanoma detection, achieved through profiling serum extracellular vesicles (EVs) using sensitive nanotechnology. As a proof principle, platform combines microfluidic device and surface‐enhanced Raman spectroscopy (SERS), expression profiles 4 protein biomarkers in EVs (termed “EV SERS signatures”) derived from 20 patients (including situ...
Peptides and proteins play an important role in skin health well-being. They are also found to contribute aging melanogenesis. Microneedles have been shown substantially enhance penetration may offer effective means of peptide delivery enhancement. The aim this investigation was assess the influence microneedles on peptides using fluorescence imaging determine distribution. In particular effect chain length (3, 4, 5 amino acid length) passive MN facilitated investigated. Confocal laser...
Abstract Epithelial–mesenchymal transition (EMT) is a primary mechanism for cancer metastasis. Detecting the activation of EMT can potentially convey signs metastasis to guide treatment management and improve patient survival. One classic signatures characterized by dynamic changes in cellular expression levels E‐cadherin N‐cadherin, whose soluble active fragments have recently been reported be biomarkers diagnosis prognosis. Herein, microfluidic immunoassay (termed “SERS immunoassay”) based...
Immune checkpoint blockade (ICB) therapy has achieved remarkable success in many cancers including melanoma. However, ICB benefits only a small proportion of patients and produces severe side effects for some patients. Thus, there is an urgent need to identify who are more likely respond improve outcomes minimize effects. To predict responses, we design surface-enhanced Raman scattering (SERS) assay multiplex profiling circulating tumor cells (CTCs) under basal interferon-γ (IFN-γ)...
Identification of appropriate reference genes (RGs) is critical to accurate data interpretation in quantitative real-time PCR (qPCR) experiments. In this study, we have utilised next generation RNA sequencing (RNA-seq) analyse the transcriptome a panel non-melanoma skin cancer lesions, identifying that are consistently expressed across all samples. Genes encoding ribosomal proteins were amongst most stable dataset. Validation RNA-seq was examined using qPCR confirm suitability set highly for...
Epithelial to mesenchymal transition (EMT) results in the genesis of circulating tumor cells (CTCs) from sites and promotes metastatic capability CTCs circulation. In this study, we develop a multiplex surface-enhanced Raman scattering nanotechnology for comprehensive characterization EMT-associated phenotypes CTCs, monitor cancer metastasis. We observe downregulation CTC marker (EpCAM) epithelial (E-cadherin), as well upregulation (N-cadherin) stem cell (ABCB5) during transforming growth...
We describe the development of a sub-millimetre skin punch biopsy device for painless and suture-free sampling molecular diagnosis research. Conventional biopsies range from 2-4 mm in diameter. Local anaesthesia is required sutures are usually used to close wound. Our microbiopsy 0.50 wide 0.20 thick. The fabricated three stacked medical grade stainless steel plates tapered point contains chamber within centre plate collect sample. observed that application this resulted 0.21 ± 0.04 puncture...
elanocytes, like many other human cells, express the BRAF gene.However, mutation of in melanocytes occurs at high frequency melanocytic proliferations such as nevi (70%-82%) and melanomas (50%-60%). 1,2Mutation results a defect mitogen-activated protein kinase (MAPK) pathway causing oncogenic proliferation avoidance apoptosis. 3Most frequently, mutations occur V600E position (74%-90%), next most common V600K (16%-29%) 4 ; together, these sites account for 95% all mutations.Initial...
Background Ablative fractional laser (AFXL) is an acknowledged technique to increase uptake of topical agents in skin. Micro thermal ablation zones (MAZs) consist ablated vertical channels surrounded by a coagulation zone (CZ). Laser scanning confocal microscopy (LSCM) images individual MAZs at 733 nm (reflectance (RCM)). Further, LSCM can image sodium fluorescein (NaF) fluorescence with 488 excitation (fluorescence microcopy (FCM)), small hydrophilic test molecule (370 MW, log P ‐1.52),...
Abstract Actinic keratoses (AK) arise in severely photo-damaged skin and can progress to squamous cell carcinomas (SCC). AK SCC are common Caucasian populations, immunosuppressed individuals have a markedly higher risk of developing SCC. An overabundance Staphylococcus aureus has been reported lesions immunocompetent individuals, however, the AK/SCC microbiome cohorts not investigated. Here, microbial profile bacterial load AK, control swabs from 32 organ transplant recipients were...
<ns4:p>We describe the development of a sub-millimetre skin punch biopsy device for painless and suture-free sampling molecular diagnosis research. Conventional biopsies range from 2-4 mm in diameter. Local anaesthesia is required sutures are usually used to close wound. Our microbiopsy 0.50 wide 0.20 thick. The fabricated three stacked medical grade stainless steel plates tapered point contains chamber within centre plate collect sample. We observed that application this resulted 0.21 ±...
The nanotechnology field is growing at an unprecedented rate. This resulting in significant benefits skin care products and formulations. Likewise, imaging technology also advancing. convergence of these fields offers a unique opportunity to observe quantify the interactions nanoparticles within cosmetic More importantly, holds tremendous promise for understanding how formulated interact with our skin. Imaging technologies can be broken into two major groups that include those require...
Abstract It has been shown that gene mutations which drive the development of malignant melanoma ( MM ) in humans also lead to emergence when engineered mice. However, little attention paid clinical and histopathological features melanocytic lesions their natural history a given mouse model. This knowledge is crucial enable us understand how influence initiation evolution lesions, and/or for use mice as preclinical model test specific treatments. We recently reported proliferations along...
Delivery of therapeutics into skin is hindered by the epidermal barriers. To overcome these barriers for treatment diseases, a cutaneous delivery method capable field using silica-elongated microparticles developed. The are massaged 3D-printed microtextured applicator resulting in significant field-directed drug enhancement. As service to our authors and readers, this journal provides supporting information supplied authors. Such materials peer reviewed may be re-organized online delivery,...
The BRAF V600E mutation accounts for the majority of mutations found in cutaneous melanoma and is also commonly nevi. We used dermoscopy-targeted sampling a microbiopsy device coupled with DNA sequence analysis to highlight heterogeneity within multicomponent melanocytic proliferation. This technique demonstrates prospect vivo application clinical setting.A man his 50s Fitzpatrick skin type II presented an irregularly pigmented lesion on back that met melanoma-specific dermoscopic criteria,...
Inflammatory skin conditions are the 4th leading cause of non-fatal health burden in general population worldwide. The diagnosis lesions due to systemic drug reactions, viral or bacterial exanthems, patients with psoriasis, atopic dermatitis contact is often difficult and relies heavily upon conventional histopathologic examination. Conversely, it widely accepted that cutaneous profile inflammatory markers, 'inflammatory signature', differentially expressed various conditions. In this pilot...