A5046921613- CRISPR and Genetic Engineering
- Air Quality and Health Impacts
- Genetic Neurodegenerative Diseases
- Pluripotent Stem Cells Research
- Noise Effects and Management
- Air Quality Monitoring and Forecasting
- Neurological disorders and treatments
- RNA and protein synthesis mechanisms
- Neuroinflammation and Neurodegeneration Mechanisms
- Genetics, Aging, and Longevity in Model Organisms
- Social and Educational Sciences
- Neuroscience and Neuropharmacology Research
- Indoor Air Quality and Microbial Exposure
- Mitochondrial Function and Pathology
- Retinal Development and Disorders
- S100 Proteins and Annexins
- Traumatic Brain Injury Research
- Inhalation and Respiratory Drug Delivery
- Traumatic Brain Injury and Neurovascular Disturbances
- Forest ecology and management
- Virus-based gene therapy research
- Health, Environment, Cognitive Aging
- Boron Compounds in Chemistry
- Signaling Pathways in Disease
- Urinary Tract Infections Management
AstraZeneca (Sweden)
2018-2025
Karolinska Institutet
1990-2018
Elekta (Sweden)
2010
Danderyds sjukhus
2004-2009
Stockholm County Council
2006-2007
Umeå University
2007
Karolinska University Hospital
1997-2000
Abstract Genome editing, specifically CRISPR/Cas9 technology, has revolutionized biomedical research and offers potential cures for genetic diseases. Despite rapid progress, low efficiency of targeted DNA integration generation unintended mutations represent major limitations genome editing applications caused by the interplay with double-strand break repair pathways. To address this, we conduct a large-scale compound library screen to identify targets enhancing insertions. Our study reveals...
The mutation patterns at Cas9 targeted sites contain unique information regarding the nuclease activity and repair mechanisms in mammalian cells. However, analytical framework for extracting such are lacking. Here, we present a novel computational platform called Rational InDel Meta-Analysis (RIMA) that enables an in-depth comprehensive analysis of Cas9-induced genetic alterations, especially InDels mutations. RIMA can be used to quantitate contribution classical microhomology-mediated end...
In vivo studies of human brain cellular function face challenging ethical and practical difficulties. Animal models are typically used but display distinct differences. One specific example is astrocytes, recently recognized for contribution to neurological diseases a link the genetic risk factor apolipoprotein E (APOE). Current astrocytic in vitro questioned lack biological characterization. Here, we report induced pluripotent stem cell (hiPSC)-derived astroglia (NES-Astro) developed under...
Abstract The CRISPR-Cas9 system has increased the speed and precision of genetic editing in cells animals. However, model generation for drug development is still expensive time-consuming, demanding more target flexibility faster turnaround times with high reproducibility. a tightly controlled ObLiGaRe doxycycline inducible SpCas9 (ODInCas9) transgene its use targeted results functional integration into both human mouse culminating ODInCas9 mouse. Genomic can be performed various tissue...
In vivo CRISPR gene editing holds enormous potential for various diseases. Ideally, delivery should be cell type-specific and time-restricted optimal efficacy safety, but customizable methods are lacking. Here we develop a cell-tropism programmable CRISPR-Cas9 ribonucleoprotein system (RIDE) based on virus-like particles. The efficiency of RIDE was comparable to that adeno-associated virus lentiviral vectors higher than lipid nanoparticles. could readily reprogrammed target dendritic cells,...
Human pulmonary retention of 35 nm 99mTc-labeled carbonaceous particles, produced with a modified Technegas generator, was followed for 24 h using gamma camera imaging technique. Nine healthy subjects and four asthmatics inhaled the test particles. Particle labeling stability tested in vitro during 48 h. We also measured vivo leaching blood urine One additional subject exposed to particles unstable labeling. There were no significant differences between asthmatic any parameters studied....
The aim of this study was to explore the relationship between proteins S100B and S100A1B symptoms signs cognitive impairment for 3 months after mild traumatic brain injury (MTBI).Serum concentrations were examined in a prospective cohort patients with MTBI Glasgow Coma Scale score 14 or 15. Cognitive performance assessed by repeated computerized neuropsychological testing an extended test. Symptoms using Rivermead Post-Concussion Questionnaire.Concentrations above cut-off 31% 48%...
Traffic-related air pollution is associated with adverse respiratory effects. The aim of the present study was to investigate whether exposure in a road tunnel causes airway inflammatory and blood coagulation responses. A total 16 healthy subjects underwent bronchoscopy bronchial mucosal biopsies bronchoalveolar lavage (BAL) on two occasions, random order: once at 14 h after 2-h busy tunnel, control day exposed urban during normal activities. Peripheral sampled prior bronchoscopy. exposures...
Prokaryotic restriction enzymes, recombinases and Cas proteins are powerful DNA engineering genome editing tools. However, in many primary cell types, the efficiency of remains low, impeding development gene- cell-based therapeutic applications. A safe strategy for robust efficient enrichment precisely genetically engineered cells is urgently required. Here, we screen mutations receptor Diphtheria Toxin (DT) which protect human from DT. Selection with an edited DT variant enriches...
This study examined the first participants who registered for Huntington's disease predictive testing program 1990–1995 in Stockholm, Sweden. A psychosocial investigation was performed to evaluate potential effects of presymptomatic testing. The results showed no significant differences between 13 genecarriers and 21 noncarriers pretest attitudes, expectations, general well-being, life satisfaction lifestyle, need support, estimated sense well-being or degree health. However, both groups...
To examine the diagnostic value of S100 in mild traumatic brain injury (MTBI).Prospective cohort study.S100B, S100A1B and S100BB concentrations were examined sera from patients with MTBI an arrival Glasgow Coma Scale score 15 or 14, orthopaedic injuries non-injured subjects.Mean values proportions subjects above cut-off limits for S100B significantly higher each trauma group than controls, but only when compared controls injuries. Using a 97.5 percentile limit, sensitivity vs was 61% (95%...
The primary focus of this study was to examine whether there is early neuropsychological impairment in presymptomatic Huntington's disease (HD). A broad assessment battery administered 24 asymptomatic gene carriers (HD+) and 31 noncarriers (HD-). revealed inferior cognitive functioning as compared with the memory executive functions. When were assigned 2 groups based on predicted years onset (with 15 over being HD+ late under near), near group performed significantly worse than all domains...
Environmental particle exposure, often estimated as the particulate mass of particles with a diameter <10 μm, <2.5 μm or <1 (PM 10 , PM 2.5 1 ), is known to have negative impact on health population. Little about how size and origin influence effects. We previously shown that exposure road tunnel environment causes cellular inflammatory response in airways healthy individuals. In present study, our aim was investigate potential airway effects from subway environment. 20 volunteers...
Abstract We used single‐cell RNA sequencing (seq) on several human induced pluripotent stem (iPS) cell‐derived neural cell (NSC) lines and one fetal brain‐derived NSC line to study inherent type heterogeneity at proliferating stage uncovered predisposed presence of neurogenic gliogenic progenitors. observed in progenitors that differed between the iPS fetal‐derived line, we also differences spontaneous differentiation potential for inhibitory excitatory neurons line. In addition, using a...
Thirty girls, studied in 1961 after 2.5 years of intensive swimtraining, were the subject a follow-up for ten years. When last examined, seven and original study, all girls had given up swimtraining. The increased values vital capacity observed remained unchanged, but residual volume, functional total lung showed small increases even corrections body growth. Such are, however, normal these Heart volume which was high originally, found to be lower later, although mean still higher than...
<h3>Background:</h3> Being male constitutes a risk factor for early death, and this may be connected to concepts of masculinity. The objective the present study was investigate association between masculinity rank during late adolescence mortality patterns in cohort 49 321 Swedish men tested compulsory military training 1969. <h3>Methods:</h3> measure consisted five-grade ranking from conscription information that comprised leisure interests occupational preferences. Information on all-cause...
Huntington's disease (HD) is a neurodegenerative disorder initially affecting the basal ganglia and especially head of caudate nucleus. Neuropsychological research has indicated that olfactory dysfunction may appear early in HD, prior to onset significant motor or cognitive dysfunction. The aim this study was examine whether asymptomatic carriers Huntington mutation also exhibit To address issue we presented an extensive test battery comprising tasks assessing sensitivity, intensity...
A predictive testing program for Huntington disease has been available in Stockholm, Sweden since October 1990. Psychosocial assessments were performed throughout the to evaluate impact of risk situation itself and effect testing, identify those individuals who most vulnerable severe stress anxiety reactions. All subjects underwent neurological, neuropsychological, psychiatric examinations. Individuals undergoing assessed twice by a genetic counsellor before receiving their results, at 10...
Single-dose trimethoprim treatment of cystitis was compared with 7-day in a randomized double-blind study primary health care. Consecutive female patients (613) symptoms lower urinary tract infection (UTI) and positive bacteriuria screening tests were enrolled. In 502 cases UTI confirmed by urine culture. Follow-up performed twice, after 2–3 5–6 weeks. Short-term efficacy could beevaluated 425 accumulated 344. 82% for single-dose 94% (P < .001). Accumulated 71% 87% therapy Fewer adverse...
Continuous environmental or occupational exposure to airborne particulate pollution is believed be a major hazard for human health. A technique characterize their deposition and clearance from the lungs fundamental understand underlying mechanisms behind negative health effects. In this work, we describe method production follow up of ultrafine carbon particles labeled with radioactive 111Indium (111In). The physicochemical biological properties aerosol are described in terms particle size...