A5101609649- Animal Genetics and Reproduction
- RNA Research and Splicing
- Sperm and Testicular Function
- Immune Response and Inflammation
- Chromosomal and Genetic Variations
- Animal Nutrition and Physiology
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Cellular transport and secretion
- RNA and protein synthesis mechanisms
- Immune Cell Function and Interaction
- Animal Virus Infections Studies
- Salmonella and Campylobacter epidemiology
- Autism Spectrum Disorder Research
- NF-κB Signaling Pathways
- MicroRNA in disease regulation
- Liver Disease Diagnosis and Treatment
- Circular RNAs in diseases
- Epigenetics and DNA Methylation
- Gut microbiota and health
- Signaling Pathways in Disease
- Analytical Chemistry and Chromatography
- Aquaculture disease management and microbiota
- Genetics and Neurodevelopmental Disorders
- Genomics and Chromatin Dynamics
- Esophageal Cancer Research and Treatment
Zhejiang Lab
2024
Jinzhou Kangning Hospital
2023-2024
Wenzhou Medical University
2023-2024
Jiangsu Province Hospital
2024
Nanjing Medical University
2024
Second Affiliated Hospital & Yuying Children's Hospital of Wenzhou Medical University
2023
University of Michigan
2023
Yangzhou University
2014-2018
Changshu Institute of Technology
2018
Abstract As the most prevalent neurodevelopmental disorders in children, autism spectrum (ASD) are characterized by deficits language development, social interaction, and repetitive behaviors or inflexible interests. Contactin associated protein like 2 (CNTNAP2) , encoding a single transmembrane with 1331 amino acid residues, is widely validated ASD-susceptible gene. Cntnap2 -deficient mice also show core autism-relevant behaviors, including behavior. However, cellular mechanisms underlying...
Abstract Mutations in the Contactin-associated protein-like 2 ( CNTNAP2 ) gene are associated with autism spectrum disorder (ASD), and ectodomain shedding of protein plays a role its function. However, key enzymes involved C-terminal cleavage remain largely unknown, effect ASD-associated mutations on this process ASD pathogenesis elusive. In report we showed that undergoes sequential cleavages by furin, ADAM10/17-dependent α-secretase presenilin-dependent γ-secretase. We identified sites...
Spermatogenesis is a complex process that involves many elements. However, until now, little known at the molecular level about spermatogenesis in poultry. Here we investigated microRNAs and their target genes may be involved germ cell development spermatogonial chicken. We used next-generation sequencing to analyze miRNA expression profiles three types of germline cells: primordial cells (PGCs), stem (SSCs), spermatogonia (Sp) during early stage spermatogenesis. After validated candidate...
Chicken reticuloendotheliosis virus (REV) causes the atrophy of immune organs and immuno-suppression. The pathogenic mechanisms REV are poorly understood. aim this study was to use RNA sequencing analyse effect on immunity cell proliferation in chicken lymphocytes from peripheral blood vitro. Overall, 2977 differentially expressed genes (DEGs) were examined cells between infected with or no; 56 DEGs related 130 identified. MTT, Q-PCR FCM indicated that reduced number by inhibiting transition...
IMP (inosine 5'-monophosphate) is a compound that enhances the flavor of poultry meat. has become new breeding trait to improve meat quality. We tried identify several potential regulatory genes, and construct their predicted relationships. Time series gene expression profiles thigh muscle tissues Rugao chicken, famous indigenous breed in China, were performed for analysis genes are co-expressed or correlated with concentration IMP. found 15 crucial co-expression which Hspa2, Pten, Gabpa,...
Piwi-interacting RNAs (piRNAs) play a key role in spermatogenesis. Here, we describe the piRNAs profiling of primordial germ cells (PGCs), spermatogonial stem (SSCs), and spermatogonium (Sp) during early-stage spermatogenesis chicken. We obtained 31,361,989 reads from PGCs, 31,757,666 SSCs, 46,448,327 Sp cells. The length distribution three samples showed peaks at 33 nt. resulting genes were subsequently annotated against Gene Ontology (GO) database. Five (RPL7A, HSPA8, Pum1, CPXM2, PRKCA)...
Salmonella enterica Enteritidis ( S. ) and Pullorum pullorum are regarded as a threat to poultry production. This study’s aim is characterize the expression profiles in response three different challenges identify infection-related genes chicken spleen caecum. Groups of Chinese breed Langshan were challenged with either , or poly(I:C). The concentrations cytokines antibodies colonization level caecum liver detected each group at 7 days postinfection. Expression microarray experiments...
Down syndrome (DS) is caused by triplication of human chromosome 21 (HSA21). Although several HSA21 genes have been found to be responsible for aspects DS, whether and how interact with each other poorly understood. DS patients animal models present a number neurological changes, including aberrant connectivity neuronal morphology. Previous studies indicated that amyloid precursor protein (APP) cell adhesion molecule (DSCAM) regulate morphology contribute aberrations in DS. Here, we report...
Spermatogenesis is a complex process. Some studies have shown that Piwi-interacting RNAs (piRNAs) play an important role in spermatogenesis. To verify the evaluate between piRNAs and PIWI proteins chicken its possible spermatogenesis reproductive stem cell proliferation differentiation, we performed immunoprecipitation deep sequencing analyses determined expression profiles of small primordial germ cells (PGCs), spermatogonial (SSCs), spermatogonia (Sa) cells, spermatozoa. Length analysis...
NLRC5 plays an important role in the innate immunity and cellular many species, but regulatory mechanism of expression chickens remains unclear. In this study, a series deletion fragments promoter region were constructed dual-luciferase assay was performed. Then, we detected SNP core its function. Important transcriptional elements predicted identified. Methylation CpG islands measured. The results revealed that two regions -4372 to -3756 -2925 -2265 essential for mRNA which (A/G), located...
Chicken is a main poultry in China. Molecular breeding for disease resistance plays an important role the control of diseases, especially infectious diseases. Choice genes key technology molecular breeding. The major histocompatibility complex (MHC) great interest to scientists its extraordinary polymorphism and close relation with traits against MHC-B haplotype study chicken. traditional chicken commonly defined by serologic reactions erythrocytes majority studies have been conducted...
The P-element induced wimpy testis (Piwi) protein family is responsible for initiating spermatogenesis and maintaining the integrity of germ cells stem cells, but little known regarding its transcriptional regulation in poultry. Here, we characterized methylation status Piwil1 promoter five different spermatogenic cell lines using direct bisulfite pyrosequencing determined that correlates negatively with type-specific expression patterns piwil1. We demonstrated -148 CpG site, which predicted...
BACKGROUND Esophageal cancer is the sixth leading cause of cancer-related death and eighth most common cancer, affecting > 450000 people worldwide. squamous cell carcinoma histological type, whereas esophageal adenoid cystic (EACC) rare. The liver distant metastatic site in cancer. Anal metastasis rare has not been reported clinical practice before. Here, we report anal metastases a patient with EACC after regular chemotherapy surgical resection. CASE SUMMARY A 61-year-old was found to...
Asthenozoospermia puzzled poultry industry and little known about its characteristics. To explore the histological etiology of asthenozoospermia, we measured sperm quality index (SQI) Piwil1 expression in testes three types asthenozoospermia roosters: field, artificial normal. The SQI field roosters was inferior to normal roosters. Further, busulfan could reduce quality. Histological examination showed that spermatids spermatocyte seminiferous tubule stripped layer-by-layer absolute Piwi11...