A5052284995- Blood groups and transfusion
- Erythrocyte Function and Pathophysiology
- Hemoglobinopathies and Related Disorders
- Hepatitis B Virus Studies
- Platelet Disorders and Treatments
- Blood disorders and treatments
- Chronic Lymphocytic Leukemia Research
- Immunodeficiency and Autoimmune Disorders
- Protein Structure and Dynamics
- Parvovirus B19 Infection Studies
- Enzyme Structure and Function
- Blood donation and transfusion practices
- Genetic Syndromes and Imprinting
- Monoclonal and Polyclonal Antibodies Research
- Phagocytosis and Immune Regulation
- Hematopoietic Stem Cell Transplantation
- Colorectal Cancer Treatments and Studies
- Prenatal Screening and Diagnostics
- IL-33, ST2, and ILC Pathways
- Glycosylation and Glycoproteins Research
- Dermatology and Skin Diseases
- Hepatitis C virus research
- Blood transfusion and management
- Medical Research and Practices
- Kruppel-like factors research
Établissement Français du Sang
2018-2024
Inserm
2006-2024
Institut Mondor de Recherche Biomédicale
2017-2024
Université Paris-Est Créteil
2017-2024
Regeneron (United States)
2023-2024
New York Blood Center
2021-2023
Laboratoire de Biologie Moléculaire et Cellulaire des Eucaryotes
2023
Laboratory of Excellence GR-Ex
2015-2021
Groupe de Recherches et d'Echanges Technologiques
2017
Centre Hospitalier de Bretagne Sud
2016
Introduction: Blood group antigens of the RH system (formerly known as “Rhesus”) play an important role in transfusion medicine because severe haemolytic consequences antibodies to these antigens. No crystal structure is available for RhD proteins with its partner RhAG, and precise stoichiometry trimer complex remains unknown. Methods: To analyse their structural properties, trimers formed by and/or RhAG subunits were generated protein modelling molecular dynamics simulations performed....
Continuous variables are expressed as means ± one standard deviation (SD) or medians (MD, [interquartile range]), depending on whether they normally asymmetrically distributed.Categorical numbers (%).For comparison with the largest published delayed hemolytic transfusion reaction (DHTR) series, data in column 2 reprinted from Habibi et al.1 permission.The patients of our who received anti-C5 antibody, had very severe DHTR hyperhemolysis (P-values 3 compare those historical series).*Six not...
Protein structures are highly dynamic macromolecules. This dynamics is often analysed through experimental and/or computational methods only for an isolated or a limited number of proteins. Here, we explore large-scale protein simulation to observe local conformations using different perspectives. We molecular investigate flexibility locally, classical approaches such as RMSf, solvent accessibility, but also innovative entropy. First, focussed on secondary and specifically how β-strand,...
Many RhD variants associated with anti-D formation (partial D) in carriers exposed to the conventional D antigen carry mutations affecting extracellular loop residues. Surprisingly, some transmembrane or intracellular domains, positions not thought likely have a major impact on epitopes.A wild-type Rh trimer (RhD1 RhAG2 ) was modeled by comparative modeling human RhCG structure. Taking conformation, residue accessibility, and position relative lipid bilayer into account, we redefine domains...
Red blood cell (RBC) transfusion is a major therapy for sickle disease (SCD). Patients are at risk of forming antibodies to RBC antigens, which can result in the impossibility find compatible units and cause hemolytic reactions. This retrospective study investigates evolution consumption frequencies, specificities, chronology appearance population patients consistently receiving RH (C, D, E, c, e) K-matched (RBCus) from predominantly European donor population. Over 11-year period Paris area,...
Our objective was to define the pharmacodynamic profile of new dual neutral endopeptidase (NEP)/angiotensin-converting enzyme (ACE) inhibitor AVE7688.We compared effects single oral doses AVE7688 (5 and 25 mg) with those 10 mg ramipril (R10), a selective ACE inhibitor, in placebo-controlled crossover study sodium-depleted normotensive subjects. We also renin-angiotensin system (RAS) blockade induced by high dose an angiotensin II receptor antagonist (300 irbesartan) RAS (150 irbesartan plus...
BACKGROUND Sickle cell disease (SCD) patients undergo multiple red blood (RBC) transfusions and are regularly exposed to low‐prevalence (LP) antigens specific individuals of African descent. This study evaluated the prevalence antibodies against LP in SCD need identify these everyday practice. STUDY DESIGN AND METHODS Plasma from 211 was tested with RBCs expressing following antigens: RH10 (V), RH20 (VS), RH23 (D W ), RH30 (Go a KEL6 (Js MNS6 (He). RESULTS Nine were found eight (3.8%): five...
Abstract Background The basal cell adhesion molecule (BCAM) carries the antigens of Lutheran (LU, ISBT005) system. We report a novel antigen and propose an updated, full‐length 3D model BCAM. Study Design Methods Red blood testing, antibody identification, BCAM genomic DNA sequencing were done by standard methods. Multi‐template homology modeling used structural templates selected for coverage, highest sequence identity, protein domain family. All variants causing loss or gain analyzed...
IDH2 mutations are commonly associated with 1p/19q codeletion in diffuse adult gliomasDiffuse gliomas classified according to the 2016 World Health Organization (WHO) Classification of Tumors Central Nervous System, 1 which combines histological and molecular features.Diagnosis requires assessment isocitrate dehydrogenase genes (IDH1 IDH2), key genetic alterations characterizing favorable outcome. 2 Because IDH1 highly similar enzymes, WHO classification, as most current studies, these into...
Objective: This work proposes a review of the antibodies which have been associated with variant RHD and RHCE alleles, except null alleles. Background: The data on this topic is dispersed in literature. Methods: A articles referenced PubMed abstract books from major conferences was performed. Most published full-length articles, several more reported conference abstracts. anti-D carriers D variants to CE antigens were listed, including low prevalence antigens. alleles for RH10 (V) RH20 (VS)...
Partial D status is a major concern for transfusion and pregnancy, due to the possibility of carriers becoming immunized. When known variant have never been exposed complete D, they are assumed partial based on position amino acid substituted. New approaches predicting immunization risk required. We built three-dimensional (3D) structural model investigate consequences substitutions Amino Acid 223 involved in large number variants.Homology modeling was performed with multiple templates. The...
Intrinsic Disorder Proteins (IDPs) have become a hot topic since their characterisation in the 90s. The data presented this article are related to our research entitled "A structural entropy index analyse local conformations Intrinsically Disordered Proteins" published Journal of Structural Biology [1]. In study, we quantified, for first time, continuum from rigidity flexibility and finally disorder. Non-disordered regions were also highlighted ensemble disordered proteins. This work was...
Abstract Background Scianna (Sc) antigens, seven high and two of low prevalence, are expressed on erythrocyte membrane‐associated protein (ERMAP). We investigated SC ( ERMAP) in individuals who made antibodies to prevalence propose a 3D model for ERMAP precisely localize the residues associated with known antigens. Methods Serological testing DNA sequencing was performed by standard methods. A structural built using multi‐template homology approach. Protein structures representing missense...
Cromer antigens are carried on decay accelerating factor (DAF, CD55), for which the crystal structure is available. We investigated two samples with an unidentified antibody to a high prevalence antigen and evaluate location characteristics of amino acids associated CD55 by 3D modelling.Antigen typing identification were standard methods. was sequenced, variants generated using protein's (1OK3, chain A). Antigen-associated residues intraprotein interactions in (Naccess, Protein Interactions...
<title>Abstract</title> In the case of donor/recipient rhesus (Rh)-incompatibility after allogeneic hematopoietic stem cell transplantation (alloHSCT), transfusion policy in France is to transfuse red blood cells (RBC) donor’s Rh phenotype from day transplantation, leading a risk allo-immunization, either donor or recipient origin. this single-center retrospective study, incidence incompatibility was 7.1% over an eight-year period including 1 012 alloHSCT. Six 58 evaluable patients (10.3%)...