A5019121792- Nanoplatforms for cancer theranostics
- Lung Cancer Diagnosis and Treatment
- Gold and Silver Nanoparticles Synthesis and Applications
- Photodynamic Therapy Research Studies
- Biosensors and Analytical Detection
- Aortic aneurysm repair treatments
- Photoacoustic and Ultrasonic Imaging
- Cancer Research and Treatments
- Nanoparticle-Based Drug Delivery
- Advanced biosensing and bioanalysis techniques
- Spectroscopy Techniques in Biomedical and Chemical Research
- Renal and Vascular Pathologies
- Peripheral Artery Disease Management
- Radiomics and Machine Learning in Medical Imaging
- Extracellular vesicles in disease
- Antimicrobial Peptides and Activities
- Cancer Cells and Metastasis
- Optical Coherence Tomography Applications
- Fetal and Pediatric Neurological Disorders
- Endometrial and Cervical Cancer Treatments
- Advanced Neuroimaging Techniques and Applications
- Antenna Design and Optimization
- Cerebrovascular and Carotid Artery Diseases
- Quantum Dots Synthesis And Properties
- Vascular Procedures and Complications
University of Toronto
2012-2022
Toronto Metropolitan University
2018
St. Michael's Hospital
2018
University Health Network
2008-2016
Princess Margaret Cancer Centre
2014
Ontario Institute for Cancer Research
2012-2014
Toronto General Hospital
2012
University of Waterloo
2007
Eaton (United States)
2005
Multiplexed surface-enhanced Raman scattering (SERS) nanoparticles (NPs) offer the potential for rapid molecular phenotyping of tissues, thereby enabling accurate disease detection as well patient stratification to guide personalized therapies or monitor treatment outcomes. The clinical success diagnostics based on SERS NPs would be facilitated by ability accurately identify tissue biomarkers under time-constrained staining and conditions with a portable device. In vitro, ex vivo in...
A porphyrin-phospholipid conjugate with quenched fluorescence was utilized to serve as both the Raman dye and a stabilizing, biocompatible surface coating agent on gold nanoparticles. Through simple synthesis validation spectroscopy confocal microscopy, we show that this porphyrin-lipid stabilized AuNP is novel SERS probe capable of cellular imaging. To date, first use porphyrin reporter molecule for based
In recent years numerous studies have shown the potential advantages of molecular imaging in vitro and vivo using contrast agents based on surface enhanced Raman scattering (SERS), however low throughput traditional point-scanned methodologies limited their use biological imaging. this work we demonstrate that direct widefield a tunable filter is capable quantitative multiplex SERS vivo, possible with acquisition times which are orders magnitude lower than achievable comparable...
As the possibilities of molecular imaging in personalized medicine evolve rapidly, optical advantages extremely narrow and intense spectral bands makes surface-enhanced Raman scattering (SERS) an appealing candidate for multiplexed recognition targeted biomarkers over other modalities.In this proof-of-concept study, we report wide-field detection lung cancer using multimodal SERS nanoprobes specific to EGF receptor family, both vitro vivo.For first time, demonstrate vivo after topical...
Abstract Early detection and efficient treatment modality of early-stage peripheral lung cancer is essential. Current nonsurgical treatments for show critical limitations associated with various complications, requiring alternative minimally invasive therapeutics. Porphysome nanoparticle-enabled fluorescence-guided transbronchial photothermal therapy (PTT) was developed demonstrated in preclinical animal models. Systemically administered porphysomes accumulated tumors significantly enhanced...
As molecular imaging moves towards lower detection limits, the elimination of endogenous background signals becomes imperative. We present a facile background-suppression technique that specifically segregates signal from surface-enhanced Raman scattering (SERS)-active nanoparticles (NPs) tissue autofluorescence in vivo. SERS NPs have extremely narrow spectral peaks do not overlap significantly with signals. This can be exploited, using specific narrow-band filters, to image picomolar (pM)...
BACKGROUND: Endoluminal optical imaging, or angioscopy, has not seen widespread application during neurointerventional procedures, largely as a result of the poor imaging resolution existing angioscopes. Scanning fiber endoscopes (SFEs) are novel endoscopic platform that allows high-resolution video in an ultraminiature form factor is compatible with currently used distal access endoluminal catheters. OBJECTIVE: To test feasibility and potential utility angioscopy SFE common endovascular...
We report on the design and testing of a prototype widefield surface enhanced Raman scattering (SERS) imaging system based fiber optic bronchoscope using bandpass filters for signal selection. The SERS contrast agents employed consist gold nanoparticles encoded with Raman-active dye made specific lung adenocarcinoma tissue through use an anti-epidermal growth factor receptor (EGFR) antibody. By exploiting extremely narrow spectral peaks we demonstrate facile method background fluorescence...
Abstract Quantum dot (QD) contrast‐enhanced molecular imaging has potential for early cancer detection and image guided treatment, but there is a lack of quantitative contrast data to determine optimum QD administered doses, affecting the feasibility, risk cost such procedures, especially in vivo. Vascular fluorescence performed on nude mice bearing dorsal skinfold window chambers, injected with 4 different solutions emitting visible near infrared. Linear relationships are observed among...
Isolated extremity arterial aneurysms remain a rare entity, and the development of spontaneous arteriovenous fistula from such an aneurysmal segment in young patient should prompt search for underlying genetic predisposition. Endovascular repair or fistulas popliteal artery is appropriate select populations; however, open allows more durable reconstruction both any involved venous segments patients who can tolerate procedure.
To demonstrate the feasibility and potential utility of high-resolution angioscopy during common endovascular interventions.A 3.7-F scanning fiber angioscope was used in 6 Yorkshire pigs to image branch vessel selection, subintimal dissection, wire snaring, stent placement. The introduced a coaxial fashion within standard 6-F guide catheter. A clear field view provided using continuous heparinized saline flush through outer flow rate manually adjusted provide imaging depending on diameter...
The scanning fiber endoscope (SFE) is a novel medical imaging device that has been used in various vascular beds as form of angioscopy, well tracts and duct systems for endoluminal imaging. Owing to its miniaturized form, high resolution, flexibility, it demonstrated success across wide range diagnostic applications.To demonstrate, by performing third ventriculostomy visualizing the cranial nerves brainstem anatomy, that, without modification, SFE can be through transcranial approach...
Intravital microscopy has been demonstrated to be a powerful technique for studying the delivery of contrast or therapeutic agents tumours growing in realistic 3D environment at high resolution. Surface enhanced Raman scattering (SERS)-active nanoparticle provide ability improve <i>in-vivo</i> detection tumour tissue through multiplex their uniquely bright spectral lines. However, most work date carried out <i>in-vitro</i> <i>ex-vivo</i> tissues. Here we present results from confocal dorsal...
We are developing a miniature fiber-optic spectral-detection device and topical-staining protocol to rapidly detect multiplexed surface-enhanced Raman scattering (SERS) nanoparticles (NPs) targeted cell-surface biomarkers in fresh tissues. Ex vivo experiments were performed optimize our strategy for the rapid detection of multiple following brief (5 min) topical application SERS NPs on The simultaneous ratiometric quantification nontargeted allows an unambiguous assessment molecular...