A5087033400- Lung Cancer Diagnosis and Treatment
- Nanoplatforms for cancer theranostics
- Photodynamic Therapy Research Studies
- Tracheal and airway disorders
- Radiopharmaceutical Chemistry and Applications
- Photoacoustic and Ultrasonic Imaging
- Venous Thromboembolism Diagnosis and Management
- Mass Spectrometry Techniques and Applications
- Medical Imaging Techniques and Applications
- Ultrasound and Hyperthermia Applications
- Cancer, Hypoxia, and Metabolism
- Ultrasound in Clinical Applications
- Spectroscopy Techniques in Biomedical and Chemical Research
- Radiomics and Machine Learning in Medical Imaging
- Medical Imaging and Pathology Studies
- Cancer Research and Treatments
- Atrial Fibrillation Management and Outcomes
- Lung Cancer Treatments and Mutations
- Cancer Cells and Metastasis
- Metabolomics and Mass Spectrometry Studies
- Lung Cancer Research Studies
- Airway Management and Intubation Techniques
- Drug Transport and Resistance Mechanisms
- Nanoparticle-Based Drug Delivery
- Glioma Diagnosis and Treatment
Toronto General Hospital
2019-2024
University Health Network
2016-2024
Faculty of 1000 (United States)
2023
Health Net
2020-2023
RELX Group (United States)
2023
Princess Margaret Cancer Centre
2020
Toronto Public Health
2020
University of Toronto
2017-2019
West Virginia University
2017
PhytoCeutica (United States)
2017
Identification of necrosis in tumors is prognostic value treatment planning, as associated with aggressive forms cancer and unfavourable outcomes. To facilitate rapid detection Mass Spectrometry (MS), we report the lipid MS profile necrotic breast Desorption Electrospray Ionization (DESI-MS) imaging validated statistical analysis correlating pathology. This characterized by (1) presence ion m/z 572.48 [Cer(d34:1) + Cl]- which a ceramide absent from viable subregions; (2) absence 391.25...
Deposition of liposomal drugs into solid tumors is a potentially rate-limiting step for drug delivery and has substantial variability that may influence probability response.Tumor deposition shared mechanism therapeutics such single companion diagnostic agent have utility in predicting response to multiple nanomedicines.Methods: We describe the development, characterization preclinical proof-of-concept positron emission tomography (PET) agent, MM-DX-929, drug-free untargeted 100 nm PEGylated...
A series of novel pyrazolo[1,5-a]pyrimidines, closely related to N,N-diethyl-2-(2-(4-(2-fluoroethoxy)phenyl)-5,7-dimethylpyrazolo[1,5-a]pyrimidin-3-yl)acetamide (2, DPA-714), were synthesized and biologically in vitro evaluated for their potential bind the translocator protein 18 kDa (TSPO), a today recognized as an early biomarker neuroinflammatory processes. This is composed fluoroalkyl- fluoroalkynyl- analogues, prepared from common iodinated intermediate via Sonogashira coupling...
Spatially Targeted Mass Spectrometry (MS) analysis using survey scans with an imaging modality often requires consecutive tissue slices, because of the damage during scan or due to incompatible sample preparation requirements between and MS. We report two spatially targeted MS workflows based on polarized light guidance that use same for analysis. The first workflow is applicable thin-slice analysis, uses transmission-polarimetry-guided Desorption ElectroSpray Ionization (DESI-MS),...
Nanoscale perfluorocarbon (PFC) droplets have enormous potential as clinical theranostic agents. They are biocompatible and currently used
The purpose of the study was to validate [18F]DPA-714, a translocator protein (TSPO) 18 kDa radioligand, as probe non-invasively quantify inflammatory state in bowel disease (IBD) animal models.Quantitative positron emission tomography (PET) imaging intestinal inflammation conducted with 2-deoxy-2-[18F]fluoro-D-glucose ([18F]FDG) glucose metabolism surrogate marker and [18F]DPA-714 ligand TSPO, on two IBD models. first model induced using dextran sodium sulfate (DSS), creating global colon....
Integration between a hand-held mass spectrometry desorption probe based on picosecond infrared laser technology (PIRL-MS) and an optical surgical tracking system demonstrates <italic>in situ</italic> tissue pathology from point-sampled data.
We describe here a simple and versatile route for the preparation of tridentate ligand that not only provides stable interactions with colloidal semiconductor nanocrystals (quantum dots, QDs) but also makes them water soluble. The designed incorporates biocompatible solubilizing polyethylene glycol (PEG) chain connected to thiol motif improved affinity towards QDs surface. Stability experiments revealed better resistance various drastic conditions compared classical dihydrolipoic-based...
Rheumatoid arthritis (RA) is a chronic disease, affecting 0.5 to 1% of adults in industrialized countries, which systemic inflammation and synovitis drive joint destruction. [18F]DPA-714 specific tracer the 18 kDa translocator protein (TSPO), overexpressed on activated macrophages, proposed as biomarker neuroinflammation. Today, diagnosis patients with early inflammatory limited by poor sensitivity specificity. The present study aims investigate potential monitor vivo processes at...
Triple negative breast cancer (TNBC) represents a significant therapeutic challenge due to its highly aggressive nature and lack of effective treatment options. Liposomal irinotecan (nal-IRI, ONIVYDE) was approved in 2015 (by the Food Drug Administration, European Medicines Agency, Therapeutic Goods Administration) is topoisomerase inhibitor indicated, combination with fluorouracil leucovorin, for patients metastatic adenocarcinoma pancreas after disease progression following...
Abstract While photodynamic therapy (PDT) can induce acute inflammation in the irradiated tumor site, a sustained systemic, adaptive immune response is desirable, as it may control growth of nonirradiated distant disease. Previously, we developed porphyrin lipoprotein (PLP), ∼20 nm nanoparticle photosensitizer, and observed that not only efficiently eradicated primary VX2 buccal carcinomas rabbits, but also induced regression metastases draining lymph node. We hypothesized PLP-mediated PDT...
Clinical studies implying the sunitinib multi-kinase inhibitor have led to disappointing results for breast cancer care but mostly focused on HER2-negative subtypes. Preclinical researches involving this drug concern Triple Negative Breast Cancer (TNBC) murine models. Here, we explored therapeutic efficacy of a PyMT-derived transplanted model classified as luminal B (HER2-positive) and monitored response treatment using both in vivo ex approaches.Tumour-induced animals were treated 9 (n = 7)...
Abstract DPA‐C5yne, the lead compound of a novel series DPA‐714 derivatives in which fluoroethoxy chain linked to phenylpyrazolopyrimidine scaffold has been replaced by fluoroalkyn‐1‐yl moiety, is high affinity (K i : 0.35 nM) and selective ligand targeting translocator protein 18 kDa. In present work, DPA‐C5yne was labelled with no‐carrier‐added [ F]fluoride based on one‐step tosyloxy‐for‐fluorine nucleophilic substitution reaction, purified cartridge HPLC, formulated as an i.v. injectable...
Purpose Diagnosis and resection of indeterminate pulmonary nodules (IPNs) is a growing challenge with increased utilization chest computed tomography. Photoacoustic (PA) -guided surgical local injection indocyanine green (ICG) may have utility for IPNs that are suspicious lung cancer. This preclinical study explores the potential PA imaging (PAI) to detect ICG-labeled tumors. Materials methods ICG uptake by H460 cancer cells was evaluated in vitro. A phantom performed analyze signal...
Abstract Objective. Laser interstitial thermal therapy (LITT) is an evolving hyperthermia-based technology that may offer a minimally invasive alternative to inoperable lung cancer. LITT of perivascular targets challenged by higher risk disease recurrence due vascular heat sinks, as well damage these structures. The objective this work examine the impact multiple vessel parameters on efficacy treatment and integrity wall in LITT. Approach. A finite element model used role proximity, flow...
Conventional flexible bronchoscopy has not achieved the high diagnostic yield for intrapulmonary lesions as seen with image-guided transthoracic biopsy. A thin convex probe endobronchial ultrasound bronchoscope (TCP-EBUS) a 5.9-mm tip was designed to improve peripheral access over conventional EBUS bronchoscopes facilitate real-time sampling of under guidance.TCP-EBUS inserted into distal airways ex-vivo human lungs assess bronchial accessibility relative clinically available bronchoscopes....
ObjectiveSevere pulmonary embolism is often managed with thrombolysis. We sought to determine whether endobronchial ultrasound (EBUS)-guided transbronchial thrombolysis remained effective at lower alteplase doses, the goal of minimizing potential bleeding risk.MethodsYorkshire pigs were anesthetized and ventilated. Preformed autologous blood clots administered into bilateral arteries via EBUS-guided injection. After documenting baseline clot sizes, was injected using a 25-gauge needle...
Purpose To evaluate CF800, a novel lipid-based liposomal nanoparticle that co-encapsulates indocyanine green (ICG) and iohexol, for CT imaging of pulmonary vasculature in minimally-invasive thoracic surgery planning. Methods CF800 was intravenously administered to 7 healthy rabbits. In vivo performed 15 min post-injection, with subset animals imaged at 24h, 48h, 72h post injection. Signal-to-background ratios (SBR) were calculated the inferior vena cava compared across time-points. A similar...
Endobronchial ultrasound-guided transbronchial needle injection (EBUS-TBNI) may effectively treat acute pulmonary embolisms (PEs). Here, we assessed the effectiveness of clot dissolution and safety tissue plasminogen activator (t-PA) using EBUS-TBNI in a 1-week survival study porcine PE model.Six pigs with bilateral PEs were used: 3 for t-PA (TBNI group) systemic administration (systemic group). Once created, each 25 mg (a total 50 t-PA) 100 was performed on day 1. Hemodynamic parameters,...
Photodynamic therapy (PDT) and photothermal (PTT) show promise as cancer treatments, but challenges in generating large ablative volumes for deep-seated tumours persist. Using simulations, this study investigates combined PDT PTT to increase treatment volumes, including the impact of a temperature-dependent dose on volume radius. A finite-element model, using open-source SfePy package, was developed simulate interstitial photodynamic treatments. Results compared an additive model with...