A5019726673- Reproductive System and Pregnancy
- MicroRNA in disease regulation
- Effects and risks of endocrine disrupting chemicals
- Pregnancy and preeclampsia studies
- Cleft Lip and Palate Research
- Endometriosis Research and Treatment
- Tissue Engineering and Regenerative Medicine
- Mesenchymal stem cell research
- Folate and B Vitamins Research
- Bone and Dental Protein Studies
- Circular RNAs in diseases
- Reproductive Biology and Fertility
- Pluripotent Stem Cells Research
- Gynecological conditions and treatments
- Epigenetics and DNA Methylation
- Infant Nutrition and Health
- Estrogen and related hormone effects
- Reproductive Physiology in Livestock
- Pregnancy and Medication Impact
- Carcinogens and Genotoxicity Assessment
- Craniofacial Disorders and Treatments
- Congenital heart defects research
- Thyroid Disorders and Treatments
- Arsenic contamination and mitigation
- Urological Disorders and Treatments
National Health and Family Planning Commission
2016-2025
Peking Union Medical College Hospital
2014-2025
Chinese Academy of Medical Sciences & Peking Union Medical College
2016-2025
Tianjin Municipal Research Institute for Family Planning
2015-2022
Genetic Resources Center
2020-2021
Hangzhou Dianzi University
2016-2019
Sun Yat-sen University
2016
China Agricultural University
2015
Chinese Academy of Sciences
2005-2013
Institute of Zoology
2005-2013
Repair deficiency after endometrial injury is an important reason for intra-uterine adhesions, amenorrhea, and infertility in females. Bone marrow-derived mesenchymal stem cell (BMSC) transplantation effective repairing the damaged endometrium. However, possibility of using umbilical cord-derived MSCs (UC-MSCs) to treat rarely reported. Ethanol (95%) was injected into rat uterus establish a model injury. UC-MSCs were through tail vein, either as single, twice, or thrice administration....
The environmental obesogen hypothesis proposes that exposure to endocrine disruptors during developmental 'window' contributes adipogenesis and the development of obesity. MEHP [mono-(2-ethylhexyl) phthalate], a metabolite widespread plasticizer DEHP [di-(2-ethylhexyl) has been found in exposed organisms identified as selective PPARγ (peroxisome-proliferator-activated receptor γ) modulator. However, implication on adipose tissue poorly investigated. In present study, we show dose-dependent...
Although there are plenty of evidence that single-nucleotide polymorphisms (SNPs) fall within coding sequences genes involved in recurrent pregnancy loss (RPL), it is still unknown whether the microRNAs (miRNAs) related with RPL. In this study, we established kind association by confirming significant differences genotype distribution rs41275794 (P= 0.0005) and rs12976445 0.001) pri-miR-125a 217 Han Chinese patients RPL compared 431 controls. Based on observation, two-locus haplotypes were...
Abstract The endometrium plays a critical role in embryo implantation and pregnancy, thin uterus is recognized as key factor failure. Umbilical cord mesenchymal stem cells (UC-MSCs) have attracted interest for the repair of intrauterine adhesions. current study investigated rats using UC-MSCs mechanisms involved. Rats were injected with 95% ethanol to establish model endometrium. randomly divided into normal, sham, model, groups. Endometrial morphological alterations observed by...
Background/Aim: The environmental obesogen hypothesis proposes that exposure to endocrine disruptors during developmental "window" contributes adipogenesis and the development of obesity. Implication disruptor such as 4Nonylphenol (4-NP) on adipose tissue has been poorly investigated. Methods: 3T3-L1 preadipocytes were incubated with different doses 4-NP. Six-week-old C57BL/6J male mice received an intraperitoneal injection vehicle, troglitazone or 4-NP (0.5 mg/kg). Gene expression...
Gestational diabetes mellitus (GDM) is a disease that changes the function of microvascular placenta. MicroRNA (miRNA) expression in placenta may contribute to pathogenesis GDM. Here, we evaluate role and miR-29b development This study discovered was lower placentas derived from patients with GDM than control placentas. MiR-29b over-expression inhibited cell growth migration, knockdown promoted migration. Then predicted confirmed HIF3A direct target two specific binding sites at recognition...
Abstract The potential of arsenic to induce neural tube defects (NTDs) remains a topic controversy. In our previous study, oxidative stress and altered DNA methylation were observed in arsenic‐exposed animal models. However, the correlation between these conditions was not fully understood. Therefore, present aim determine whether exposure results reactive oxygen species levels that affect may contribute NTDs chick embryos. We demonstrated arsenic‐induced associated with stress. Increased...
Embryo implantation is a complex initial step in establishment of successful pregnancy. Many mRNAs have been shown to be differentially expressed the rat uterus during embryo implantation. However, expression profiles microRNAs (miRNAs), key post-transcriptional regulator gene expression, between pre-receptive and receptive phases are still unknown. Here, an miRNA microarray was used examine differential miRNAs phases. Twenty-eight were up-regulated 29 down-regulated at least twofold phase...
Although the relationship between polymorphisms in microRNAs (miRNAs) and recurrent pregnancy loss (RPL) has been studied, there is very little data available literature. In present study, we scanned 55 potentially functional miRNA coding region Chinese women with unexplained RPL (URPL; no. 2011-10). The rs6505162 C>A MIR423 was found to be significantly associated occurrence of human URPL. rare A allele contributed an increase expression mature MIR423. C substitution polymorphism pre-MIR423...
Currently, there is no effective treatment for premature ovarian failure (POF), and stem cell therapy considered the most promising treatment. Human umbilical cord blood mesenchymal cells (hUC-MSCs) have shown good regenerative ability in various diseases, including POF; however, their underlying mechanism dosage POF remain unclear. This study aimed to compare effect of single multiple injections hUC-MSCs on function repair chemotherapy-induced POF.Female mice were intraperitoneally injected...
ABSTRACT Background Our previous study has identified an association of a single nucleotide polymorphism (SNP) in the miR‐423 gene with recurrent spontaneous abortion (RSA). The presence additional RSA‐linked SNPs remains unclear. Methods We evaluated polymorphisms coding region Han Chinese women unexplained RSA (URSA). Results Significant differences were observed genotype and allele distribution rs8067576 between patients control subjects. A robust was found elevated incidence A/T...
The implantation process is complex, requiring reciprocal interactions between implantation-competent blastocysts and the receptive uterus. There were reports to show that some microRNAs (miRNAs) may play a key role during embryo in mouse. However, miR-320 expression profiles rat uterus peri-implantation are unknown. In present study, we found level of was lower on day 5 gestation (g.d. 5) rats than g.d.3 g.d.4 restored gradually from g.d.6. MiR-320 specifically localized glandular luminal...
Abstract Vitamin A (VA) is required for normal fetal development and successful pregnancy. Excessive VA intake during pregnancy may lead to adverse maternal effects. Cytochrome P450 26A1 (cyp26a1), a retinoic acid (RA)‐metabolizing enzyme, involved in metabolism. It has been shown that cyp26a1 expressed female reproductive tract, especially uterus. In order investigate the role of pregnancy, we constructed recombinant plasmid DNA vaccine encoding protein immunized mice with plasmid. Compared...
Two experiments were conducted, under typical commercial swine production conditions, to determine effects of dietary arginine supplementation during early gestation on the performance gilts and sows. In Exp. 1, between d 1 30 gestation, 62 Landrace 113 sows consumed a corn- soybean meal-based diet supplemented with 1.3% -arginine HCl or 2.2% -alanine. Total numbers piglets born ( < 0.05) alive 0.01) per litter birth weights increased in group compared control. 2, 155 multiparous received 14...
In a previous study, via microRNA microarray analysis we found that miR‐98 is differentially expressed in rat uteri during the peri‐implantation period (unpublished data). However, role of embryo implantation remains elusive. Here, level lower on day 5 and 6 gestation (g.d. 5–6) than g.d. 3–4 7–8 rat. MiR‐98 expression significantly decreased by delayed implantation. Down‐regulation promotes ESC proliferation inhibits apoptosis. Up‐regulation displays opposite effects. Further investigation...
Purpose: This study aimed to investigate the role of miR-21 expression in reduction placental function GDM patients. Materials and Methods: qRT-PCR was used detect differential serum gestational diabetes mellitus (GDM) normal pregnant women, verify functional target gene PPAR-α by double fluorescence experiments. Cellular experiments were performed effect on cell function. Results: is down-regulated placenta patients compared women. In case insulin resistance, miR-21-5p knockdown promoted...
Mammalian embryonic implantation requires reciprocal interactions between implantation-competent blastocysts and a receptive uterus. Some microRNAs might play key role during embryo in the mouse, but let-7a expression profiles rat uterus peri-implantation are unknown. In study, of early pregnancy, pseudopregnancy, artificial decidualization activation delayed was detected by Northern blotting situ hybridization. The effect steroid hormones on also Here, we found that level higher gestation...
Dibutyl phthalate (DBP), a widely used phthalate, is known to cause many serious diseases, especially in the reproductive system. However, little about effects of its metabolite, mono-n-butyl (MBP), on preimplantation embryo development. In present study, we found that treatment embryos with 10–3 M MBP impaired developmental competency, whereas exposure 10–4 delayed progression blastocyst stage. Furthermore, reactive oxygen species (ROS) levels were significantly increased following MBP....
Neural tube defects (NTDs) are one of the most common types birth defects, affecting approximately 1 every 1000 pregnancies in United States and an estimated 300 000 newborns worldwide each year. The metabolic signature pregnant women with NTDs offspring has not previously been characterized. In this paper, we report a profiling study that characterized serum metabolome 101 affected comparison 143 normal pregnancy outcomes Lvliang prefecture, area highest prevalence China. A metabonomic was...
Dual-oxidase maturation factor 2 (DUOXA2) is a component of the thyroid hydrogen peroxidase (H2O2) generator, which crucial for hormone synthesis. Genetic defects in DUOXA2 lead to an impairment H2O2-generating system, turn causing congenital hypothyroidism (CH) with goiter.Our study aimed identify mutations associated Chinese goiter patients and examine molecular mechanism underlying genotype-phenotype relationship. All exons flanking sequences 75 unrelated CH were amplified by PCR directly...