A5079960595- Microbial Metabolic Engineering and Bioproduction
- Helicobacter pylori-related gastroenterology studies
- Shoulder Injury and Treatment
- Fungal and yeast genetics research
- Liver Disease Diagnosis and Treatment
- Vestibular and auditory disorders
- Intracranial Aneurysms: Treatment and Complications
- Musculoskeletal synovial abnormalities and treatments
- Foot and Ankle Surgery
- Bone Metabolism and Diseases
- Synthesis and biological activity
- Venous Thromboembolism Diagnosis and Management
- Biofuel production and bioconversion
- Tendon Structure and Treatment
- Gout, Hyperuricemia, Uric Acid
- Medical Research and Treatments
- Glaucoma and retinal disorders
- Mollusks and Parasites Studies
- Hearing, Cochlea, Tinnitus, Genetics
- Cerebrovascular and Carotid Artery Diseases
- Cell death mechanisms and regulation
- Viral gastroenteritis research and epidemiology
- Antifungal resistance and susceptibility
- Silymarin and Mushroom Poisoning
- Paraoxonase enzyme and polymorphisms
Zhejiang Chinese Medical University
2024-2025
Hengyang Normal University
2025
Binzhou University
2024
Yantaishan Hospital
2008-2024
Binzhou Medical University
2024
Henan Cancer Hospital
2024
Zhengzhou University
2002-2024
West China Medical Center of Sichuan University
2024
Hospital of Hebei Province
2024
Chinese PLA General Hospital
2013-2022
Abstract Acetyl-CoA is a fundamental metabolite for all life on Earth, and also key starting point the biosynthesis of variety industrial chemicals natural products. Here we design construct Synthetic (SACA) pathway by repurposing glycolaldehyde synthase acetyl-phosphate synthase. First, engineer to improve catalytic activity more than 70-fold, condense two molecules formaldehyde into one glycolaldehyde. Second, repurpose phosphoketolase convert acetyl-phosphate. We demonstrated feasibility...
Objective: Persistent postural-perceptual dizziness (PPPD) is a common cause of chronic dizziness, but only few studies have reported its clinical characteristics, and no related research has been performed in China. Therefore, the purpose this study was to analyze characteristics PPPD for first time Design: Data collected from all patients during standard practice, further evaluate comparing with control group. Study sample: A total 43 diagnosed were selected as group analysis. Results:...
This study investigated the fructo-oligosaccharides (FOS) on growth performance and meat quality in broilers. Total 160 Xianghuang broilers aged 2 months were randomly assigned into groups, CON (control), FOS (supplemented 0.5% diet). After 38 days, breast, thigh muscle liver samples collected for further analysis. Results showed that no significant effect of such as average daily gain (ADG), feed intake (ADFI) or feed-to-gain ratio (F:G) observed (P > 0.05). Broilers group had a yellower...
This study seeks to confirm the therapeutic effectiveness of TRFD in inhibiting adipogenesis and promoting osteogenesis primary osteoporosis through MAPK/HIF-1α signaling pathway. C57BL/6J mice underwent ovariectomy (OVX) induce osteoporosis. Mice were administered (Low high doses)estradiol for a duration 12 weeks. Bone microarchitecture evaluated using Micro-CT, while serum biomarkers protein expressions analyzed enzyme-linked immunosorbent assay, Western blotting, immunohistochemistry....
Abstract MDF1 is a young de novo -originated gene from non-coding sequence in baker's yeast, S. cerevisiae , which can suppress mating and promote vegetative growth. Our previous experiments successfully demonstrated how Mdf1p binds to the key pathway determinant MATα2 mating. However, promotes growth fulfills crosstalk between yeast pathways are still open questions. Thus, adaptive significance of this new remains speculative. Here, we show that shortens lag phase by physically interacting...
Abstract Protein rational design has become more and popular for protein engineering with the advantage of biological big-data. In this study, we described a method that is able to identify desired mutants by analyzing coevolution sequence. We employed approach evolve an archaeal isopentenyl phosphate kinase can convert dimethylallyl alcohol (DMA) into precursor isoprenoids. By designing 9 point mutations, improved catalytic activities IPK about 8-fold in vitro . After introducing optimal...
Background: Asparagine Synthetase Deficiency (ASNSD; OMIM #615574) is a newly described rare autosomal recessive neurometabolic disorder, characterised by congenital microcephaly, severe psychomotor delay, encephalopathy and progressive cerebral atrophy. To date, seven families missense mutations in the ASNSD disease causing gene, ASNS, have been published. Methods: We report two further affected infant sisters from consanguineous Indian family, who addition to previously features had...
Type 2C protein phosphatase (PP2C) is a monomeric enzyme and requires Mg(2+) or Mn(2+) for its activity. Up to now, seven PP2C-like genes have been identified in the genome of Saccharomyces cerevisiae. However, encoded by sixth gene, YCR079w, has not demonstrated PP2C In this study, we show that YCR079w confers rapamycin-resistant function yeast cells, also demonstrate YCR079w-encoded exhibits characteristics typical PP2C. Therefore, encodes PP2C, PTC6, budding yeast.
In order to investigate the function of toll-like receptor 4/nuclear factor-kappa B (TLR4/NF-κB) signal pathways in pathogenesis diarrhea-predominant irritable bowel syndrome (IBS-D), IBS-D animal models were established wistar rats challenged with acute and chronic stresses (29 days). Wistar without stress-challenged used as controls. randomly divided into two groups: one was treated normal saline, another group TLR4/NF-κB inhibitor, pyrrolidine dithiocarbamate (PDTC) (50mg/kg/week) for...
The anatomical location for lateral ligament reconstruction remains a significant challenge. With the development of arthroscopic technology, arthroscopic-assisted can significantly facilitate correct localization points. However, there is limited evidence on clinical efficacy compared with percutaneous reconstruction. This study included 72 patients who underwent chronic ankle instability from 2018 to 2022. follow-up duration in percutaneous(n = 35) and arthroscopic(n 37) groups was 6–30...
Summary Acute kidney injury ( AKI ) is a common complication in patients with decompensated cirrhosis and also an important cause for poor outcome. This study aimed at investigating the clinical characteristics long‐term prognosis of hepatitis B virus HBV )‐related acute‐on‐chronic liver failure ACLF ). A total 1167 ‐related from January 2010 to 2015 were enrolled divided into two groups, group (n=308) non‐ (n=859). All followed up investigate characteristics, overall survival OS risk...
Objective To explore specific flora in mouse models of non-alcoholic steatohepatitis (NASH) to improve NASH diagnostic protocols. Methods Sixty mice were divided into normal diet (ND, 20 mice) and high-fat/high-sugar (HFSD) groups (40 mice). After 8 weeks feeding, 10 the ND group HFSD sacrificed create short-term fatty liver (NAFL) groups, respectively. 16 remaining long-term We then examined fecal flora, serum biochemical indices, lipopolysaccharide tumor necrosis factor-α levels analyzed...
Type 2C protein phosphatases (PP2C) represent a diversified phosphatase family and play various roles in cells. We previously identified characterized novel PP2C encoded by the CaPTC7 gene human fungal pathogen Candida albicans . The CaPtc7p has 365 amino acids with core domain at C terminus an additional 116-residue N-terminal sequence containing mitochondrion-targeting sequence. Here, we show that is indeed localized mitochondrion, only eukaryotic been directly shown to reside suggesting...
Medication overuse headache (MOH) is the third most prevalent type after migraine and tension-type headache. A large number of studies on long-term prognosis have shown that MOH has a high relapse rate treatment. Although relapse-related risk factors been reported, no related research performed in China. Therefore, purpose this study was to analyze evaluate for Eighty-six out-patients Shandong Provincial Hospital who were initially diagnosed with MOH, had successful withdrawal treatment...