A5100367460- Viral gastroenteritis research and epidemiology
- Animal Virus Infections Studies
- Virus-based gene therapy research
- Animal Disease Management and Epidemiology
- SARS-CoV-2 and COVID-19 Research
- Seedling growth and survival studies
- Agronomic Practices and Intercropping Systems
- RNA modifications and cancer
- Viral Infections and Vectors
- ATP Synthase and ATPases Research
- Herpesvirus Infections and Treatments
- Viral Infections and Immunology Research
- Virology and Viral Diseases
- Mitochondrial Function and Pathology
- RNA and protein synthesis mechanisms
- Plant nutrient uptake and metabolism
- interferon and immune responses
Center of Hubei Cooperative Innovation for Emissions Trading System
2018-2023
Nanjing Forestry University
2023
Huazhong Agricultural University
2017-2023
Shandong Agricultural University
2023
Coronaviruses cause widespread respiratory, gastrointestinal, and central nervous system diseases in humans other animals, threatening human health causing economic loss. Coronavirus nsp9, a member of the replication complex, is an important RNA binding subunit RNA-synthesizing machinery all coronaviruses. However, mechanisms dimerization nucleic acid nsp9 remain elusive. In this study we determined crystal structures PDCoV PEDV. We first found that N-finger from plays critical role...
African swine fever (ASF), caused by virus (ASFV), is a highly contagious and acute hemorrhagic viral disease with high mortality, approaching 100% in domestic pigs. However, the relationship between pathogenicity immune evasion of ASFV not fully understood, which limits development safe effective ASF vaccines, specifically, live attenuated vaccines.
ABSTRACT Porcine epidemic diarrhea virus (PEDV), an enteropathogenic Alphacoronavirus , has caused enormous economic losses in the pork industry. Nonstructural protein 1 (nsp1) is a characteristic feature of alpha- and betacoronaviruses, which exhibits both functional conservation mechanistic diversity inhibiting host gene expression antiviral responses. However, detailed structure molecular mechanisms underlying nsp1 inhibition remain unclear. Here, we report first full-length crystal from...
Transmissible gastroenteritis virus (TGEV) is the etiologic agent of transmissible in pigs, and N-terminal domain TGEV spike protein generally recognized as both virulence determinant enteric tropism determinant. Here, we assembled a full-length infectious cDNA clone bacterial artificial chromosome. Using novel approach, clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated 9 (Cas9) systems efficiently rapidly rescued another recombinant with 224-amino-acid...
Feline infectious peritonitis virus (FIPV) is the etiologic agent of feline (FIP) and causes fatal disease in cats almost all ages. Currently, there are no clinically approved drugs or effective vaccines for FIP. Furthermore, pathogenesis FIP still not fully understood. There an urgent need infection model induced by FIPV. Here, we constructed a field type I FIPV full-length cDNA clone, pBAC-QS, corresponding to isolated QS. By replacing QS spike gene with commercially available II 79-1146...
African swine fever, caused by the fever virus (ASFV), is among most significant diseases. There are currently no effective treatments against ASFV. ASFV contains a gene encoding dUTPase (E165R), which required for viral replication in macrophages, making it an attractive target inhibitor development. However, full structural details of and those comparable enzyme not available, limiting further insights. Herein, we determine crystal structures both their ligand-free ligand-bound forms. We...
Understanding the role of endoribonuclease non-structural protein 15 (nsp15) (EndoU) in coronavirus (CoV) infection and pathogenesis is essential for vaccine target discovery. Whether EndoU activity CoV nsp15, as a virulence-related protein, has diverse effect on viral virulence needs to be further explored. Here, we found that transmissible gastroenteritis virus (TGEV) feline infectious peritonitis (FIPV) nsp15 proteins antagonize SeV-induced interferon-β (IFN-β) production human embryonic...