A5005600338- Pluripotent Stem Cells Research
- Hearing, Cochlea, Tinnitus, Genetics
- Tissue Engineering and Regenerative Medicine
- Tracheal and airway disorders
- Mesenchymal stem cell research
- Marine animal studies overview
- Cancer-related molecular mechanisms research
- Neonatal Respiratory Health Research
- CRISPR and Genetic Engineering
- Luminescence Properties of Advanced Materials
- Developmental Biology and Gene Regulation
- Congenital heart defects research
- Neurogenetic and Muscular Disorders Research
- Glycosylation and Glycoproteins Research
- Cell Adhesion Molecules Research
- Alkaline Phosphatase Research Studies
- Barrier Structure and Function Studies
- Quantum Dots Synthesis And Properties
- Monoclonal and Polyclonal Antibodies Research
- Vestibular and auditory disorders
- Head and Neck Surgical Oncology
- Congenital Ear and Nasal Anomalies
- Semiconductor Quantum Structures and Devices
- Voice and Speech Disorders
- Ear Surgery and Otitis Media
Kyoto University
2013-2025
Shiga Medical Center
2021
Kyoto University Hospital
2016-2019
National Institute of Advanced Industrial Science and Technology
2010-2015
Ehime University
1983-2010
Osaka University
1983-2008
Kindai University
2000
The expression of four transcription factors (<i>OCT3/4</i>, <i>SOX2</i>, <i>KLF4</i>, and <i>MYC</i>) can reprogram mouse as well human somatic cells to induced pluripotent stem (iPS) cells. We generated iPS from mesenchymal stromal (MSCs) derived third molars (wisdom teeth) by retroviral transduction <i>OCT3/4</i>, <i>KLF4</i> without <i>MYC</i>, which is considered oncogene. Interestingly, some the clonally expanded MSCs could be used for cell generation with 30–100-fold higher efficiency...
Non-neuronal acetylcholine (ACh) is predicted to function as a local cell signaling molecule. However, the physiological significance of synthesis non-neuronal ACh in intestine remains unclear. Here, experiments using crypt-villus organoids that lack nerve and immune cells culture led us suggest endogenous synthesized intestinal epithelium evoke growth differentiation through activation muscarinic receptors (mAChRs). The extracts cultured showed noticeable capacity for was sensitive potent...
Human adipose-derived stem cells (hASCs) are ubiquitous, plentiful, and easily/safely obtainable derived from adipose tissue, regardless of the age sex donor. However, hASCs have limited proliferative differentiation capabilities. In this study, we examined whether induced pluripotent (iPSCs) could be generated hASCs. We transduced with three human transcription factors (OCT3/4, SOX2, KLF4), found that they formed embryonic cell (ESC)–like colonies. Importantly, did not transduce c-MYC,...
Early B-cell factor 1 (EBF1) is a basic helix-loop-helix transcription essential for the differentiation of various tissues. Our single-cell RNA sequencing data suggest that Ebf1 expressed in sensory epithelium mouse inner ear. Here, we found murine gene and its protein are prosensory domain ear, medial region cochlear duct floor, otic mesenchyme, cochleo-vestibular ganglion. deletion mice results incomplete formation spiral limbus scala tympani, increased number cells organ Corti Kölliker's...
Information on the maintenance of tissue homeostasis is important for development effective therapeutic methods, however, reports cellular composition and stem cells larynx were scarce. Therefore, we analyzed mouse laryngeal mucosa using scRNA-seq, spatial transcriptomics by photo-isolation chemistry, performed generation organoid used as an in vitro model from mucosa. As a result, found SOX9-positive basal cell subpopulation Lgr5-positive fibroblast vocal fold, obtained three types...
The apical junctional complex is composed of various cell adhesion molecules and cytoplasmic plaque proteins. Using a monoclonal antibody that recognizes chicken 155-kDa antigen (p155) localizing at the complex, we have cloned cDNA its mouse homologue. full-length p155 encoded 148-kDa polypeptide containing coiled-coil domain with sequence similarity to cingulin, tight junction (TJ)-associated protein. We designated this protein JACOP (junction-associated protein). Immunofluorescence...
Patients with severe hypophosphatasia (HPP) develop osteogenic impairment extremely low alkaline phosphatase (ALP) activity, resulting in a fatal course during infancy. Mesenchymal stem cells (MSCs) differentiate into various mesenchymal lineages, including bone and cartilage. The efficacy of allogeneic hematopoietic cell transplantation for congenital skeletal storage disorders is limited, therefore we focused on MSCs the treatment HPP. To determine effect osteogenesis, performed multiple...
The present study examined the efficacy of a neural induction method for human induced pluripotent stem (iPS) cells to eliminate undifferentiated and determine feasibility transplanting neurally into guinea-pig cochleae replacement spiral ganglion neurons (SGNs). A stepwise differentiation iPS was used. First, established on Matrigel-coated plates; characteristics cell populations at each step were assessed. Second, differentiated three-dimensional (3D) collagen matrix, using same protocol...
The laryngotracheal cartilage is a cardinal framework for the maintenance of airway breathing, which occasionally requires reconstruction. Because hyaline has poor intrinsic regenerative ability, various approaches have been attempted to regenerate cartilage. use autologous mesenchymal stem cells (MSCs) regeneration widely investigated. However, long-term culture may limit proliferative capacity. Human-induced pluripotent cell-derived MSCs (iMSCs) can circumvent this problem due their...
Spontaneous mutations have been isolated in Escherichia coli that result the constitutive expression of an emrKY promoter. These were found to be single-nucleotide substitutions within linker region sensor protein EvgS, which is part a two-component regulatory system along with EvgA. In mutants (evgS1 and evgS4), became MIC against sodium deoxycholate was 20 mg/ml, eight-fold higher than wild type. Furthermore, start site transcription from promoter identified; EvgA shown bind at -52 -84 by...
We previously reported that expression of CD43/leukosialin induces cell rounding and microvillus formation via inhibition adhesion. Here, we found CD34, a surface sialomucin marker for hematopoietic progenitor cells, also inhibited adhesion induced formation. Forced CD34-induced rounding, formation, phosphorylation ezrin/radixin/moesin (ERM) proteins in HEK293T while inhibiting integrin-mediated re-attachment. Furthermore, CD34+ blood cells KG-1 which express endogenous CD34 on their...
Tracheal resection is often performed for malignant tumours, congenital anomalies, inflammatory lesions, and traumatic injuries. There no consensus on the best approach restoration of tracheal functionality in patients with defects. Artificial grafts made polypropylene collagen sponge have been clinically used by our group. However, 2 months are required to achieve adequate epithelialization humans. This study aimed investigate feasibility transplantation therapy using an artificial trachea...
Fast-dissociating, specific antibodies are single-molecule imaging probes that transiently interact with their targets and used in biological applications including image reconstruction by integrating exchangeable localization (IRIS), a multiplexable super-resolution microscopy technique. Here, we introduce semi-automated screen based on total internal reflection fluorescence (TIRF) of antibody-antigen binding, which allows for identification fast-dissociating monoclonal directly from...
In contrast to mammals, the avian cochlea, specifically basilar papilla, can regenerate sensory hair cells, which involves fate conversion of supporting cells cells. To determine mechanisms for converting we used single-cell RNA sequencing during cell regeneration in explant cultures chick papillae. We identified dynamic changes gene expression and pseudotime trajectory analysis demonstrated stepwise from Initially, identity was erased transition precursor state occurred. A subsequent gain...
Etude de l'electroluminescence couches minces ZnS activees par TbF 3 et Mn, en fonction la concentration impuretes l'epaisseur couche
To regenerate defected recurrent laryngeal nerves (RLNs), various methods have been developed. However, no consistently effective treatments are currently available because of their insufficient functional recovery. RADA16-I, a self-assembling peptide used clinically as hemostat, reportedly supports neurite outgrowth and synapse formation in vitro. The purpose this study was to investigate the effect RADA16-I hydrogels on transected RLNs rats.Animal experiments with controls.Fifteen adult...
ABSTRACT Enteropathogenic Escherichia coli , Shigella flexneri and Listeria monocytogenes induce localized actin polymerization at the cytoplasmic face of plasma membrane or within host cytoplasm, creating unique actin-rich structures termed pedestals tails. The process is known to be mediated by actin-related protein 2 3 (Arp2/3) complex, which in these cases acts downstream neural Wiskott-Aldrich syndrome (N-WASP) a listerial functional homolog WASP family proteins. Here, we show that...
Bone marrow-derived mesenchymal stem cells (BMSCs) and adipose tissue-derived (AMSCs) have been used clinically for tissue regeneration; however, their proliferation/differentiation potentials are limited. Recently, induced pluripotent (iPSCs), known to nearly unlimited potential proliferate differentiate into of all three germ layers, gained wide interest in regenerative medicine. Here, we generated iPSCs from frozen-stocked AMSCs BMSCs examined biological characteristics by comparative...
CD43/sialophorin/leukosialin, a common leukocyte antigen, is known as an inhibitor for cell adhesion. The ectodomain of CD43 considered molecular barrier adhesion, while the cytoplasmic domain has binding site Ezrin/Radixin/Moesin (ERM). We found expression induced rounding, inhibition re-attachment, augmentation microvilli, and phosphorylation ERM in HEK293T cells. Mutant studies revealed CD43, but not intracellular domain, essential sufficient all these phenomena. also that forced...
Early postoperative regeneration of the middle ear mucosa is essential for prevention refractory otitis media and recurrent cholesteatoma. As a means intractable management, we focused on human induced pluripotent stem cell (hiPSC)-derived airway epithelial cells (AECs), which have been used in upper mucosal transplantation therapy. In this study, transplanted hiPSC-derived AECs into immunodeficient rats.Following preparation AEC sheets from hiPSCs, bilateral X-linked severe combined rats...
Luminescence is the emission of light, other than thermal incandescence, as a result external stimulation material. A material that shows efficient luminescence called phosphor. Numerous phosphors have been developed are important emissive-type electronic display materials for cathode ray tubes, vacuum fluorescent devices, color plasma panels, electroluminescent and, matter course, lamps. Types distinguished by source energy input. Photoluminescence involves excitation optical radiation,...
Hair cells are important for maintaining our sense of hearing and balance. However, they difficult to regenerate in mammals once lost. Clarification the molecular mechanisms underlying inner ear disorders is also impeded by anatomical limitation experimental access human ear. Therefore, generation hair cells, possibly from induced pluripotent stem (iPS) regenerative therapy studies diseases.We generated mouse iPS using an established stepwise induction protocol. First, were differentiated...