A5087708346- Tracheal and airway disorders
- Voice and Speech Disorders
- Tissue Engineering and Regenerative Medicine
- Dysphagia Assessment and Management
- Recycling and utilization of industrial and municipal waste in materials production
- Neonatal Respiratory Health Research
- Osteoarthritis Treatment and Mechanisms
- Metallurgical Processes and Thermodynamics
- Cancer-related molecular mechanisms research
- Advanced ceramic materials synthesis
- Iron and Steelmaking Processes
- Magnesium Oxide Properties and Applications
- Proteoglycans and glycosaminoglycans research
- Autophagy in Disease and Therapy
- Mercury impact and mitigation studies
- Air Quality and Health Impacts
- Innovations in Concrete and Construction Materials
- Lanthanide and Transition Metal Complexes
- Calibration and Measurement Techniques
- Epigenetics and DNA Methylation
- MRI in cancer diagnosis
- Hippo pathway signaling and YAP/TAZ
- Wound Healing and Treatments
- Material Properties and Applications
- RNA Research and Splicing
Kitasato University
2011-2025
New York University
2020-2025
Saitama University
2025
Teikyo University Hospital
2024
Kyoto University
2016-2021
University of Yamanashi
2018-2021
Osaka University
2004-2020
East Japan Railway (Japan)
2018
Fukushima Medical University
2015-2017
The University of Tokyo
2013-2016
Snail, also called Snai1, is a key regulator of EMT. Snail plays crucial roles in cancer progression, including resistance to anti-tumor drugs and invasion by various cells. Slug, known as Snai2, involved the aggravation certain tumors. In this study, we examined Slug human oral squamous cell carcinoma (OSCC) highly expressed these cells, siRNA effectively represses drug invasive properties. addition, transforming growth factor (TGF)-β upregulates expression promotes OSCC Surprisingly,...
Abstract Many postsynaptic density proteins carrying density‐95/discs large/zone occludens‐1 (PDZ) domain(s) interact with glutamate receptors to control receptor dynamics and synaptic plasticity. Here we examined the expression of PDZ proteins, synapse‐associated protein (SAP) 97, (PSD)‐95, chapsyn‐110, GRIP1 SAP102, in post‐mortem brains schizophrenic patients subjects, evaluated their contribution pathology. Among these SAP97 exhibited most marked change: levels were decreased less than...
A bstract : DNA microarrays with isotope labeling from gene‐specific primers enable sensitive detection of rare mRNAs, including neurotrophin and cytokine mRNAs in the brain. Using high‐quality RNA postmortem brains, gene‐expression profiles covering 1373 genes were assessed dorsoprefrontal cortex schizophrenic patients compared those nonpsychiatric subjects. Statistical analysis microarray data confirmed findings a previous GeneChip study by Hakak et al. (Proc. Natl. Acad. Sci. USA Vol. 98,...
Dynamic range is becoming a key performance parameter for CMOS image sensors (CISs), especially the surveillance and automotive fields. Several well-known multi-exposure methods are widely used to expand dynamic (DR). However, those cause issues such as motion artifacts light emitting diode (LED) flickering with composite images. Therefore, single-exposure method critical quality of high-DR The most obvious way DR by employing in-pixel capacitors. One technologies capacitors lateral overflow...
Abstract ETS homologous factor (EHF) belongs to the epithelium-specific subfamily of E26 transformation-specific (ETS) transcription family. Currently, little is known about EHF’s function in cancer. We previously reported that ETS1 induces expression ZEB family proteins ZEB1/δEF1 and ZEB2/SIP1, which are key regulators epithelial–mesenchymal transition (EMT), by activating ZEB1 promoters. have found EHF gene produces two transcript variants, namely a long form variant includes exon 1...
Abstract Vocal fold (VF) fibrosis is a major cause of intractable voice-related disability and reduced quality life. Excision fibrotic regions suboptimal associated with scar recurrence and/or further iatrogenic damage. Non-surgical interventions are limited, putatively related to limited insight regarding biochemical events underlying fibrosis, downstream, the lack therapeutic targets. YAP/TAZ integrates diverse cell signaling interacts pathways including TGF-β/SMAD pathway. We investigated...
The reactivity of pyridoxal-5'-phosphate (PLP) with cysteine and its derivatives has been increasing interest because the corresponding product, a thiazolidine PLP-cysteine adduct, can be formed via PLP-dependent enzymatic non-enzymatic reactions. Here, we report biochemical X-ray crystallographic snapshots formation in reaction D-cysteine PLP SufS, L-cysteine desulfurase. By comparing L- D-penicillamine-bound SufS showing no crystals proposed mechanism important factors: polar environments...
The pro-fibrotic effects of glucocorticoids may lead to a suboptimal therapeutic response for vocal fold (VF) pathology. Targeting macrophage-fibroblast interactions is an interesting strategy; macrophages alter their phenotype mediate both inflammation and fibrosis. In the current study, we investigated concentration-dependent methylprednisolone on fibrotic response, with emphasis YAP/TAZ-TEAD signaling, inflammatory gene expression in VF fibroblasts physical contact macrophages. We sought...
Macrophage phenotypes are simplistically classified as pro-inflammatory (M1) or anti-inflammatory/pro-fibrotic (M2). Phenotypically different macrophages putatively involved in vocal fold (VF) fibrosis. The current study investigated interactions between and VF fibroblasts. THP-1 monocyte-derived were treated with interferon-gamma (IFN-γ), lipopolysaccharide (LPS)/IFN-γ, interleukin-10 (IL10), transforming growth factor-β1 (TGF-β), interleukin-4 (IL4) for 24 h (M(IFN), M(IFN/LPS), M(IL10),...
The Spectral Profiler (SP) is a visible-near infrared spectrometer on board the Japanese Selenological and Engineering Explorer, which was launched in 2007 observed Moon until June 2009. SP consists of two gratings three linear-array detectors: VIS (0.5-1.0 μm ), NIR 1 (0.9-1.7 μm), 2 (1.7-2.6 μm). In this paper, we characterize radiometric spectral properties using in-flight observational data as well preflight derived laboratory experiments calibrated integrating sphere. We also proposed...
Tracheal resection is often performed for malignant tumours, congenital anomalies, inflammatory lesions, and traumatic injuries. There no consensus on the best approach restoration of tracheal functionality in patients with defects. Artificial grafts made polypropylene collagen sponge have been clinically used by our group. However, 2 months are required to achieve adequate epithelialization humans. This study aimed investigate feasibility transplantation therapy using an artificial trachea...
Tracheal epithelia have barrier and mucociliary clearance functions that prevent invasion of extraneous particles infectious materials. Hence, following tracheal reconstructions, functional morphological regeneration is required to respiratory declines diseases. Although growth factors (GFs) promote the epithelial morphologies, it remains unclear whether grafts containing GFs are beneficial for functions. Thus, we fabricated collagen sponge scaffolds insulin-like GF-1 (IGF-1) basic...
To regenerate defected recurrent laryngeal nerves (RLNs), various methods have been developed. However, no consistently effective treatments are currently available because of their insufficient functional recovery. RADA16-I, a self-assembling peptide used clinically as hemostat, reportedly supports neurite outgrowth and synapse formation in vitro. The purpose this study was to investigate the effect RADA16-I hydrogels on transected RLNs rats.Animal experiments with controls.Fifteen adult...
Diclofenac etalhyaluronate, an active pharmaceutical ingredient in JOYCLU® (JCL), serves as a joint function improvement agent knee and hip osteoarthritis patients. However, frequent cases of anaphylaxis induced by JCL administration have been reported. Recent clinical research suggests the potential utility basophil activation test (BAT) predicting JCL-induced anaphylaxis. Nonetheless, BAT is deemed impractical for routine diagnostic testing due to complex procedures involving whole blood...
It has been shown that pulmonary exposure to diesel exhaust particles (DEP) disrupt immune systems, presenting as exacerbating effects on allergic manifestations (e.g., asthma). To date, the impact of nano-level DEP health not fully elucidated. Our institute (the National Institute for Environmental Studies) established an 'environmental nanoparticle system applied in animals' 2005 and, since then, exposures these types agents have explored. The present study was designed investigate vitro...
Perlecan is a multifunctional component of the extracellular matrix. It shows different effects on distinct cell types, and therefore it thought to show potential for therapies targeting multiple types. However, full range multifunctionality perlecan remains be elucidated. We cultured various which were derived from epithelial/endothelial, connective muscle tissues, in presence either antiserum against or exogenous perlecan, examined migration proliferation. Cell was determined using scratch...
Objective Glucocorticoids (GCs) modulate multiple cellular activities including inflammatory and fibrotic responses. Outcomes of GC treatment for laryngeal disease vary, affording opportunity to optimize treatment. In the current study, three clinically employed GCs were evaluated identify optimal in vitro concentrations at which mediate favorable anti‐inflammatory effects cell types. We hypothesize a therapeutic window will emerge as foundation optimized strategies patients with disease....
Although it has been shown that exposure to diesel exhaust (DE) is linked the induction or exacerbation of respiratory disorders, major components responsible have not fully identified. We examined effects airway nanoparticle-rich DE (NR-DE) without particles on allergic pulmonary inflammation in mice. also investigated cellular responses intratracheal instillation NR-DE (NR-DEP). ICR mice inhaled one four different mixtures (control air, low-concentration DE, high-concentration and...
Abstract Multiciliated epithelial cells in the airway are essential for mucociliary clearance. Their function relies on coordinated, metachronal and directional ciliary beating, appropriate mucus secretion surface hydration. However, current conventional methods observing human movement require ciliated to be detached from tissues. Determining directionality of cilia is difficult. We developed a novel method stain observe their without releasing cells. Human tracheae were obtained patients...
Hypoxia-inducible factor-1alpha (HIF-1alpha) is a transcriptional factor that regulates genes involved in the response to hypoxia. We evaluated effects of HIF-1alpha over expression on tumorigenic potency renal cell carcinoma VMRC cells and bladder cancer EJ vitro vivo.We introduced vectors into cells, generated expressing lines VMRC-HIF1alpha EJ-HIF1alpha, vector only transfected VMRC-neo EJ-neo. then proliferation vivo tumor growth these after subcutaneous injection athymic nude mice.In...