A5065938624- Voice and Speech Disorders
- Tracheal and airway disorders
- Head and Neck Cancer Studies
- Head and Neck Surgical Oncology
- Dysphagia Assessment and Management
- Salivary Gland Tumors Diagnosis and Treatment
- Cleft Lip and Palate Research
- Ear and Head Tumors
- Phonetics and Phonology Research
- Head and Neck Anomalies
- Thyroid and Parathyroid Surgery
- Cancer Diagnosis and Treatment
- Oral and Maxillofacial Pathology
- Tumors and Oncological Cases
- Lung Cancer Treatments and Mutations
- Respiratory and Cough-Related Research
- Hearing, Cochlea, Tinnitus, Genetics
- Wound Healing and Treatments
- Mesenchymal stem cell research
- Cancer-related molecular mechanisms research
- Hearing Loss and Rehabilitation
- Neuroendocrine Tumor Research Advances
- Bone Tumor Diagnosis and Treatments
- Tissue Engineering and Regenerative Medicine
- Neurobiology of Language and Bilingualism
Fujita Health University
2019-2024
Fujita Health University Hospital
2023
Kyoto University
2013-2022
Google (United States)
2009-2021
Kyoto University Hospital
2003-2010
University of Wisconsin–Madison
2004-2008
Kyoto Katsura Hospital
2008
UW Health University Hospital
2007
Madison Group (United States)
2004
Shimane Prefectural Central Hospital
2001
This study aimed to clarify the characteristics of rat vocal fold scarring by examining alteration key components in extracellular matrix: hyaluronic acid, collagen, and fibronectin. Under monitoring with a 1.9-mm-diameter telescope, unilateral stripping was performed, larynges were harvested at 2, 4, 8, 12 weeks after operation. The folds histologically analyzed Alcian blue stain, trichrome immunofluorescence collagen type I, III, scarred showed less acid more types I III than did controls...
A good prognosis can be expected for most, but not all, cases of thyroid papillary cancer. Numerous molecular studies have demonstrated beneficial treatment and prognostic factors in various markers. Whereas most previous reports focused on genomics proteomics, few lipidomics. With the advent mass spectrometry (MS), it has become possible to identify many types molecules, this analytical tool critical field omics. Recently, imaging (IMS) was developed. After a simple pretreatment process,...
Radiotherapy is one of the most effective treatments for head and neck cancer. However, development dry mouth syndrome an unavoidable side effect because, in addition to tumor, normal salivary glands are included irradiation field. Previously, we investigated protective efficacy basic fibroblast growth factor (bFGF) radiation-damaged glands. In this study, adipose-derived stromal cell (ADSC) transplantation regeneration radiation damaged glands.Prospective animal experiment with...
Loss of sensory hair cells in the inner ear is a major cause permanent hearing loss, since regeneration rarely occurs mammals. The aim this study was to examine potential neural stem cell transplantation restore mice. Fetal were transplanted into mouse after drug-induced injury. Histological analysis demonstrates that majority grafted differentiated glial or ear. Strikingly, however, we show integrate vestibular epithelia and express specific markers for cells. This finding suggests have...
Objectives: Vocal fold scarring is the major cause of voice disorders after surgery or laryngeal trauma. The role inflammatory factors in vocal wound healing and fibrosis has not been adequately investigated. Scarless associated with decreased responses. To understand scar formation develop reliable treatments, it necessary to control extracellular matrix production inflammation. Thus, we examined inflammation profile wounded folds acute phase healing. Methods: stripping was performed on 30...
Objectives: We used an acute vocal fold injury in a rat model to characterize wound healing by studying the expression pattern of extracellular matrix components lamina propria. Methods: Vocal stripping was performed unilaterally 27 Sprague-Dawley rats. The folds were harvested at 5 time points (1, 3, 5, 7, and 14 days) histologically analyzed Alcian blue stain, trichrome immunofluorescence with antibodies collagen type I, III, fibronectin. Results: Re-epithelialization occurred day 3...
Therapeutic challenges exist in the management of vocal fold scarring. We have previously demonstrated therapeutic potential hepatocyte growth factor (HGF) acute phase scarring using a novel hydrogel-based HGF drug delivery system (DDS). However, effect on matured remains unclear. The current study aims to investigate HGF-DDS chronic canine model.Animal model.Vocal folds from eight beagles were unilaterally scarred by stripping entire layer lamina propria; contralateral kept intact as normal...
Abstract Objectives/Hypothesis: Aged vocal folds are characterized by atrophy of the mucosa, which causes mucosal wave deficiency and glottal insufficiency. This clinical trial examined regenerative effects safety considerations basic fibroblast growth factor (bFGF) on restoration aged folds. Study Design: Institutional review board–approved human trial. Methods: Ten patients (6 men 4 women; mean age, 70.1 years) were recruited in micrograms bFGF was injected into each treated fold....
Mechanosensory hair cells and supporting develop from common precursors located in the prosensory domain of developing cochlear epithelium. Prosensory cell differentiation into or proceeds basal to apical region cochleae, but mechanism significance this basal-to-apical wave remain be elucidated. Here, we investigated role Hedgehog (Hh) signaling development by examining effects up- downregulation Hh vivo. The effector smoothened (Smo) was genetically activated inactivated specifically...
Vocal fold scar and sulcus are intractable diseases with no effective established treatments. Hepatocyte growth factor (HGF) has preclinically proven to have potent antifibrotic regenerative effects on vocal scar. The current Phase I/II clinical trial aims examine the safety effectiveness of intracordal injection a recombinant human HGF drug for patients or sulcus. This is an open-label, dose-escalating, first-in-human trial. Eighteen bilateral were enrolled divided into three groups: Step I...
In the auditory system, efforts to reduce degeneration of spiral ganglion neurons have immediate objective improving clinical benefits cochlear implants, which are small devices designed stimulate electronically. Recent studies indicated several neurotrophins can enhance survival neurons. However, strategy for application in inner ear is still a matter debate. this study, we examined potential cell therapy as ear. Neural stem cells obtained from green fluorescent protein-transgenic mice were...
Aged vocal folds have been reported to dense collagen deposition and decreased hyaluronic acid (HA) in the lamina propria. These characteristics are thought contribute problems that occur with age (presbyphonia). To restore better viscoelasticity aged folds, an intervention might increase HA decrease production from fold fibroblasts would appear be a potentially useful approach. Our previous vitro study has revealed basic fibroblast growth factor (bFGF) consistently stimulates decreases rat...
Purpose To develop and evaluate a rat excised larynx model for the measurement of acoustic, aerodynamic, vocal fold vibratory changes resulting from scar. Method Twenty-four 4-month-old male Sprague–Dawley rats were assigned to 1 4 experimental groups: chronic scar, scar treated with 100-ng basic fibroblast growth factor (bFGF), saline (sham treatment), unscarred untreated control. Following tissue harvest, histological immunohistochemical data collected confirm extracellular matrix...
Objectives/Hypothesis Vocal fold scar and sulcus are still challenges. Basic fibroblast growth factor (bFGF) has proven to be effective resolve tissue in animal models. This study reports the efficacy of regenerative treatments using bFGF on vocal human cases. Study Design Retrospective chart review. Methods Fifteen cases (7 scar; 8 sulcus) were treated by either local injection (n = 6) or surgery 9). Injection regimen was locally apply 10 micrograms 0.5 mL saline into each under topical...
Objectives/Hypothesis Vocal fold scarring presents therapeutic challenges. Recently, cell therapy with mesenchymal stromal cells has become a promising approach. The aim of this study was to compare the potential adipose-derived stem (ASC) bone marrow-derived (BMSC) for vocal regeneration. Study Design Prospective animal experiments controls. Methods folds Sprague-Dawley rats were unilaterally injured. Two months after injury, treated local injection ASC (ASC group), BMSC (BMSC or saline...
Objectives/Hypothesis To examine if macroscopic classification with a magnifying gastrointestinal endoscope narrow band imaging (ME‐NBI) is useful in predicting pathological depth of tumor invasion laryngo‐pharyngeal cancer. Study Design Retrospective study. Methods Preoperative endoscopy reports and postoperative on 139 cancer lesions were retrospectively reviewed, the association between findings was analyzed statistically. Results The ratios macroscopically classified as 0‐I (superficial...
Recently, a global phase III study demonstrated that nivolumab markedly improved patient outcomes in recurrent or metastatic head and neck carcinoma (RMHNC). However, the efficacy of patients who are ineligible for clinical trials is unknown. We investigated real-world prognostic factors associated with response to nivolumab. This was conducted at 11 institutes Kyoto University its Affiliated Hospitals-Head Neck Oncology Group. In total, 93 RMHNC received between May 2017 2018 were...
Objectives Inflammatory factors are key mediators of wound healing processes following injury, and their modulation may improve outcomes. The objective this study was to characterize in vivo inflammatory factor extracellular matrix (ECM) messenger RNA (mRNA) expression levels 1 hour after vocal fold injury. Methods Five Sprague-Dawley rats were subjected bilateral 5 reserved as uninjured controls, rat unilateral injury for histology. Tissue harvested Real-time reverse...