Glutamine metabolism plays an essential role in cell growth, and glutamate dehydrogenase (GDH) is a key enzyme. GDH promotes the of glutamine to generate ATP, which profoundly increased multiple human cancers. Through vitro vivo experiments, we verified that small-molecule inhibitor EGCG slowed progression fibrosis by inhibiting enzyme activity metabolism. SIRT4 mitochondrial with NAD ADP ribosylation downregulates activity. The liver related mechanisms are unknown. In this study, measured...

Senescence of activated hepatic stellate cells (HSCs) is crucial for the regression liver fibrosis. However, impaired immune clearance can result in accumulation senescent HSCs, exacerbating The activation cyclic GMP-AMP synthase-stimulator interferon genes (cGAS-STING) pathway essential both senescence and innate response. Additionally, specific delivery to HSCs hindered by their inaccessible anatomical location, capillarization sinusoidal endothelial (LSECs), loss substance exchange....

Abstract Background and Aim: Hepatic encephalopathy (HE) is a serious complication of decompensated liver cirrhosis, affecting the prognosis patients underwent transjugular intrahepatic portosystemic shunts (TIPS). We aim to create nomogram predict hepatic encephalopathy- free survivals (HEFS) after TIPS in cirrhotic select appropriate candidates for TIPS. Methods: Cirrhotic from 2015 2018 our department were included. Multivariable Cox regression was conducted estimate predictors overt HE...

Background The intestinal-liver axis is associated with various liver diseases. Here, we verified the role of gut microbiota and macrophage activation in progression pyrrolizidine alkaloids-induced hepatic sinusoidal obstruction syndrome (PA-HSOS), explored possible mechanisms new treatment options.

The aim of this study was to examine the effect diabetes mellitus (DM) on rate hepatic encephalopathy (HE) in patients with decompensated liver cirrhosis after creation a transjugular intrahepatic portosystemic shunt (TIPS).This retrospectively reviewed 436 consecutive receiving TIPS our department from 2008 2016. By comparing two groups patients, or without DM, incidence developing overt HE TIPS, as well correlation between and HE, analyzed. Data were analyzed using χ-tests, unpaired...

Transjugular intrahepatic portosystemic shunt (TIPS) is an effective intervention for portal hypertensive complications in cirrhosis. Spontaneous shunts (SPSSs) may increase the risk of post-TIPS and mortality. This study was done to evaluate safety efficacy TIPS treating variceal bleeding between patients with without SPSSs.The clinical data 467 consecutive cirrhosis who received from January 2012 2018 were screened. A total 33 coexisting SPSSs included as SPSS group, randomly selected...

Activated human hepatic stellate cells (HSCs) showed enhanced ability of migration compared with quiescent HSCs, which is pivotal in liver fibrogenesis. The aim the present study was to investigate effects tumor necrosis factor-like weak inducer apoptosis (TWEAK) on activated HSCs and explore relevant potential mechanisms. Human LX-2 were cultured TWEAK. TNFRSF12A-downexpressing lentiviruses used infect cells. specific matrix metalloproteinases inhibitor BB94, Src family kinase inhibitor,...

The study is to verify whether Calcitonin gene related peptide (CGRP) gene-modified rat bone mesenchymal stem cells (BMSCs) are promising seed with great potential repair defect. A recombinant lentiviral vector overexpressing CGRP peptide, pLenO-DCE-CGRP, was constructed and transfected into rBMSCs obtain stable expression. Differently rBMSCs: CGRP- modified (CGRP group), empty vector- (Vector group) normal (Control were transferred collagen scaffold skull defects (n=6). Skull specimens...

The purpose of this study was to investigate the effect body mass index (BMI) on prevalence overt hepatic encephalopathy (OHE) after transjugular intrahepatic portosystemic shunt (TIPS) procedure in decompensated cirrhotic patients. A retrospective observational cohort 145 patients receiving TIPS carried out our department from 2017 2020. relationships between BMI and clinical outcomes including OHE, as well risk factors developing post-TIPS were analyzed. categorized normal weight (18.5 ≤ <...

Background: Wedge hepatic vein pressure (WHVP) accurately estimates the portal (PP) in chronic sinusoidal hypertension patients. Whether this applies to patients with acute due obstruction syndrome (HSOS) is unclear. Our aim was assess agreement between WHVP and PP HSOS by comparing them decompensated cirrhosis Methods: From December 2013 2021, pyrrolidine alkaloid-induced (PA-HSOS) receiving venous gradient (HVPG) measurement transjugular intrahepatic portosystem shunt (TIPS) were...

Transjugular intrahepatic portosystemic shunt (TIPS) is a well-established procedure for treating complications of portal hypertension. Due to the complexity anatomy and difficulty puncture technique, itself might brought potential complications, such as failure, bleeding, infection, and, rarely, death. The aim this study explore incidence, management, outcome TIPS procedure-related major using covered stents.Patients who underwent implantation from January 2015 December 2020 were recruited...

Objectives. Transjugular intrahepatic portosystemic shunt (TIPS) is an effective intervention for portal hypertensive complications. Little known about the ability of spleen stiffness (SS) predicting survival cirrhotic patients undergoing TIPS. This study to evaluate influence SS detected by point shear wave elastography (pSWE) in after Methods. retrospective cohort screened consecutive who underwent TIPS and reliable pSWE measurement between October 2014 September 2017 from our...

Objective Propranolol is always titrated to the maximum tolerated dose prevent gastroesophageal variceal bleeding. However, some patients do not achieve a hemodynamic response and experience more intolerance discontinuation. This study evaluated dose-dependent effect of propranolol on tolerance in cirrhotic patients. Patients methods retrospective included 95 consecutive recruited from our prospective database. After hepatic venous pressure gradient measurement, received 10 mg, twice daily...

Background and objective Currently, monitoring hepatic venous pressure gradient (HVPG) have been proved to be the best predictor for risk of variceal bleeding. We performed study evaluate effect endoscopic therapy + β-blocker vs. covered transjugular intrahepatic portosystemic shunt (TIPS) prevention rebleeding in cirrhotic patients with HVPG ≥16 mmHg. Methods Consecutive mmHg treated or TIPS bleeding were retrospectively gathered between April 2013 December 2018. The rate, survival,...

Purpose The liver stiffness- spleen diameter to platelet ratio score (LSPS model) can identify a high risk of decompensated events in cirrhotic patients. We aimed evaluate the value LSPS model as stratification strategy secondary prevention for patients with esophageal and gastric variceal bleeding (EGVB). Methods Consecutive EGVB who underwent stiffness measurement by acoustic radiation force impulse, count ultrasonography were enrolled between January 2013 December 2019. calculated all...

To explore whether transjugular intrahepatic portosystemic shunt (TIPS) can improve the prognosis of esophagogastric variceal bleeding (EGVB) combined with sarcopenia in cirrhotic patients.

It is controversial whether transjugular intrahepatic portosystemic shunt (TIPS) placement can improve long-term survival.

Risk stratification to identify patients with high risk of variceal rebleeding is particularly important in decompensated cirrhosis. In clinical practice, eliminating gastroesphageal varices thoroughly after sequential endoscopic treatment reduces the rate, however, no simple method has been build predict rebleeding. We conducted this study explore value number sessions required eradicate identifying rebleeding.Consecutive cirrhotic received therapy between January 2015 and March 2020 were...

Abstract Background and aims: Risk stratification to identify patients with high risk of variceal rebleeding is particularly important in decompensated cirrhosis. In clinical practice, eliminating gastroesphageal varices thoroughly after sequential endoscopic treatment reduces the rate, however, no simple method has been build predict rebleeding. We conducted this study explore value number sessions required eradicate identifying Patients methods: Consecutive cirrhotic received therapy...

Abstract Objectives: TIPS can decrease rebleeding in cirrhotic patients with variceal bleeding (VB), but it is controversial whether improve survival. We aimed to assess improves survival selected based on HVPG-related risk stratification a long-term follow-up. Methods: Consecutive VB treated endoscopic therapy+NSBBs or covered were retrospectively enrolled between January 2013 and December 2019. All had HVPG measurements by experienced personnel before therapy. Outpatient follow-up was...

Abstract Glutamine metabolism plays an essential role in cell growth. Glutamate dehydrogenase (GDH) is a key enzyme glutamine metabolism, promoting the of glutamate and generating ATP, level which profoundly increased multiple human cancers. Through vitro vivo experiments, we verified that small-molecule GDH inhibitor EGCG slows progression fibrosis by inhibiting activity metabolism. SIRT4 mitochondrial with NAD promotes ADP-ribosylation downregulates activity. The played liver related...