A5037240639- Influenza Virus Research Studies
- Respiratory viral infections research
- SARS-CoV-2 and COVID-19 Research
- SARS-CoV-2 detection and testing
- COVID-19 Clinical Research Studies
- Monoclonal and Polyclonal Antibodies Research
- Viral gastroenteritis research and epidemiology
- Animal Disease Management and Epidemiology
- Mosquito-borne diseases and control
- Immune Response and Inflammation
- Malaria Research and Control
- Viral Infections and Immunology Research
- Vibrio bacteria research studies
- Long-Term Effects of COVID-19
- Herpesvirus Infections and Treatments
- Viral Infections and Outbreaks Research
- Transgenic Plants and Applications
- Virus-based gene therapy research
- Vaccine Coverage and Hesitancy
- Cytomegalovirus and herpesvirus research
- Receptor Mechanisms and Signaling
- Virology and Viral Diseases
- COVID-19 epidemiological studies
- Immunotherapy and Immune Responses
- Biosensors and Analytical Detection
VisMederi Research
2018-2024
Serum Institute of India (India)
2022
Syngene International (India)
2022
University of Siena
2009-2014
The recent swine H1N1 influenza outbreak demonstrated that egg-based vaccine manufacturing has an Achille's heel: its inability to provide a large number of doses quickly. Using novel platform based on transient expression surface glycoproteins in Nicotiana benthamiana, we have recently candidate Virus-Like Particle (VLP) can be generated within 3 weeks release sequence information. Herein report alum-adjuvanted plant-made VLPs containing the hemagglutinin (HA) protein H5N1...
A newly identified coronavirus, named SARS-CoV-2, emerged in December 2019 Hubei Province, China, and quickly spread throughout the world; so far, it has caused more than 49.7 million cases of disease 1,2 deaths. The diagnosis SARS-CoV-2 infection is currently based on detection viral RNA nasopharyngeal swabs by means molecular-based assays, such as real-time RT-PCR. Furthermore, serological assays detecting different classes antibodies constitute an excellent surveillance strategy for...
SARS-CoV-2 pandemic is causing high morbidity and mortality burden worldwide with unprecedented strain on health care systems. To investigate the time course of antibody response in relation to outcome we performed a study hospitalized COVID-19 patients. As comparison also investigated asymptomatic subjects. Study results show that patients produce strong correlation between different viral antigens (spike protein nucleoprotein) among classes (IgA, IgG, IgM neutralizing antibodies). The peak...
Children are an important target group for influenza vaccination, but few studies have prospectively evaluated vaccine efficacy (VE) in children under 3 years of age. This was a randomized Phase III trial to assess the efficacy, immunogenicity, and safety inactivated quadrivalent (QIV) young (EudraCT: 2016–004904–74). Influenza-naïve aged 6–35 months were during three seasons receive vaccination with QIV or non-influenza control vaccine. One participants revaccinated subsequent season. The...
Please cite this paper as: Svindland et al. (2012) A study of Chitosan and c‐di‐GMP as mucosal adjuvants for intranasal influenza H5N1 vaccine. Influenza Other Respiratory Viruses 10.1111/irv.12056000(000), 000–000. Background Highly pathogenic avian A/H5N1 virus remains a potential pandemic threat, it is essential to continue vaccine development against subtype. local immune response in the upper respiratory tract may stop transmission. It therefore important develop effective vaccines that...
We investigated the protective efficacy of two intranasal chitosan (CSN and TM-CSN) adjuvanted H5N1 Influenza vaccines against highly pathogenic avian (HPAI) intratracheal challenge in a ferret model. Six groups 6 ferrets were intranasally vaccinated twice, 21 days apart, with either placebo, antigen alone, CSN antigen, or TM-CSN antigen. Homologous intra-subtypic antibody cross-reacting responses assessed. Ferrets inoculated intratracheally (all treatments) placebo treatments only) clade 1...
The haemagglutination inhibition assay (HAI) and the virus microneutralisation (MN) are long-established methods for quantifying antibodies against influenza viruses. Despite their widespread use, both assays require standardisation to improve inter-laboratory agreement in testing. FLUCOP consortium aims develop a toolbox of standardised serology seasonal influenza. Building upon previous collaborative studies harmonise HAI, this study carried out head-to-head comparison harmonised HAI MN...
Egg-derived viruses are the only available seed material for influenza vaccine production. Vaccine manufacturing is done in embryonated chicken eggs, MDCK or Vero cells. In order to contribute efficient production of vaccines, we investigate whether quality inactivated vaccines influenced by propagation substrate. We demonstrate that H3N2 egg-derived (A/Brisbane/10/07, IVR147, and A/Uruguay/716/07) triggered hemagglutinin (HA) conformational change under less acidic conditions (0.2-0.6 pH...
Influenza vaccination remains the best strategy for prevention of influenza virus-related disease and reduction severity mortality. However, there is large individual variation in vaccine responses. In this study, we investigated effects gender, age, underlying diseases, medication on responses 1,852 Icelanders broad age range who received trivalent inactivated virus 2012, 2013, or 2015. Hemagglutination inhibition (HAI) microneutralization (MN) titers were measured pre- post-vaccination...
To analyse the immunogenicity and safety of inactivated subunit quadrivalent influenza vaccine (QIV) versus trivalent (TIV) in children adolescents 3-17 years age.In this phase III, multicentre, double-blind study, 1200 subjects were randomized to receive QIV (n=402), TIV with B-strain Victoria lineage (n=404), or Yamagata (n=394). The primary objective was demonstrate non-inferiority for against shared strains, based on post-vaccination hemagglutinin inhibition (HI) titres. Secondary...
The neutralization assays are considered the gold-standard being capable of evaluating and detecting, functional antibodies. To date, many different protocols exist for micro-neutralization (MN) assay which varies in several steps: cell number seeding conditions, virus amount used infection step, virus-serum-cells incubation time read out. aim present preliminary study was to carry out SARS-CoV-2 wild type MN order investigate optimal tissue culture infective dose 50 (TCID50) use is most...
Pandemic H1N1 influenza vaccine antigens are currently being manufactured. The MF59 adjuvant has an established safety profile in humans and a proven ability to increase responses some vaccines humans. To inform initial decisions on the use of these components protect human populations, we have immunized mice with MF59-adjuvanted or non-adjuvanted pandemic vaccine. Immunizing unprimed single dose elicits functional antibody titers equivalent those associated protection from seasonal...
Formulation of neuraminidase (NA) within influenza vaccines is gaining importance in light recent human studies. The enzyme-linked lectin assay (ELLA) considered a reliable to evaluate anti-NA antibodies.To overcome interference by hemagglutinin (HA)-specific antibodies and detect inhibitory (NI) only, two different sources antigen have been studied ELLA: reassortant viruses with mismatched avian origin-HA or Triton X-100 (Tx)-treated wild-type viruses. Pseudotypes pseudovirus (PV),...
ABSTRACT A newly identified coronavirus, named SARS-CoV-2, emerged in December 2019 Hubei Province, China, and quickly spread throughout the world; so far, it has caused more than 18 million cases of disease 700,000 deaths. The diagnosis SARS-CoV-2 infection is currently based on detection viral RNA nasopharyngeal swabs by means molecular-based assays, such as real-time RT-PCR. Furthermore, serological assays aimed at detecting different classes antibodies constitute best surveillance...
Previous exposures to flu and subsequent immune responses may impact on 2009/2010 pandemic vaccine clinical symptoms upon infection with the 2009 H1N1 influenza strain. Qualitative quantitative differences in humoral cellular associated vaccination (pandemic vaccine) natural have not yet been described detail. We designed a longitudinal study examine influenza- (flu-) specific association between pre-existing responses, of influenza-like illness (ILI), infection, cohort 2,040 individuals...
ABSTRACT SARS-CoV-2 pandemic is causing high morbidity and mortality burden worldwide with unprecedented strain on health care systems. To elucidate the mechanism of infection, protection, or rapid evolution until fatal outcome disease we performed a study in hospitalized COVID-19 patients to investigate time course antibody response relation outcome. In comparison investigated asymptomatic subjects. Study results show that produce strong correlation between different viral antigens (spike...
The human population is constantly exposed to multiple influenza A subtypes due zoonotic spillover and rapid viral evolution driven by intrinsic error-prone replication immunological pressure. In this context, antibody responses directed against the HA protein are of importance since they have been shown correlate with protective immunity. Serological techniques, detecting these responses, play a critical role for surveillance, vaccine development, assessment. As recent pandemics avian...
Yellow fever, a mosquito-borne flavivirus infection, is an important public health problem in Africa and Latin America. A Fever vaccine (YFV) was developed tested study India. This Phase I, open-label, randomized, controlled where healthy adults received SII YFV intramuscularly (SII IM), subcutaneously SC) or STAMARIL® (Sanofi-Pasteur) 1:1:1 ratio. They were followed for solicited reactions 10 days unsolicited events 28 serious adverse 3 months. YF-neutralizing antibodies measured at...
Yellow fever (YF) is a mosquito-borne viral disease that endemic to sub-Saharan Africa and tropical South America. Although there safe effective vaccine has been available for over 75 years, YF remains public health problem in areas with sporadic transmission. Immune responses elicited by natural infection yellow virus vaccination are marked production of neutralizing antibodies. Serological cross-reactivity exhibited flaviviruses poses challenges the diagnostic tests detection YF-specific...
Abstract Due to the global spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), there is an urgent need for reliable high-throughput serological assays in order evaluate immunological responses against SARS-COV-2 virus and enable population screening, as well vaccines drug’s efficacy testing. Several SARS-CoV-2 are now becoming available market. However, it has also become extremely important have well-established with desirable high sensitivity specificity. To date,...
Abstract Background Yellow fever, a mosquito borne flavivirus infection, is an important public health problem in Africa and Latin America. A Fever vaccine (YFV) was developed tested study India. Methods This Phase I, open-label, randomized, controlled where healthy adults received SII YFV intramuscularly (SII IM), subcutaneously SC) or STAMARIL® (Sanofi-Pasteur) 1:1:1 ratio. They were followed for solicited reactions 10 days unsolicited events 28 serious adverse 3 months. YF neutralizing...