Advanced cutaneous T-cell lymphoma (CTCL) is resistant to chemotherapy and presents a major area of medical need. In view the known role microRNAs (miRNAs) in regulation cellular signalling, we aimed identify functionally important miRNA species, which regulate apoptosis CTCL. Using recently established model CTCL cell lines induced by Notch-1 inhibition γ-secretase inhibitors (GSIs), found that miR-122 was significantly increased apoptotic cells. up-regulation not specific for GSI-1 but...

Daylight-mediated photodynamic therapy (daylight PDT) is a simple and pain free treatment of actinic keratoses. Weather conditions may not always allow daylight PDT outdoors. We compared the spectrum five different lamp candidates for indoor "daylight PDT" investigated their ability to photobleach protoporphyrin IX (PpIX). Furthermore, we measured amount PpIX activating available in glass greenhouse, which can be an alternative when it uncomfortable patients The lamps were: halogen (overhead...

Skin pretreatment is recommended for adequate penetration of topical photosensitizing agents and subsequent protoporphyrin IX (PPIX) accumulation in photodynamic therapy (PDT).To compare the relative potential different physical pretreatments to enhance PPIX fluorescence normal skin.This intraindividual, randomized clinical trial was performed from November 28 December 20, 2014, at Bispebjerg Hospital, Copenhagen, Denmark, among 12 healthy volunteers 18 years or older. Analysis based on...

Background and Objective Ablative fractional laser (AFXL) facilitates delivery of topical methotrexate (MTX). This study investigates impact laser-channel depth on MTX-delivery. Materials Methods MTX (1% [w/v]) diffused for 21 hours through AFXL-exposed porcine skin in vitro Franz Cells (n = 120). A 2,940 nm AFXL generated microscopic ablation zones (MAZs) into epidermis (11 mJ/channel, MAZ-E), superficial-dermis (26 MAZ-DS), mid-dermis (256 MAZ-DM). High performance liquid chromatography...

Tattoo pigments are deposited in the skin and known to distribute regional lymph nodes. small particles may be hypothesized reach blood stream become distributed peripheral organs. This has not been studied past. The aim of study was trace tattoo internal organs mice extensively tattooed with 2 different ink products.Three groups were studied, i.e., 10 black, red, 5 untreated controls. They on entire back commercial inks, black red. Mice sacrificed after 1 year. Samples isolated from skin,...

Objectives: Methotrexate (MTX) is a chemotherapeutic and anti-inflammatory drug that may cause systemic adverse effects. This study investigated kinetics biodistribution of MTX delivered topically by ablative fractional laser (AFXL).Methods: In vitro passive diffusion 10 mg/ml (1 w/v%) was measured from 0.25 to 24 h through AFXL-processed intact porcine skin in Franz Cells (n = 46). A 2,940 nm Erbium Yttrium Aluminium Garnet generated mid-dermal microchannels at 2.4% density, 256...

Micro RNAs (miRs) have emerged as key regulators during oncogenesis. They been found to regulate cell proliferation, differentiation, and apoptosis. Mir-125b has identified an oncomir in various forms of tumours, but we previously proposed that miR-125b is a suppressor lymph node metastasis cutaneous malignant melanoma. Our goal was therefore further examine this theory.We used in-situ-hybridization visualise expression primary tumours metastasis. Then using miRVector plasmid containing...

Abstract This study aimed to investigate the impact of ablative fractional laser (AFL) on hedgehog pathway gene expression in murine microscopic basal cell carcinomas (BCCs) and compare these results effect topical treatment with vismodegib, an FDA-approved inhibitor. In 25 mice, 1 cm 2 skin test sites ( n = 44) containing BCCs were exposed one three interventions: a single CO AFL (1 pulse, 40 mJ/microbeam, wavelength 10.6 μm, 5% density, pulse rate 250 Hz, 12), eight vismodegib treatments...

Black tattoos may involve risk of cancer owing to polycyclic aromatic hydrocarbons including benzo(a)pyrene (BaP) in inks. Ultraviolet radiation (UVR) induces skin cancer. The combination UVR and black tattoo therefore potentially be very problematic, but has not been previously studied.Immunocompetent C3.Cg/TifBomTac mice (n = 99) were tattooed on the back with Starbrite Tribal Black(™) . This ink a high content carcinogen BaP. Half irradiated three standard erythema doses thrice weekly....

Background and Objective Hedgehog inhibitors such as vismodegib are targeted drugs widely used for the treatment of basal cell carcinomas; however, their use is significantly limited by frequent systemic side effects due to oral administration route. We aim ablative fractional laser (AFL) enable topical delivery relevant dermal depths. Materials Methods Pig skin was treated in vitro with a 10,600 nm CO 2 at 0 or 80 mJ/microbeam exposed (6.4 mmol/L) Franz‐diffusion cells 0.5, 4, 24 hours ( n...

Bleomycin exhibits antiproliferative effects desirable for use in dermato-oncology but topical is limited by its 1415 Da molar mass. Ablative fractional laser (AFL)-assisted drug delivery has been shown to enhance uptake skin. The aim of this study was with AFL deliver bleomycin into skin, quantify uptake, and visualize biodistribution mass spectrometry. In a Franz diffusion cell study, pig skin samples (n = 66) were treated (λ 10,600 nm), 5% density, 0, 5, 20, or 80 mJ/microbeam (mb) pulse...

Systemic chemotherapy with the anticancer agent cisplatin is approved for advanced non-melanoma skin cancer (NMSC), but topical treatment limited by insufficient cutaneous penetration. We studied impact of ablative fractional laser (AFL) exposure on cisplatin's pharmacokinetics and biodistribution in skin, using microscopic ablation zones reaching mid- (MAZ-MD; 620 μm depth) deep dermis (MAZ-DD; 912 (λ = 10,600 nm, 196 MAZ/cm2). Assessed an vitro Franz cell model after 0.5-, 4-, 24 h (n 8),...

Cutaneous squamous cell carcinoma (SCC) is an increasingly prevalent global health concern. Current diagnostic and surgical methods are reliable, but they require considerable resources do not provide metabolomic insight. Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) enables detailed, spatially resolved analysis of tissue samples. Integrated with machine learning, MALDI-MSI could yield detailed information pertaining to the metabolic alterations...

Hexyl aminolevulinate (HAL) is a long-chained 5-aminolevulinic acid-ester that has been proposed as novel photosensitizing agent to methyl (MAL) in topical photodynamic therapy (PDT). The more lipophilic HAL, may improve treatment outcome for non-melanoma skin cancer.To compare the prophylactic and therapeutic effects of HAL- MAL-PDT ultraviolet-induced squamous cell carcinomas (SCCs) hairless mice.Mice (n = 249) were irradiated with solar UV-radiation (UVR) until SCC occurred. Before any...

Abstract Ultraviolet radiation (UVR) induces skin cancer. The combination of UVR and red tattoos may be associated with increased risk cancer due to potential carcinogens in tattoo inks. This has not been studied previously. Immunocompetent C3.Cg/TifBomTac hairless mice (n=99) were tattooed on their back a popular ink. often used ink is banned for use humans because high content the carcinogen 2‐anisidine. Half irradiated three standard erythema doses thrice weekly. Time induction first,...

Background and aims: Pharmacovigilance reports, associating hydrochlorothiazide (HCT) with skin cancer, resulted in a significant decrease of HCT prescriptions for hypertension heart failure. Whether exhibits phototoxic properties thereby causing cancer remains unknown. This study aimed to examine the photosensitizing, carcinogenic potential randomized, placebo-controlled, double-blind trial vivo also vitro . Methods: The assigned 30 healthy, normotensive adult volunteers 2:1 ratio either 25...

Pimecrolimus and tacrolimus are topical calcineurin inhibitors developed specifically for the treatment of atopic eczema. Experience with long-term use is limited risk rare but serious adverse events remains a concern. We have previously demonstrated absence carcinogenic effect alone in combination simulated solar radiation (SSR) on hairless mice. The aim this study to determine whether pimecrolimus accelerates photocarcinogenesis SSR or accelerate UVA. used 11 groups 25 female...