A5078520843- Cancer, Lipids, and Metabolism
- Genetics, Aging, and Longevity in Model Organisms
- Metabolomics and Mass Spectrometry Studies
- Cancer, Hypoxia, and Metabolism
- Circadian rhythm and melatonin
- interferon and immune responses
- Diet and metabolism studies
- GABA and Rice Research
- Biochemical and biochemical processes
- Gut microbiota and health
- Adipose Tissue and Metabolism
- Mosquito-borne diseases and control
- Parkinson's Disease Mechanisms and Treatments
- Microbial Metabolic Engineering and Bioproduction
- Ferroptosis and cancer prognosis
- Invertebrate Immune Response Mechanisms
- Tryptophan and brain disorders
- Renal cell carcinoma treatment
Seoul National University
2020-2024
Max Planck Institute for Biology of Ageing
2024
Clear cell renal carcinoma (ccRCC), with its hallmark phenotype of high cytosolic lipid content, is considered a metabolic cancer. Despite the implication this lipid-rich in ccRCC tumorigenesis, roles and regulators de novo synthesis (DNL) remain largely unexplained.Our bioinformatic screening focused on ccRCC-lipid phenotypes identified glutathione peroxidase 8 (GPX8), as clinically relevant upstream regulator DNL. GPX8 genetic silencing was performed CRISPR-Cas9 or shRNA lines to dissect...
Despite the importance of mitochondrial fatty acid oxidation (FAO) in cancer metabolism, biological mechanisms responsible for FAO and therapeutic intervention based on catabolic metabolism are not well defined. In this study, we observe that Snail (SNAI1), a key transcriptional repressor epithelial–mesenchymal transition, enhances FAO, allowing pro-survival breast cells starved environment. Mechanistically, suppresses ACC2 (ACACB) by binding to series E-boxes located its proximal promoter,...
Gut microbiome can affect drug metabolism considerably, leading to modified response. However, quantitative estimation of host vs. microbial contributions in a living host–gut system has been challenging. Using the interspecies Caenorhabditis elegans and gut bacteria, we developed real-time approach for monitoring their metabolic interaction vivo during anticancer 5-fluorouracil (5-FU) metabolism. The fluorine NMR-based yielded 5-FU made by human gut-microbial species variable genetic...
AMP-activated protein kinase (AMPK in human and AAK C. elegans) is a master regulator of metabolism. It has many isotypes, but its isotype-dependent functions are largely unknown. By developing real-time in-organism NMR metabolomics for elegans, we were able to study different roles the isotypic catalytic subunits AAK/AMPK, AAK-1, AAK-2 live worms at whole organism level. The aak-1 knockout animals exhibited enhanced glucose production under starvation, strikingly opposite aak-2 animals....
The gut microbiome influences drug metabolism and therapeutic efficacy. Still, the lack of a general label-free approach for monitoring bacterial or host metabolic contribution hampers deeper insights. Here, 2D nuclear magnetic resonance (NMR) is introduced that enables real-time Levodopa (L-dopa), an anti-Parkinson drug, in both live bacteria bacteria-host (Caenorhabditis elegans) symbiotic systems. quantitative method reveals discrete Enterococcus faecalis substrains produce different...
Abstract The cGAS/STING pathway is a central innate immune signaling whose chronic activation has been implicated in numerous age-related pathologies, yet its impact on life span itself unknown. Here we engineered knockouts of this the killifish Nothobranchius furzeri , and assessed physiology aging. In vitro loss cGAS or STING mitigated DNA damage-induced senescence cultured fibroblasts. vivo knockout unexpectedly led to low-grade inflammation. It also attenuated changes gene expression...