A5025563270- Vitamin D Research Studies
- Bone health and osteoporosis research
- Pharmacological Effects and Toxicity Studies
- Parathyroid Disorders and Treatments
- Glycogen Storage Diseases and Myoclonus
- Diet and metabolism studies
- Protein Tyrosine Phosphatases
- Enzyme Structure and Function
- Folate and B Vitamins Research
- Electrolyte and hormonal disorders
- Cardiovascular Disease and Adiposity
- Healthcare cost, quality, practices
- Cystic Fibrosis Research Advances
- Diabetes Treatment and Management
- Air Quality and Health Impacts
- Heart Failure Treatment and Management
- Stress Responses and Cortisol
- Lysosomal Storage Disorders Research
- S100 Proteins and Annexins
- Neurological disorders and treatments
- Immune Response and Inflammation
- Pharmacogenetics and Drug Metabolism
- Diet, Metabolism, and Disease
- Family and Patient Care in Intensive Care Units
- Menstrual Health and Disorders
University of Washington
2019-2025
University of California, San Diego
2009-2017
Abstract Context A comprehensive characterization of racial/ethnic variations in vitamin D metabolism markers may improve our understanding differences bone and mineral homeostasis the risk D–related diseases. Objective Describe their associations with genetic ancestry. Design, Setting, Participants In a cross-sectional study within Multi-Ethnic Study Atherosclerosis (MESA), we compared panel across self-reported groups Black (N = 1759), White 2507), Chinese 788), Hispanic 1411). We...
Macrophages detect pathogen infection via the activation of their plasma membrane-bound Toll-like receptor proteins (TLRs). The heterotypic interaction between Toll/interleukin-1 (TIR) domains TLRs and adaptor proteins, like Myeloid differentiation primary response gene 88 (MyD88), is first intracellular step in signaling pathway mammalian innate immune response. hetero-oligomerization TIRs brings about transcription factor NF-κB, which regulates synthesis pro-inflammatory cytokines. Here,...
Abstract Fracture risk is high in chronic kidney disease (CKD) and underlying pathophysiology factors may differ from the general population. In a cohort study of 3939 participants renal insufficiency (CRIC), we used Cox regression to test associations putative with composite first hip or vertebral fracture assessed using hospital discharge codes. Mean age was 58 years, 45% were female, 42% Black, 13% Hispanic. There 82 24 fractures over mean (SD) 11.1 (4.8) years (2.4 events per 1000...
Abstract Context The response to treatment with vitamin D varies between patients. Objective To identify genetic variants associated the biochemical D3 supplementation. Design Randomized placebo-controlled trial conducted 2017 and 2019. Setting was nested in an ongoing community-based cohort study, Multi-Ethnic Study of Atherosclerosis (MESA). Intervention 2,000 International Units or placebo daily for 16 weeks. Participants analytic sample included 427 participants assigned (mean age 73 y,...
Background: Kidney failure and its treatments disrupt glucose homeostasis in ways that may promote both hyperglycemia hypoglycemia. Continuous monitoring (CGM) delineates detailed glycemic profiles, but published studies kidney are limited to small, select groups. We aimed characterize the spectrum of glycemia determinants a large, diverse maintenance dialysis population. Methods: conducted prospective community-based cohort study people treated with dialysis. Each participant wore Dexcom G6...
Crystal structures of the xenobiotic metabolizing cytochrome P450 2B4 have demonstrated markedly different conformations in presence imidazole inhibitors or absence ligand. However, knowledge plasticity enzyme solution has remained scant. Thus, hydrogen-deuterium exchange mass spectrometry (DXMS) was utilized to probe ligand-free and complex with 4-(4-chlorophenyl)imidazole (4-CPI) 1-biphenyl-4-methyl-1H-imidazole (1-PBI). The results DXMS indicate that binding 4-CPI slowed rate over B'-...
Laforin and starch excess 4 (SEX4) are founding members of a class phosphatases that dephosphorylate phosphoglucans. Each protein contains carbohydrate binding module (CBM) dual-specificity phosphatase (DSP) domain. The gene encoding laforin is mutated in fatal neurodegenerative disease called Lafora (LD). In the absence function, insoluble glucans hyperphosphorylated exhibit sparse branching accumulate. It hypothesized these accumulations trigger neurodegeneration premature death LD...
X-ray crystallography provides excellent structural data on protein–DNA interfaces, but crystallographic complexes typically contain only small fragments of large DNA molecules. We present a new approach that can use longer substrates and reveal interactions even in extensively studied systems. Our combines rigid-body computational docking with hydrogen/deuterium exchange mass spectrometry (DXMS). DXMS identifies solvent-exposed protein surfaces; is used to create 3-dimensional model the...
Protein-protein interactions are essential for biological function, but structures of protein-protein complexes difficult to obtain experimentally. To derive the protein complex DNA-repair enzyme human uracil-DNA-glycosylase (hUNG) with its inhibitor (UGI), we combined rigid-body computational docking hydrogen/deuterium exchange mass spectrometry (DXMS). Computational unbound provides a list possible three-dimensional models complex; DXMS identifies solvent-protected residues. showed that...
Significance Statement The pharmacokinetic clearance of 25-hydroxyvitamin D (25[OH]D) is an essential, yet often overlooked, determinant the concentration circulating 25(OH)D, prevailing marker vitamin-D status. Observational studies have associated markers low 25(OH)D with poor clinical outcomes and suggest differences in by kidney function race. In this study, authors used gold-standard methods to show that reduced worsening eGFR. They also found that, among participants normal eGFR, but...
Abstract Context The effect of daily vitamin D supplementation on the serum concentration (the parent compound) may offer insight into disposition. Objective To assess total response to D3 and whether it varies according participant characteristics. compare results with corresponding for 25-hydroxyvitamin [25(OH)D], which is used clinically measured in trials. Design Exploratory study within a randomized trial. Intervention 2000 International Units per day (or matching placebo). Setting...
Background The ratio of 24,25‐dihydroxyvitamin D 3 /25‐hydroxyvitamin (vitamin metabolite [VDMR]) may reflect functional vitamin activity. We examined associations the VDMR, 25‐hydroxyvitamin (25[OH]D), and 1,25‐dihydroxyvitamin (1,25[OH] 2 D) with cardiovascular disease (CVD) in patients chronic kidney disease. Methods Results This study included longitudinal cross‐sectional analyses 1786 participants from CRIC (Chronic Renal Insufficiency Cohort) Study. Serum , 25(OH)D, 1,25(OH) were...
Studies on associations between biomarkers of vitamin D metabolism and fracture risk have focused predominantly White or elderly populations may not be generalizable to relatively healthy multiethnic populations. We tested total 25-hydroxyvitamin (25[OH]D), the ratio 24,25-dihydroxyvitamin