A5060336904- Epigenetics and DNA Methylation
- Extracellular vesicles in disease
- Amyotrophic Lateral Sclerosis Research
- Retinal Development and Disorders
- interferon and immune responses
- Cell Adhesion Molecules Research
- Immune responses and vaccinations
- SARS-CoV-2 and COVID-19 Research
- Cancer-related gene regulation
- Immune cells in cancer
- Mesenchymal stem cell research
- Retinopathy of Prematurity Studies
- Autism Spectrum Disorder Research
- Graphene and Nanomaterials Applications
- Renal Diseases and Glomerulopathies
- Advanced biosensing and bioanalysis techniques
- Peroxisome Proliferator-Activated Receptors
- Tissue Engineering and Regenerative Medicine
- Ubiquitin and proteasome pathways
- Connexins and lens biology
- Genomics and Rare Diseases
- Bacterial Infections and Vaccines
- Nuclear Receptors and Signaling
- Histone Deacetylase Inhibitors Research
- MicroRNA in disease regulation
Bilkent University
2019-2024
Johns Hopkins Medicine
2021-2023
Johns Hopkins University
2021-2023
German Center for Neurodegenerative Diseases
2023
Universität Ulm
2023
Radboud University Nijmegen
2010-2014
Radboud University Medical Center
2014
Non-healing wounds remain a major burden due to the lack of effective treatments. Mesenchymal stem cell-derived exosomes (MSC-Exo) have emerged as therapeutic options given their pro-regenerative and immunomodulatory features. Still, little is known on exact mechanisms mediated by MSC-Exo. Importantly, modulation efficacy through 3D-physiologic cultures together with loading strategies continues underexplored. To uncover MSC-Exo-mediated mechanism via proteomic analyses, use 3D-culture...
To determine the genetic defects underlying autosomal recessive retinitis pigmentosa (arRP) in Dutch population and a subset of patients originating from other countries. The hypothesis was that, because there has been little migration over past centuries certain areas Netherlands, significant fraction arRP carry their defect homozygous state.High-resolution genome-wide SNP genotyping on arrays subsequent homozygosity mapping were performed large cohort 186 mainly nonconsanguineous families...
Immunomodulatory commensal bacteria modify host immunity through delivery of regulatory microbial-derived products to cells. Extracellular membrane vesicles (MVs) secreted from symbiont commensals represent one such transport mechanism. How MVs exert their anti-inflammatory effects or whether tolerance-inducing potential can be used for therapeutic purposes remains poorly defined. In this study, we show that isolated the human lactic acid Pediococcus pentosaceus suppressed Ag-specific...
Bietti's crystalline dystrophy (BCD) is a rare, autosomal recessive retinal degenerative disease associated with mutations in CYP4V2. In this study, we describe the genetic and clinical findings 19 unrelated BCD patients recruited from five international clinics. Patients underwent ophthalmic examinations were screened for CYP4V2 by Sanger sequencing quantitative polymerase chain reaction (qPCR) copy number variation screening. Eight found 10/19 patients, including three whom only...
Abstract Invasive meningococcal disease (IMD) is caused by Neisseria meningitidis, with the main serogroups responsible for being A, B, C, W, X, and Y. To date, several vaccines targeting N. meningitidis have been developed albeit a short-lived protection. Given that MenW MenB are most common causes of IMD in Europe, Turkey, Middle East, we aimed to develop an outer membrane vesicle (OMV) based bivalent vaccine as heterologous antigen source. Herein, compared immunogenicity, breadth serum...
ALS is a fatal motor neuron disease that displays broad variety of phenotypes ranging from early courses to slowly progressing and rather benign courses. Such divergence can also be seen in genetic cases with varying bearing specific mutations, suggesting epigenetic mechanisms like DNA methylation act as modifiers. However, the epigenotype dictated by, addition other mechanisms, strongly influenced by individual's genotype. Hence, we performed study using EPIC arrays on 7 monozygotic (MZ)...
Abstract Epigenetic remodeling is emerging as a critical process for several neurodegenerative diseases, including amyotrophic lateral sclerosis (ALS). Genetics alone fails to explain the etiology of ALS, investigation epigenome might therefore provide novel insights into molecular mechanisms disease. In this study, we interrogated epigenetic landscape in peripheral blood mononuclear cells (PBMCs) familial ALS (fALS) patients with either chromosome 9 open reading frame 72 ( C9orf72 ) or...
Doxorubicin (Dox) is one of the most widely used treatments for breast cancer, although limited by well-documented cardiotoxicity and other off-target effects. Mesenchymal stem cell (MSC) secretome has shown immunomodulatory regenerative properties, further potentiated under 3D conditions. This work aimed to uncover effect MSC-derived from (CM3D) or 2D (CM2D) cultures, in human malignant cells (MDA-MB-231), non-tumor epithelial (MCF10A) differentiated AC16 cardiomyocytes, co-treated with...
Immune-mediated inflammation must be down-regulated to facilitate tissue remodeling during homeostatic restoration of an inflammatory response. Uncontrolled or over-exuberant immune activation can cause autoimmune diseases, as well destruction. A151, the archetypal example a chemically synthesized suppressive oligodeoxynucleotide (ODN) based on repetitive telomere-derived TTAGGG sequences, was shown successfully down-regulate variety responses. However, degree, duration and breadth...
Abstract The transcriptional repressor called parkin interacting substrate (PARIS; ZNF746) was initially identified as a novel co-substrate of and PINK1 that leads to Parkinson’s disease (PD) by disrupting mitochondrial biogenesis through peroxisome proliferator-activated receptor gamma (PPARγ) coactivator -1α (PGC-1α) suppression. Since its initial discovery, growing evidence has linked PARIS defective observed in PD pathogenesis. Yet, dopaminergic (DA) neuron-specific mechanistic...
Since previous research suggests a role of circulating factor in the pathogenesis steroid-sensitive nephrotic syndrome (NS), we speculated that plasma extracellular vesicles (EVs) are candidate source such soluble mediator. Here, aimed to characterize and try delineate effects these EVs vitro. Plasma from 20 children with NS relapse remission, 10 healthy controls, 6 disease controls were obtained by serial ultracentrifugation. Characterization was performed electron microscopy, flow...
Homeostatic restoration of an inflammatory response requires quenching the immune system after pathogen threats vanish. A continued assault orchestrated by host defense results in tissue destruction or autoimmunity. A151 is epitome synthetic oligodeoxynucleotides (ODNs) that curb a subset white corpuscles through repetitive telomere-derived TTAGGG sequences. Currently, genuine effect on cell transcriptome remains unknown. Here, we leveraged integrative approach where weighted gene...
Abstract Epigenetic remodeling is emerging as a critical process for several neurodegenerative diseases, including Amyotrophic Lateral Sclerosis (ALS). Genetics alone fails to explain the etiology of ALS, investigation epigenome might therefore provide novel insights into molecular mechanisms disease. In this study, we interrogated epigenetic landscape in peripheral blood mononuclear cells (PBMCs) familial ALS (fALS) patients with either chromosome 9 open reading frame 72 ( C9orf72 ) or...
The lower than expected number of SARS-CoV-2 cases in countries with fragile health systems is puzzling Herein, we hypothesize that BCG vaccination policies and vaccine strain preferences adopted by different might influence the transmission patterns and/or COVID-19 associated morbidity mortality We also postulate until a specific developed, vulnerable populations could be immunized vaccines to attain heterologous nonspecific protection from new coronavirus In lights our investigations most...