A5018548511- Viral-associated cancers and disorders
- Lymphoma Diagnosis and Treatment
- Cancer Genomics and Diagnostics
- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- CNS Lymphoma Diagnosis and Treatment
- RNA Research and Splicing
- Immune Cell Function and Interaction
- Prenatal Screening and Diagnostics
- Respiratory viral infections research
- Protein purification and stability
- Cystic Fibrosis Research Advances
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Circular RNAs in diseases
- Cancer Immunotherapy and Biomarkers
- Molecular Biology Techniques and Applications
- Bacteriophages and microbial interactions
- Genomics and Chromatin Dynamics
- Head and Neck Cancer Studies
- Monoclonal and Polyclonal Antibodies Research
- Lung Cancer Treatments and Mutations
- Vascular Tumors and Angiosarcomas
- CAR-T cell therapy research
- Sarcoma Diagnosis and Treatment
University of Groningen
2019-2024
University Medical Center Groningen
2019-2024
Ministry of Health
2019-2021
Institute for Medical Research
2015-2021
Nationwide Children's Hospital
2017
National University of Malaysia
2017
Universiti Putra Malaysia
2014
Abstract Background Primary central nervous system lymphoma (PCNSL) are rare mature B-cell lymphoproliferative diseases characterized by a high incidence of MYD88 L265P and CD79B Y196 hotspot mutations. Diagnosis PCNSL can be challenging. The aim the study was to analyze detection rate mutation in cell free DNA (cfDNA) plasma patients with PCNSL. Methods We analyzed digital droplet PCR (ddPCR) determine presence mutations cfDNA isolated from 24 active disease. Corresponding tumor samples...
Nasopharyngeal carcinoma (NPC) is originated from the epithelial cells of nasopharynx, Epstein–Barr virus (EBV)‐associated and has highest incidence mortality rates in Southeast Asia. Late presentation a common issue early detection could be key to reduce disease burden. Sensitivity plasma EBV DNA, an established NPC biomarker, for Stage I controversial. Most newly reported biomarkers have neither been externally validated nor compared ones. This causes difficulty planning cost‐effective...
Abstract MicroRNAs regulate gene expression at the post-transcriptional level. Differential of miRNAs can potentially be used as biomarkers for early diagnosis and prediction outcomes. Failure in validation miRNA profiles is often caused by variations experimental parameters. In this study, performance five extraction kits three RT-qPCR systems were evaluated using BioMark high-throughput platform effects different parameters on circulating levels determined. Differences well varying...
Nasopharyngeal carcinoma (NPC) is an epithelial cancer of the nasopharynx which highly associated with Epstein-Barr virus (EBV). Worldwide, most top 20 countries highest incidence and mortality rates NPC are low- middle-income countries. Many studies had demonstrated that EBV could be detected in tissue, serum plasma patients. In this study, we explored potential assays based on non-invasive nasal washings (NW) as a diagnostic prognostic tool for NPC. A total 128 patients were evaluated NW...
Nasopharyngeal carcinoma (NPC) is an epithelial squamous cell on the mucosal lining of nasopharynx. The etiology NPC remains elusive despite many reported studies. Most studies employ a single platform approach, neglecting cumulative influence both genome and transcriptome toward development. We aim to integrated pathway approach identify dysregulated pathways linked NPC. Our combines imputation GWAS data from Malaysian cohort as well published expression GSE12452 non-NPC nasopharynx...
Several human leukocyte antigen (HLA) alleles are strongly associated with susceptibility to classic Hodgkin lymphoma (cHL), also in subgroups stratified for presence of the Epstein–Barr virus (EBV). We tested hypothesis that pressure on cHL tumour cells lose HLA expression is alleles. A meta-analysis was carried out identify consistent protective and risk a combined cohort 839 patients from Netherlands United Kingdom. Tumour cell studied 338 cases these two cohorts correlated specific...
A modified phage-enzyme link immunosorbent assay (phage-ELISA) and a phage dot-blot specific for hepatitis B surface antigen (HBsAg) its antibody were developed by using display technology.The phage-ELISA assays enabled to detect HBsAg anti-HBsAg in human sera, compatible commercial detection kit.The fusion phages immobilized onto microtiter plate wells nitrocellulose membrane sheets, then blocked with 10% milk diluent, added serum at dilution of 1:5000.The absorbance 405 nm was determined...
Background: Bronchoalveolar lavage (BAL) is a widely used method in the evaluation of lung pathology adults and children. The main indications children include confirming suspected infection/inflammation, aspiration pneumonia, periodic lungs cystic fibrosis patients. Materials methods: We reviewed total 308 consecutive reports from pediatric BAL specimens as part quality assurance project aiming to compare morphologic findings cytological examination with microbiology testing evaluate impact...
Introduction Treatment failure or relapse occurs in approximately 10% of early stage Hodgkin lymphoma (HL) patients and 20% who have advanced HL. Identifying this group refractory relapsed HL is crucial order to plan for effective first line treatment. Considering that genomic aberrations can be readily detected cfDNA, method may allow a non-invasive monitoring disease load serve as prognostic marker. We aimed study the profile correlate these findings levels circulating biomarker TARC other...
EGFR-mutated non-small cell lung cancer (NSCLC) patients can be effectively treated with tyrosine kinase inhibitors (TKI) but frequently present an EGFR T790M resistance mutation at relapse. We aimed to screen for in pre-treatment formalin-fixed and paraffin-embedded (FFPE) tissue samples of a confirmed progression. analyzed 33 DNA NSCLC who progressed upon TKI between 2013 2019. To establish storage-time dependent formalin fixation-induced background levels C>T mutations, we isolated from...