A5053889384- Blood groups and transfusion
- Erythrocyte Function and Pathophysiology
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Fluorine in Organic Chemistry
- Platelet Disorders and Treatments
- Blood Coagulation and Thrombosis Mechanisms
- Synthesis and Reactions of Organic Compounds
- Synthesis and Biological Evaluation
- Glycosylation and Glycoproteins Research
- Monoclonal and Polyclonal Antibodies Research
- Hemoglobinopathies and Related Disorders
- CRISPR and Genetic Engineering
- Diabetes and associated disorders
- Synthesis of heterocyclic compounds
- Blood disorders and treatments
- Pluripotent Stem Cells Research
- Chronic Lymphocytic Leukemia Research
- Synthesis and Reactivity of Sulfur-Containing Compounds
- Complement system in diseases
- Hemostasis and retained surgical items
- Immunodeficiency and Autoimmune Disorders
- Analytical Chemistry and Chromatography
- Circular RNAs in diseases
Ruijin Hospital
2015-2025
Shanghai Jiao Tong University
2015-2025
Sichuan Cancer Hospital
2010-2020
University of Electronic Science and Technology of China
2020
Hubei University of Chinese Medicine
2018-2020
Stanford University
2014-2017
Baxter (United States)
2014-2017
Suzhou Vocational Health College
2016
Shanghai Blood Center
2008-2015
Chengdu Second People's Hospital
2015
Extracellular vesicles (EVs) contain a rich cargo of different RNA species with specialized functions and clinical applications. However, the landscape characteristics extracellular vesicle long (exLR) in human blood remain largely unknown.We presented an optimized strategy for exLR sequencing (exLR-seq) plasma. The sample cohort included 159 healthy individuals, 150 patients cancer (5 types), 43 other diseases. Bioinformatics approaches were used to analyze distribution features exLRs....
The prokaryotic type II CRISPR/Cas9 system has been adapted to perform targeted genome editing in cells and model organisms. Here, we describe gene deletion replacement human via the using two guide RNAs. effectively generated deletions of varied length, regardless transcriptional status target gene. It is notable that RNAs resulted formation correct junctions at high efficiency. Moreover, presence a homology repair donor, could precise replacement. Our results illustrate can be used...
Background Identifying genetic variants of the ABO gene may reveal new biologic mechanisms underlying variant phenotypes blood group. We report molecular analysis 322 apparently unrelated subgroup individuals in an estimated 2.1 million donors. Study Design and Methods performed phenotype investigations by serology studies, analyzed DNA sequence direct sequencing or after cloning, evaluated promoter activity reporter assays. Results In 62 rare alleles, we identified 29 novel alleles 43 their...
Background Drug-induced immune hemolytic anemia (DIIHA) is a rare but serious condition, with an estimated incidence of one in 100,000 cases, associated various antibiotics. This study reports on case ceftizoxime-induced hemolysis observed patient China. Case description A Chinese diagnosed malignant rectal cancer underwent antimicrobial therapy after laparoscopic partial recto-sigmoid resection (L-Dixon). After receiving four doses ceftizoxime, the developed symptoms including rash, itchy...
ABSTRACT The HLA‐C*01:250 allele differs from HLA‐C*01:02:01:01 by a single non‐synonymous nucleotide change in exon 7.
ABSTRACT The HLA‐A*02:1098 allele differs from HLA‐A*02:07:01:01 by a single non‐synonymous nucleotide change in exon 3.
ABSTRACT The HLA‐B*40:01:82 allele differs from HLA‐B:40:01:01 by a single synonymous nucleotide in exon 4.
Platelets are anucleated blood cells and contain various RNA species. We investigated the changes in whole transcriptome expression profile of platelet concentrates (PC) during storage to explore biological functions biomarkers damage.Platelets were collected by apheresis from eight healthy donors stored day 0 4. Platelet phenotyping function analysis used detect activity storage. RNA-sequencing was mRNA, lncRNA circRNA PC Gene ontology KEGG analyses applied predict functional distribution...
HLA‐B*48:01:13 differs from HLA‐B*48:01:01:01 by one nucleotide in exon 5.
B(x) is a very rare ABO blood group phenotype and the molecular mechanism underlying it still remains largely unknown. This study reports two novel alleles in Chinese individuals.Serologic investigations including serum transferase activity assay were performed with standard methods. DNA sequences of all seven exons exon-intron boundaries gene analyzed using genomic by polymerase chain reaction direct sequencing or after cloning.B(x) phenotypes diagnosed these individuals. analysis revealed...
A weak ABO subgroup is one of the most important causes an blood grouping discrepancy. Here, we investigated distribution subgroups in Chinese population and identified ten novel alleles.We performed phenotype investigations by serological studies, analysed DNA sequence gene direct sequencing or after cloning, evaluated role glycosyltransferase mutations silico analysis vitro expression assay.Three hundred fifty-one individuals with a were detected among 1.45 million blood-typed subjects....
Protein S (PS) deficiency is associated with a 10-fold increased risk of venous thromboembolism (VTE), but its diagnosis quite difficult and complicated. In this study, we identified 53 unrelated pedigrees PS in China. Data their clinical characteristics laboratory examinations were collected. Genetic analysis PROS1 including direct sequencing, copy number variant detection messenger ribonucleic acid was performed probands related family members. Of these probands, 52.8% (28/53) experienced...
The short life span of platelets is a major challenge to platelet transfusion services because the lack effective intervention. Here, we found that accumulation long-chain acylcarnitines (LCACs) responsible for mitochondrial damage and storage lesion. Further studies showed blockade fatty acid oxidation activation AMP-activated protein kinase (AMPK)/acetyl-CoA carboxylase/carnitine palmitoyltransferase 1 (CPT1) pathways promote metabolism are important reasons LCACs. excessive LCACs can...
BACKGROUND The amino acid substitutions caused by ABO gene mutations are usually predicted to impact glycosyltransferase's function or its biosynthesis. Here we report an exonic missense mutation that affects B‐antigen expression decreasing the mRNA level of rather than change. STUDY DESIGN AND METHODS Serologic studies including plasma total GTB transfer capacity were performed. exon sequences analyzed Sanger sequencing. B 310 cDNA with c.28G>A (p.G10R) was expressed in HeLa cells and...
The sequential reaction of 2 equiv difluorocarbene (generated from trimethylsilyl fluorosulfonyldifluoroacetate (TFDA) by treatment with catalytic fluoride ion) a series electron-rich aromatic ketones and alpha,beta-unsaturated leads to the formation difluoromethyl 2,2-difluorocyclopropyl ethers in good yield.
Resin-bound amines 1a-e condense with isothiocyanates to give thiourea resins 2a-i. Resins 2a-g subsequently react iodomethane followed by cleavage affording S-methyl isothioureas 4a-g, and 2a-b,h-i acyl chlorides afford N-acylated thioureas 6a-d. N-Acylthioureas 8a-f (R(2) = H) were prepared directly from resin-bound 1a-d isothiocyanates. 8a-d,f(R(2) used for the preparation of S-methyl-N-acylisothioureas 10a-e. Alkylation was performed using methyl iodide. 10a,b,d are converted an action...
(Benzotriazol-1-yl)carboximidamides were applied for the preparation of polysubstituted acylguanidines and guanylureas. The reaction sequence utilized mild conditions gave high yields final compounds intermediates. protocol developed allows variation substituents at all positions products.