A5101778663- Breast Cancer Treatment Studies
- Breast Implant and Reconstruction
- Cancer-related molecular mechanisms research
- Circular RNAs in diseases
- Extracellular vesicles in disease
- Reconstructive Surgery and Microvascular Techniques
- Breast Lesions and Carcinomas
- MicroRNA in disease regulation
- HER2/EGFR in Cancer Research
- Epigenetics and DNA Methylation
- Cancer Risks and Factors
- Cancer-related gene regulation
- RNA Research and Splicing
- Angiogenesis and VEGF in Cancer
- RNA regulation and disease
- Cancer Research and Treatments
- Software Engineering Research
- RNA modifications and cancer
- Reconstructive Facial Surgery Techniques
- Wnt/β-catenin signaling in development and cancer
- Ferroptosis and cancer prognosis
- Global Cancer Incidence and Screening
- Autophagy in Disease and Therapy
- Signaling Pathways in Disease
- Metastasis and carcinoma case studies
First Affiliated Hospital of Xiamen University
2025
Fudan University Shanghai Cancer Center
2018-2023
Shanghai Medical College of Fudan University
2019-2022
Fifth Affiliated Hospital of Sun Yat-sen University
2019
Sun Yat-sen University
2019
Breast Center
2018
University of Tennessee Health Science Center
2018
Center for Cancer Research
2018
Shanghai Cancer Institute
2018
Shanghai University of Traditional Chinese Medicine
2018
Extracellular vesicles (EVs) contain a rich cargo of different RNA species with specialized functions and clinical applications. However, the landscape characteristics extracellular vesicle long (exLR) in human blood remain largely unknown.We presented an optimized strategy for exLR sequencing (exLR-seq) plasma. The sample cohort included 159 healthy individuals, 150 patients cancer (5 types), 43 other diseases. Bioinformatics approaches were used to analyze distribution features exLRs....
Detection of breast cancer at an early stage is the key for successful treatment and improvement outcome. However limitations mammography are well recognized, especially those women with premenopausal cancer. Novel approaches to screening necessary, in developing world where not feasible. In this study, we examined promoter methylation six genes (SFN, P16, hMLH1, HOXD13, PCDHGB7 RASSF1a) circulating free DNA (cfDNA) extracted from serum. We used a high-throughput assay (MethyLight) examine...
Background: Chemoresistance has become a major obstacle for cancer therapy in clinic. Long noncoding RNAs (lncRNAs) have been reported to play critical roles the development of chemoresistance various tumors, including gastric (GC). However, role HOXA transcript at distal tip (HOTTIP) within extracellular vesicles (exosomes) cisplatin-resistant GC cells remains largely unknown. Materials and methods: Cell proliferation, migration invasion were detected using Counting Kit-8 (CCK-8) transwell...
Abstract A large number RNAs are enriched and stable in extracellular vesicles (EVs), they can reflect their tissue origins suitable as liquid biopsy markers for cancer diagnosis treatment efficacy prediction. In this study, we used vesicle long RNA (exLR) sequencing to characterize the plasma-derived exLRs from 112 breast patients, 19 benign patients 41 healthy participants. The different profiling was found between non-cancer groups. Thus, constructed a diagnostic signature which showed...
Abstract Background Metastasis is the predominant cause of mortality in patients with breast cancer. Long noncoding RNAs (lncRNAs) have been shown to drive important phenotypes tumors, including invasion and metastasis. However, lncRNAs involved metastasis their molecular cellular mechanisms are still largely unknown. Methods The transcriptional posttranscriptional processing LINC00478-associated cytoplasmic RNA (LacRNA) was determined by RT-qPCR, semiquantitative PCR 5′/3′ RACE....
Macrophage-capping protein (CAPG) has been shown to promote cancer cell metastasis, although the mechanism remains poorly understood.Methods: Breast (BC) tissue microarray was used test role of CAPG in prognosis BC patients.Xenograft mice model validate metastasis promotion vivo.Gene expression array, chromatin immunoprecipitation and luciferase report assay were performed search for target genes CAPG.Protein immunoprecipitation, MS/MS analysis, histone methyltransferase explore regulating...
Breast cancer in young females was usually considered more aggressive and requires therapy. We investigated whether early detection improved treatments changed the patterns of characteristics, management outcomes breast patients over time.Females under 40 years age diagnosed with during periods 1999-2017 1999-2015 were identified Fudan University Shanghai Cancer Center (FUSCC) population-based Surveillance, Epidemiology, End Results (SEER) registry, respectively. Clinicopathologic...
Abstract Background The benefit of repeat lumpectomy for ipsilateral breast tumor recurrence (IBTR) after conserving surgery is currently inconclusive. Materials and Methods Patients with IBTR definitive were identified in the Surveillance, Epidemiology, End Results registry between 1973 2013. effect different surgeries on overall cancer-specific mortality was assessed using risk-adjusted Cox proportional hazard regression modeling stratified propensity score-matching analysis (PSMA). Of...
Background The objective of this study was to determine an axillary pathologic complete response (pCR) and its influencing factors in patients with hormone receptor (HR)–positive breast cancer cytologically proven lymph node metastases. A prediction nomogram established provide information for the de‐escalation management these after neoadjuvant chemotherapy. Methods authors retrospectively enrolled all HR‐positive chemotherapy data set Fudan University Shanghai Cancer Center. All were...
Breast cancer is one of the leading causes cancer-related death among women, and pathological status axillary lymph nodes an important predictor prognosis. However, mechanism involved in this early stage metastasis remains largely unknown.Microarray analysis was used to carry out differential genomics analyses between matched pairs metastatic sentinel node tissues breast primary tumors. The CRISPR/Cas9 gene editing system for vivo screening by transplanting a loss-of-function cell pool into...
Long non-coding RNAs play an important role in breast cancer. Even with adjuvant hormone therapy, patients estrogen receptor positive cancer can present recurrences and distant metastases. We investigated whether the expression of a novel long RNA LINC00309 predict outcome cancer, especially for hormone-receptor patients.This retrospective study collected 290 including 161 hormone-positive. qPCR was performed to detect LINC00309. Kaplan-Meier Cox risk proportion model were applied disclose...
Objective To investigate the expression of proliferation cell antigen ki67 in triple negative breast cancer and to analyze its relationship clinicopathological features prognosis. Methods Immunohistochemistry was used detect tissues.Associations between various characteristics as well survival status were analysed. Result ki67 significantly associated with tumor size, TNM stage, histological grade lymph node metastasis cancer(χ2=10.536, 16.824, 11.020, 7.180, P<0.05). Univariate...
Objective To study the effect of Ulinastatin on postoperative inflammatory responses in patients with gastric cancer. Methods From June 2011 to 2012, 52 underwent radical gastrectomy for cancer were randomized two groups double-blind manner: trial group(28 cases) and control group(24 cases). Patients subjected intravenous infusion 100 000 U (UTI) twice a day 0-3, those group injected same volume saline. The level interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), white blood cell...
Objective To study the different ischemic characteristics and compensatory mechanisms of acute or chronic rats hindlimb ischemia. Methods Three months SD with hyperlipemia were used to induce (n = 20) ischemia. Real-time PCR 2-△△CT Methods analyze VEGF HIF-1α in adductor femoris tissue at 6h after femoral artery (FA) occlusion both groups. Muscle necrosis inflammation gastrocnemius observed 1 week FA occlusion. Four weeks occlusion, ratio capillary fibromuscular adductorfemoris...
In the development of software reverse engineering, program understanding is one most important steps. With brief introductions several slicing technologies and their advantages disadvantages, a new standard, used to choose criterion, proposed in this paper. By comparison with other methods, experiment shows that which calculated by standard could help programmer improve efficiency, shorten cycle reduce complexity aspect understanding.
Objective To investigate the current status of breast reconstruction surgery in China and analyze specific views Chinese doctors on relationship between radiotherapy surgery. Methods A total 110 medical institutions nationwide with more than 200 cases cancer yearly were selected into this questionnaire survey. The survey included basic information surgeons their hospitals, surgeries 2017, types surgery. Results In total, hospitals participated survey, 96(87.3%) had undergone...
Objective To evaluate the influence factors on surgical methods in DCIS (Ductal carcinoma situ) patients, and prognosis of different a 10-year single-center retrospective study. Methods We retrospectively included 1557 patients who received treatments our center from Jan. 2006 to Nov. 2016. T tests, Chi-square analysis logistic regression were used analyze methods. Kaplan-Meier Log-rank recurrence-free survival (RFS) loco-regional (LRRFS) with methods. Results Of enrolled...
Abstract Background: The utility of extracellular vesicle long RNAs (exLRs) as noninvasive biomarkers in breast cancer remains elusive. purpose this study was to explore the potential exLRs clinically actionable for diagnosis, classification, and neoadjuvant therapy efficacy prediction. Methods: One hundred seventy-two participants, including 112 patients, 19 benign patients 41 healthy controls, were enrolled case-control study. exLR profile plasma samples analyzed by sequencing. d-signature...