A5100318736- Cholesterol and Lipid Metabolism
- Drug Transport and Resistance Mechanisms
- Liver Disease Diagnosis and Treatment
- Drug-Induced Hepatotoxicity and Protection
- Peroxisome Proliferator-Activated Receptors
- Epigenetics and DNA Methylation
- Circadian rhythm and melatonin
- RNA modifications and cancer
- Cancer, Lipids, and Metabolism
- Cancer-related gene regulation
- Helicobacter pylori-related gastroenterology studies
- Genetics, Aging, and Longevity in Model Organisms
- Pharmacogenetics and Drug Metabolism
- Lipid metabolism and biosynthesis
- Endoplasmic Reticulum Stress and Disease
- MicroRNA in disease regulation
- Mast cells and histamine
- Pharmacy and Medical Practices
- Cancer Genomics and Diagnostics
- Adipose Tissue and Metabolism
- Pancreatic function and diabetes
- Lipoproteins and Cardiovascular Health
- Atherosclerosis and Cardiovascular Diseases
- Pharmacological Effects of Natural Compounds
- Adipokines, Inflammation, and Metabolic Diseases
Dalian Medical University
2025
Central South University
2025
Fudan University
2008-2024
Yangzhou University
2023
Shanghai East Hospital
2023
State Key Laboratory of Medical Neurobiology
2020-2022
Shanghai Jiao Tong University
2021-2022
Renji Hospital
2009-2022
Nanjing Medical University
2022
Qujing Normal University
2022
Detection of breast cancer at an early stage is the key for successful treatment and improvement outcome. However limitations mammography are well recognized, especially those women with premenopausal cancer. Novel approaches to screening necessary, in developing world where not feasible. In this study, we examined promoter methylation six genes (SFN, P16, hMLH1, HOXD13, PCDHGB7 RASSF1a) circulating free DNA (cfDNA) extracted from serum. We used a high-throughput assay (MethyLight) examine...
The concept of spatially fractionated radiation therapy (SFRT) was conceived over 100 years ago, first in the form GRID, which has been applied to clinical practice since its early inception and continued present even with markedly improved instrumentation therapy. LATTICE (LRT) introduced 2010 as a conceptual 3D extension GRID several uniquely different features. Since 2014, when patient treated, 150 patients bulky tumors worldwide have received LRT. Through brief review basic principles...
Abstract Cholesterol sulfate, produced by hydroxysteroid sulfotransferase 2B1 (SULT2B1), is highly abundant in the intestine. Herein, we study functional role and underlying intestinal epithelial repair mechanisms of cholesterol sulfate ulcerative colitis. The levels as well expression Sult2b1 genes involved biosynthesis, are significantly higher inflamed tissues from patients with colitis than mucosa healthy controls. gut circulation mainly catalyzed SULT2B1. Specific deletion gene cells...
Abstract Circadian genes control most of the physiological functions including cell cycle. Cell proliferation is a critical factor in differentiation progenitor cells. However, role Clock gene regulation cycle via wingless‐type (Wnt) pathway and relationship between adipogenesis are unclear. We found that circadian locomotor output cycles kaput ( ) regulated 3T3‐L1 preadipocytes. attenuation inhibited viability preadipocytes counting kit 8. The expression c‐Myc Cyclin D1 decreased...
To evaluate the potential of clinical-based model, a biparametric MRI-based radiomics model and clinical-radiomics combined for predicting clinically significant prostate cancer (PCa).In total, 381 patients with suspicious PCa were included in this retrospective study; those, 199 did not have upon biopsy, while 182 had PCa. All underwent 3.0-T MRI examinations same acquisition parameters, clinical risk factors associated (age, volume, serum PSA, etc.) collected. We randomly stratified...
The oxysterol receptor LXR is a key transcriptional regulator of lipid metabolism. increases expression SREBP-1, which in turn regulates at least 32 genes involved synthesis and transport. We recently identified 25-hydroxycholesterol-3-sulfate (25HC3S) as an important regulatory molecule the liver. have now studied effects 25HC3S its precursor, 25-hydroxycholesterol (25HC), on metabolism mediated by LXR/SREBP-1 signaling macrophages. Addition to human THP-1-derived macrophages markedly...
Due to the wide application of engineered aluminum oxide nanoparticles and increased containing particulate matter suspending in air, exposure human nano-scale (Al2O3 NPs) is becoming inevitable.In present study, RNA microarray coupled with metabolomics analysis were used uncover mechanisms underlying cellular responses Al2O3 NPs imply potential rescue.We found that significantly triggered down-regulation mitochondria-related genes located complex I, IV V, which involved oxidative...
Pancreatitis is the leading cause of hospitalization in gastroenterology, and no medications are available for treating this disease current clinical practice. FXR plays an anti-inflammatory role diverse inflammatory diseases, while its function pancreatitis remains unknown. In study, we initially observed a marked increase nuclear pancreatic tissues human patients with pancreatitis. Deleting acinar cells (FXR Δ / ) led to more severe mouse models caerulein-induced acute chronic...
MTHFD2 is highly overexpressed in breast cancer tissues, indicating that it might be used as a target treatment. This study aims to determine the role of cell proliferation and molecular pathways involved. In order investigate gene expression its downstream cancer, we started our inquiry with bioinformatics analysis. We then engineered lines either silenced or effects on cycle, proliferation, m6A methylation status IFRD1, predicted target. Overexpression enhanced cellular increased...
Background and purpose Approximately 25% of acute large vessel occlusive (LVO) ischaemic strokes are unknown thrombotic origin, there is a need to establish the aetiology guide subsequent preventative measures. The aim this study was quantify thrombus composition in patients with LVO explore associations between stroke aetiology. Methods Thrombi were extracted from 132 stroke. Erythrocytes, leucocytes F+P (fibrin+platelet) proportions assessed tissue sections stained H&E, while CD3 + T...
Abstract Pancreatic β cell loss by cellular stress contributes to diabetes pathogenesis. Nevertheless, the fundamental mechanism of regulation remains elusive. Here, it is found that elevated zinc transportation causes excessive in pancreatic cells diabetes. With gene‐edited human embryonic stem cell‐derived (SC‐β cells) and primary islets, results reveal initiates integrated response (ISR), ultimately leads death. By contrary, genetic abolishment shields from exacerbated endoplasmic...
Cellular cholesterol homeostasis is maintained through coordinated regulation of synthesis, degradation, and secretion. Nuclear receptors for oxygenated derivatives (oxysterols) are known to play key roles in the homeostasis. We recently identified a sulfated oxysterol, 5-cholesten-3beta,25-diol 3-sulfate (25HC3S), that localized liver nuclei. The present study reports biosynthetic pathway 25HC3S hepatocytes. Assays using mitochondria isolated from rats sterol 27-hydroxylase (Cyp27A1) gene...
Abstract Many daily activities are under the control of circadian clock, including nutrition metabolism and energy generation. Mitochondria, as core factories oxidizing substrates producing ATP, undergo changes in quantity morphology to adapt demand for energy. It has been demonstrated that mitochondrial gene expression, dynamics functions all affected by clock. Here, we Clock affects number, architecture function mitochondria via posttranscriptional regulation Drp1. Δ19 leads fragmented...
Background. The risk of atherosclerosis is elevated in abnormal lipid metabolism and circadian rhythm disorder. We investigated whether lighting condition would have influenced the expression clock genes clock-controlled metabolism-related ApoE-KO mice. Methods. A mouse model with disorder was established using mice (ApoE-KO LD/DL mice) by altering exposure to light. C57 BL/6J (C57 exposed normal day night diet served as control According zeitgeber time samples were acquired, test...
It is generally accepted that exposure to particulate matter (PM) increases the risk of cardiovascular-related morbidity and mortality, though exact mechanism behind this has yet be elucidated. Oxidative stress plays a potentially important role in toxicity, with Nrf2 serving as major antioxidant gene. In current study, knockout mouse model was used combination an individual ventilated cage (IVC)-based real-ambient PM system assess potential cardiotoxicity induced by related signaling...
Inhaled nanoparticles can deposit in the deep lung where they interact with pulmonary cells. Despite numerous studies on nanotoxicity, detailed molecular mechanisms of specific nanomaterial-induced injury have yet to be identified. Using whole-body dynamic inhalation model, we studied interactions between aluminum oxide (Al2O3 NPs) and system vivo. We found that seven-day-exposure Al2O3 NPs resulted emphysema small airway remodeling murine lungs, accompanied by enhanced inflammation...
Acetaminophen (APAP) overdose is one of the most common causes acute liver failure in many countries. The aim study was to describe profiling phosphatidylcholine (PC) and phosphatidylethanolamine (PE) plasma -induced injured mice. A time course carried out using C57BL/6 mice after intraperitoneal administration 300 mg/kg 1 h, 3 6 12 h 24 h. high-throughput liquid chromatography mass spectrometry (LC-MS) lipidomic method utilized detect species liver. expressions metabolism related genes were...