A5101811286- Bacterial Genetics and Biotechnology
- Bacteriophages and microbial interactions
- Enzyme Production and Characterization
- Microbial Metabolic Engineering and Bioproduction
- Biofuel production and bioconversion
- RNA and protein synthesis mechanisms
- Pancreatic function and diabetes
- Hormonal Regulation and Hypertension
- Protein Hydrolysis and Bioactive Peptides
- Diet, Metabolism, and Disease
- Adipose Tissue and Metabolism
- Financial Distress and Bankruptcy Prediction
- Bacterial biofilms and quorum sensing
- Cancer Research and Treatments
- Studies on Chitinases and Chitosanases
- Enzyme Structure and Function
- Viral Infectious Diseases and Gene Expression in Insects
- Enzyme Catalysis and Immobilization
- Apelin-related biomedical research
- Viral Infections and Vectors
- Connexins and lens biology
- Cassava research and cyanide
- Escherichia coli research studies
- Vitamin K Research Studies
- Plant-derived Lignans Synthesis and Bioactivity
Beijing Haidian Hospital
2024
Tianjin Institute of Industrial Biotechnology
2014-2024
Chinese Academy of Sciences
2014-2024
Shenzhen Third People’s Hospital
2024
University of Chinese Academy of Sciences
2024
Hunan University of Traditional Chinese Medicine
2024
Sichuan Agricultural University
2012-2019
First Bethune Hospital of Jilin University
2012
First Hospital of Jilin University
2012
Jilin University
2012
Leptospirosis is a widely spread disease of global concern. Infection causes flu-like episodes with frequent severe renal and hepatic damage, such as haemorrhage jaundice. In more cases, massive pulmonary haemorrhages, including fatal sudden haemoptysis, can occur1. Here we report the complete genomic sequence representative virulent serovar type strain (Lai)2 Leptospira interrogans serogroup Icterohaemorrhagiae consisting 4.33-megabase large chromosome 359-kilobase small chromosome, total...
The use of Bacillus subtilis in synthetic biology and metabolic engineering is highly desirable to take advantage the unique pathways present this organism. To do this, an evaluation B. subtilis' intrinsic biological parts required determine best strategies accurately regulate circuits expression target proteins. strengths promoter candidates were evaluated by measuring relative fluorescence units a green fluorescent protein reporter, integrated into chromosome. A total 84 predicted...
SignificanceA gene regulatory system is an important tool for the engineering of biosynthetic pathways organisms. Here, we report development inducible-ON/OFF using a malO operator as key element. We identified and modulated sequence, position, numbers, spacing distance operators, generating series activating or repressive promoters with tunable strength. The stringency robustness are both guaranteed in this system, maximal induction factor 790-fold was achieved, nine proteins from different...
Secretory expression of valuable proteins by B. subtilis and its related species has attracted intensive work over the past three decades. Although very high yields can be achieved with homologous proteins, production heterologous is unfortunately not straight forward. The Sec pathway major route for protein secretion in subtilis. Therefore, aim this was to identify bottlenecks improve molecular genetic techniques. Two α-amylases (AmyL AmyS) both under control P HpaII promoter equipped their...
Bacillus subtilis is widely used for large-scale industrial production of heterologous proteins. Because its high intrinsic secretory capacity, it can efficiently secrete proteins into the culture supernatant via general Sec-type secretion pathway. In this study, α-amylase AmyS was as a reporter to construct library encompassing 173 signal peptides (SPs) from B. using fast, sequence-independent method. The resulting DNA which harbored different in expression vector transform directly at...
The Gram-positive bacterium Bacillus subtilis has been widely used as a cell factory for the production of proteins due to its generally regarded safe (GRAS) nature and secretion capability. Of known secretory pathways in B. subtilis, majority are exported from cytoplasm by Sec pathway, Tat pathway ABC transporters, etc. However, heterologous is unfortunately not that straight forward because bottlenecks classical pathways. aim this work explore new method protein based on non-classical...
The manno endo-1,4-mannosidase (β-mannanase, EC. 3.2.1.78) catalyzes the random hydrolysis of internal (1 → 4)-β-mannosidic linkages in mannan polymers. A codon optimized β-mannanase gene from Bacillus licheniformis DSM13 was expressed subtilis. When four Sec-dependent and two Tat-dependent signal peptide sequences cloned B. subtilis were placed upstream target gene, highest activity observed using SPlipA as a peptide. Then 1.25-fold obtained when another copy groESL operon inserted into...
Many advances in modifying enzyme properties have been made based on the computation of stable transition states and experimental directed evolution. However, there are still great challenges related to difficult state (TS) calculations large number evolutionary experiments needed for verification. The analogue (TSA) has chemical similarity with TS structure can be used as a proxy unknown TS. Here, we proposed computation-driven strategy optimize structures enzymes via simulation-based...
Recombinant human Fibroblast growth factor 21 (rhFGF21) is an endocrine hormone that has profound effects on treatment of metabolic diseases. However, rhFGF21 prone to form inclusion body when expressed in bacteria, which results in, the downstream process purification bioactive time-consuming and labor intensive. The aim this work explore a new method for improving soluble expression secretion level B. subtilis. A codon optimized gene was under control strong inducible promoter PmalA...
Limitations of enzyme production and activity pose a challenge for efficient degradation chitinaceous wastes. To solve this problem, we engineered system high-yielding extracellular secretion chitinase A1 from Bacillus circulans (BcChiA1) in B. subtilis. Furthermore, an innovative high-throughput screening method based on colloidal chitin stained with Remazol Brilliant Blue R (CC-RBB) was established used to identify three mutants improved activity: Y10A/R301A/E327A (Mu1), Y10A/D81A/E327A...
Abstract d ‐Psicose 3‐epimerase is an enzyme that catalyzes the synthesis of ‐psicose from ‐fructose. We cloned Ruminococcus sp. (RDPE) and expressed it in Bacillus subtilis A311. By a two‐step pH regulation segmented fermentation, we significantly improved RDPE production decreased fermentation cost. The consisted first step maintained value at 7.0 for 24 H second adjusted up to 7.5 slowly another H. Finally, was increased 74 U/mL, which about 2.5‐fold compared with control. Our strategy...
The 29 plant species in the Kadsura genus of Schisandraceae family are mainly distributed eastern and southeas-tern Asia. Ten plants this China, some which folk medicinal with activating blood circulation, relieving pain, dispelling wind, dehumidifying effects. Their main constituents lignans triterpenes. current pharmacology clinical studies have shown that their extracts anti-rheumatoid arthritis, liver protection, antioxidation, anti-inflammatory, other biological activities....
Wang, Liping, Gang Fu, Ruijuan Han, Peijia Fan, Jing Yang, Kerui Gong, Zhijun Zhao, Chunyang Zhang, Kai Sun, and Guo Shao. MALAT1 NEAT1 are neuroprotective during hypoxic preconditioning in the mouse hippocampus possibly by regulation of NR2B.
The signal recognition particle (SRP) is conserved in all living organisms, and it cotranslationally delivers proteins to the inner membrane or endoplasmic reticulum. Recently, SRP loss was found not be lethal either eukaryote Saccharomyces cerevisiae prokaryote Streptococcus mutans In Escherichia coli, role of mediating protein (IMP) targeting has long been studied. However, essentiality remains a controversial topic, partly hindered by lack strains which completely absent. Here we show...