A5004413763- Atrial Fibrillation Management and Outcomes
- Cardiac Arrhythmias and Treatments
- Mesenchymal stem cell research
- Tissue Engineering and Regenerative Medicine
- Circular RNAs in diseases
- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- Cardiac pacing and defibrillation studies
- Cardiac Imaging and Diagnostics
- Congenital heart defects research
- Fatigue and fracture mechanics
- Autophagy in Disease and Therapy
- Congenital Anomalies and Fetal Surgery
- Cardiac electrophysiology and arrhythmias
- Aortic aneurysm repair treatments
- Electrospun Nanofibers in Biomedical Applications
- Extracellular vesicles in disease
- Aortic Disease and Treatment Approaches
- Transplantation: Methods and Outcomes
- RNA modifications and cancer
- Acute Kidney Injury Research
- Congenital Diaphragmatic Hernia Studies
- Antiplatelet Therapy and Cardiovascular Diseases
- Cancer, Hypoxia, and Metabolism
- Cardiovascular and Diving-Related Complications
Taizhou People's Hospital
2015-2024
Nantong University
2015-2024
Nanjing Medical University
2023-2024
Zhongda Hospital Southeast University
2021-2023
Contrast-induced acute kidney injury (CI-AKI) causes clinically acquired nephropathy in patients who undergo coronary interventions. Hypoxic to proximal tubular epithelial cells is a pathological mechanism of CI-AKI. Previous studies have shown that hypoxia activates HIF-1α/HE4/NF-κB enhance renal fibrosis, and the SGLT-2 inhibitor luseogliflozin inhibits hypoxia-inducible factor (HIF)-1α expression reduce progression diabetic nephropathy. However, therapeutic effects mechanisms inhibitors...
Human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) are in the foreground as a preferable application for treating diseases. However, safety of hUC-MSCs after long-term culturing vitro serum-free medium remains unclear.hUC-MSCs were separated by adherent tissue culture. cultured MesenCult-XF and FBS-bases DMEM complete medium. At 1st, 3rd, 5th, 8th, 10th, 15th passage, differentiation MSCs into osteogenic, chondrogenic, adipogenic was detected, MTT, surface antigens measured....
Purpose. Cryoprotectants (CPA) for stem cells from umbilical cord blood (UCB) have been widely developed based on empirical evidence, but there is no consensus a standard protocol of preservation the UCB cells. Methods. In this study, 115 donors was collected. Each unit divided into four equal parts and frozen in different kinds cryoprotectant as follows: group A, 10% ethylene glycol 2.0% dimethyl sulfoxide (DMSO) (v/v); B, DMSO dextran-40; C, 2.5% (v/v) + 30 mmol/L trehalose; D, without...
Abstract Introduction Although mesenchymal stem cells (MSCs) from different sources share many similar characteristics, they also exhibit individual properties. In this study, we compared MSCs derived Wharton’s jelly in the umbilical cord with those decidual basalis maternal part of placenta to better understand similarities and differences between these two cell types. Method The morphology, immunophenotype (as assessed using flow cytometry), multi-lineage differentiation potential were...
The specific pathogenesis of atrial fibrillation (AF) remains unclear. In this study, we examined the expression differential messenger RNAs (mRNAs), circular (circRNAs), and long-stranded noncoding (lncRNAs) from human peripheral blood mononuclear cells to initially construct a circRNA/lncRNA-miRNA-mRNA ceRNA regulatory network explore AF screen for potential biomarkers.A total four pairs cases healthy subjects were selected detect differentially expressed mRNAs, circRNAs, lncRNAs in by...
So far, there were no reports on circular RNA (circRNA) expression profiles in the differentiation of human umbilical cord-derived mesenchymal stem cells (hUCMSCs) into cardiomyocyte-like induced by 5-aza. In this study, hUCMSCs isolated from cords and with 5-aza for 14 days. Immunofluorescence staining, real-time reverse transcription polymerase chain reaction (RT-PCR), western blot cardiac troponin I α-sarcomeric actin between Days 0 performed. The profile circRNAs was analyzed microarray...
To investigate the expression profiles of long noncoding RNAs (lncRNAs) in patients with atrial fibrillation (AF).The peripheral blood monocytes a total 20 AF and healthy subjects were collected for gene chip technology to detect differentially expressed lncRNAs from 2017.01 2017.08. Reverse transcription polymerase chain reaction (RT-PCR) was applied further verification. Gene Ontology (GO) Kyoto Encyclopedia Genes Genomes (KEGG) analyses performed identify functions genes related...
The risk factors associated with death in complicated Stanford B acute aortic dissection (AAD) after thoracic endovascular repair (TEVAR) are poorly understood. aim of this study was to evaluate the early and late events mortality AAD TEVAR.Sixty-two patients undergoing TEVAR were included study.Primary technical success achieved 61 (98.39%) cases. rate 9.68%. Procedural type I endoleak (p = 0.007, OR 7.71, 95% CI: 1.75-34.01) cardiac tamponade 0.010, 8.86, 1.70-4 6.14) significant...
The purpose of the study was to explore mechanism by which myocardial ischemia-reperfusion (I/R) injury-induced exosomes modulate mesenchymal stem cells (MSCs) regulate injury. In this study, we established an I/R injury model in vivo and a hypoxia-reoxygenation (H/R) vitro . Then, isolated from H/R-exposed H9c2 were characterized using transmission electron microscopy (TEM), nanoparticle tracking analysis (NTA), Western blot analysis. CCK-8 assays flow cytometry performed assess cell ELISA...
Background. Circulating galectin-3 (Gal-3) and aldosterone (ALD) are involved in fibrosis inflammation. However, their potential value as predictors of atrial fibrillation (AF) recurrence after radiofrequency catheter ablation (RFCA) is unknown or controversial. Therefore, the aim this study was to assess relationship between baseline Gal-3, ALD levels, AF patients performing RFCA. Methods. 153 consecutive undergoing RFCA were included. Gal-3 measured at baseline. Univariate multivariate Cox...
Purpose. This study sought to investigate the predictive factors for atrial fibrillation (AF) recurrence in patients after radiofrequency ablation (RFCA) and construct a nomogram prediction model providing precious information of ablative strategies. Methods. A total 221 with AF who underwent RFCA were enrolled. Univariate multivariate Cox regression used screen predictors recurrence. The receiver operating characteristic (ROC) curve Kaplan–Meier (K–M) drawn analyze value predictors. was...
The aim of this study was to investigate whether the chromosomes human umbilical cord-derived mesenchymal stem cells (hUCMSCs) change following in vitro culture for several generations. In present study, cords from two healthy infants cesarean delivery were collected aseptically and hUCMSCs isolated by digestion with collagenase trypsin, then cultured vitro. fibroblastic morphology presented cord tissue after 7 days adherent culture. When 6 passages vitro, maintained a stable spindle-shaped...
Importance The optimal strategy of combining left atrial appendage occlusion (LAAO) with catheter ablation (CA) in patients fibrillation (AF) during a single procedure remains unclear. Objective To determine the effects ablation-first vs occlusion-first strategies on long-term clinical outcomes among undergoing combined LAAO and CA procedure. Design, Setting, Participants prospective, multicenter COMBINATION randomized trial was conducted 14 high-volume centers China. Enrollment nonvalvular...
To investigate the molecular mechanism underlying differentiation of human umbilical cord-derived mesenchymal stem cells (hUCMSCs) into myocardial induced by 5-azacytidine (5-aza), and to explore expression significance DLL4-Notch signaling in this process.hUCMSCs were isolated purified from cords normal or cesarean term deliveries under sterile conditions. After treatment with 5-aza for 24 h, hUCMSCs was continued culture, GATA4 NKx2.5 at 4 weeks after induction, DLL4 Notch1 mRNA 1d, 3d,...
The aim of this study was to evaluate the effect cotransplanting mononuclear cells from cord blood (CB-MNCs) and mesenchymal stem (MSCs) as treatment for myocardial infarction (MI). Transplanting CD34+ or MSCs separately has been shown effective in treating MI, but CB-MNCs is not clear. In study, were separated by their adherence tissue culture. morphology, immunophenotype, multilineage potential analyzed. lymphocyte separation medium 1.077. cell count viability analyzed flow cytometry....
To compare the results of computed tomography angiography (CTA), transesophageal echocardiography (TEE), and digital subtraction (DSA) measurements analyze their accuracy, correlation, consistency in patients who have successfully undergone left atrial appendage closure (LAAC).A total 157 non-valvular fibrillation (AF) underwent LAAC with Watchman devices were included study. The maximum diameter depth LAA recorded using CTA, TEE, DSA. Correlations agreements compared.The procedure was...
At present, there is no effective treatment for myocardial fibrosis in atrial fibrillation (AF). It reported that miR-15a-5p abnormally expressed AF patients but its specific role remains unclear. This study aims to investigate the effect of fibrosis.Left appendage (LAA) tissues were collected from and non-AF patients. In lipopolysaccharide (LPS) stimulated H9C2 cells, mimic, inhibitor, pcDNA3.1-Smad7 small interfering RNA-Smad7 (siRNA-Smad7) respectively transfected up-regulate or...
Uncomplicated Stanford B acute aortic dissection (AAD) is generally treated with medical management; whereas complicated dissections require surgery or thoracic endovascular repair (TEVAR). Studies have demonstrated that long-term outcomes management are suboptimal. Therefore, we sought to investigate the early and clinical efficacy of TEVAR for AAD.From March 2004 January 2008, 63 consecutive patients were retrospectively placed into either one two groups, group (n = 42) medicine 21). All...