A5090979073- Ion channel regulation and function
- Cardiac electrophysiology and arrhythmias
- Nicotinic Acetylcholine Receptors Study
- Receptor Mechanisms and Signaling
- Neuroscience and Neuropharmacology Research
- Venomous Animal Envenomation and Studies
- Insect and Pesticide Research
- Neuroscience and Neural Engineering
- Cardiomyopathy and Myosin Studies
- Cardiac pacing and defibrillation studies
- Cardiac Arrhythmias and Treatments
- Healthcare and Venom Research
- Photoreceptor and optogenetics research
- Photochromic and Fluorescence Chemistry
- Beetle Biology and Toxicology Studies
- Neurogenetic and Muscular Disorders Research
- RNA and protein synthesis mechanisms
- Cardiovascular Effects of Exercise
- Muscle Physiology and Disorders
- RNA Research and Splicing
- Plant-based Medicinal Research
- Chemical Synthesis and Analysis
- Phosphodiesterase function and regulation
- Hormonal and reproductive studies
University of Bern
2024
Institut du Thorax
2017-2023
Nantes Université
2017-2023
Centre National de la Recherche Scientifique
2017-2023
Inserm
2017-2023
Laboratoire d'Excellence Canaux Ioniques d'Intérêt Thérapeutique
2019-2023
Centre Hospitalier Universitaire de Nantes
2022
Phlotoxin-1 (PhlTx1) is a peptide previously identified in tarantula venom (Phlogius species) that belongs to the inhibitory cysteine-knot (ICK) toxin family. Like many ICK-based spider toxins, synthesis of PhlTx1 appears particularly challenging, mostly for obtaining appropriate folding and concomitant suitable disulfide bridge formation. Herein, we describe procedure chemical directed sequential formation allows straightforward production this challenging peptide. We also performed...
◼ photochemotherapy potassium channels zebrafish
Abstract Sinus node (SAN) dysfunction (SND) manifests as low heart rate (HR) and is often accompanied by atrial tachycardia or atrioventricular (AV) block. The only currently available therapy for chronic SND the implantation of an electronic pacemaker. Because growing burden in population, new pharmacological therapies block are desirable. We developed a collection genetically modified mouse strains recapitulating human primary associated with different degrees AV These mice were generated...
Protoxin II (ProTx II) is a high affinity gating modifier that thought to selectively block the Nav 1.7 voltage-dependent Na+ channel, major therapeutic target for control of pain. We aimed at producing ProTx analogues entitled with novel functionalities cell distribution studies and biochemical characterization its channel targets.We took advantage properties peptide, combined slow off rate, design number new tagged useful imaging biochemistry purposes. used high-throughput automated...
Nav1.1 is an important pharmacological target as this voltage-gated sodium channel involved in neurological and cardiac syndromes. Channel activators are actively sought to try compensate for haploinsufficiency several of these pathologies. Herein we used a natural source new peptide compounds active on ion channels screened drugs capable inhibit inactivation way decreased function. We discovered that JzTx-34 highly subsequently performed full structure-activity relationship investigation...
IKr current, a major component of cardiac repolarization, is mediated by human Ether-à-go-go-Related Gene (hERG, Kv11.1) potassium channels. The blockage these channels pharmacological compounds associated to drug-induced long QT syndrome (LQTS), which life-threatening disorder characterized ventricular arrhythmias and defects in repolarization that can be illustrated using cardiomyocytes derived from human-induced pluripotent stem cells (hiPS-CMs). This study was meant assess the...
The medical staff is often powerless to treat patients affected by drug abuse or misuse and poisoning. In the case of envenomation, treatment choice remains horse sera administration that poses a wealth other conditions threats. Previously, we have demonstrated DNA-based aptamers represent powerful neutralizing tools for lethal animal toxins venomous origin. Herein, further pursued our investigations in order understand whether all toxin-interacting possessed equivalent potencies neutralize...
Abstract SMN protein deficiency causes motoneuron disease spinal muscular atrophy (SMA). SMN-based therapies improve patient motor symptoms to variable degrees. An early hallmark of SMA is the perturbation neuromuscular junction (NMJ), a synapse between and muscle cell. NMJ formation depends on acetylcholine receptor (AChR) clustering triggered by agrin its co-receptors lipoprotein receptor-related 4 (LRP4) transmembrane muscle-specific kinase (MuSK) signalling pathway. We have previously...
Voltage-gated Na+ (NaV) channels are significant therapeutic targets for the treatment of cardiac and neurological disorders, thus promoting search novel NaV channel ligands. With objective discovering new blockers ligands, we screened an In-House vegetal alkaloid library using fluorescence cell-based assays. We 62 isoquinoline alkaloids (IA) their ability to decrease FRET signal voltage sensor probes (VSP), which were induced by activation with batrachotoxin (BTX) in GH3b6 cells. This led...
Ryanodine receptors are responsible for the massive release of calcium from sarcoplasmic reticulum that triggers heart muscle contraction. Maurocalcin (MCa) is a 33 amino acid peptide toxin known to target skeletal ryanodine receptor. We investigated effect MCa and its analog MCaE12A on isolated cardiac receptor (RyR2), showed they increase RyR2 sensitivity cytoplasmic concentrations promoting channel opening decreases inhibiting concentrations. By measuring intracellular Ca2+ transients,...
Catecholaminergic Polymorphic Ventricular Tachycardia (CPVT) is an exercise and emotional stress-induced life-threatening inherited heart rhythm disorder, characterized by abnormal cellular calcium homeostasis. Most reported cases have been linked to mutations in the gene encoding type 2 ryanodine receptor gene, RYR2. We generated induced pluripotent stem cells (hiPSCs) from peripheral blood mononuclear (PBMC) three CPVT-affected patients, two of them carrying p.R4959Q mutation one p.Y2476D...
Abstract RATIONALE Cardiac rhythm, conduction and synchronization of electrical activity require the coordinated action different types ion channels that differ according to transmural regional specificities. Classical pharmacology affects these in a non-regionalized way which explains why treating arrhythmias, often occur specific foci, has limited efficacy addition negative side-effects on non-targeted organs. Photopharmacology is an emergent technology potential counteract all aspects...