A5090832877- Alzheimer's disease research and treatments
- CRISPR and Genetic Engineering
- Advanced biosensing and bioanalysis techniques
- Paraoxonase enzyme and polymorphisms
- Pesticide Exposure and Toxicity
- Chromosomal and Genetic Variations
- Vitamin C and Antioxidants Research
- Marine Toxins and Detection Methods
- RNA modifications and cancer
- Genomics and Phylogenetic Studies
- Bioactive Natural Diterpenoids Research
- Tryptophan and brain disorders
- VLSI and Analog Circuit Testing
- Diatoms and Algae Research
- Cytokine Signaling Pathways and Interactions
- Monoclonal and Polyclonal Antibodies Research
- Software Testing and Debugging Techniques
- Analytical Chemistry and Sensors
- RNA regulation and disease
- Business Process Modeling and Analysis
- Advanced Biosensing Techniques and Applications
- Inflammatory mediators and NSAID effects
- Salivary Gland Disorders and Functions
- Nicotinic Acetylcholine Receptors Study
- Bioinformatics and Genomic Networks
University Foundation
2024
Merck & Co., Inc., Rahway, NJ, USA (United States)
2018-2021
Source BioScience (United Kingdom)
2020
Imperial College Healthcare NHS Trust
2013
GenProMarkers (United States)
2012
Biomark (United States)
2012
University of Washington Medical Center
2006-2010
University of Washington
2005-2010
University Hospitals Sussex NHS Foundation Trust
2008
We developed PolyA-seq, a strand-specific and quantitative method for high-throughput sequencing of 3' ends polyadenylated transcripts, used it to globally map polyadenylation (polyA) sites in 24 matched tissues human, rhesus, dog, mouse, rat. show that PolyA-seq is as accurate existing RNA (RNA-seq) approaches digital gene expression (DGE), enabling simultaneous mapping polyA measurement their usage. In we confirmed 158,533 known discovered 280,857 novel (FDR < 2.5%). On average 10% human...
Saliva provides a useful and noninvasive alternative to blood for many biomedical diagnostic assays. The level of the hormone cortisol in saliva is related stress. We present here development portable surface plasmon resonance (SPR) biosensor system detection saliva. Cortisol-specific monoclonal antibodies were used develop competition assay with six-channel SPR designed our laboratory. limit laboratory buffers was 0.36 ng/mL (1.0 nM). An in-line filter based on diffusion through hollow...
The high-density lipoprotein-associated enzyme paraoxonase 1 (PON1) hydrolyzes lactones, aromatic esters, and neurotoxic organophosphorus (OP) compounds, including insecticide metabolites nerve agents. Experiments with mice lacking PON1 ( −/− mice) have established that plasma protects against chlorpyrifos/chlorpyrifos-oxon diazinon/diazoxon (DZO) exposure but does not protect parathion/paraoxon or catalytic efficiency of determines whether it will a given OP exposure. Expression active...
Severe forms of psoriasis can be complicated by systemic microvascular hyperpermeability. Vascular endothelial growth factor (VEGF) possesses potent vascular permeability activity. We suggest that VEGF enters the circulation and acts on microvessels to mediate hyperpermeability.To quantify renal circulating concentration in severe psoriasis, investigate relationship between plasma skin joint involvement.Inception cohort studies patients with generalized pustular plaque psoriasis.St John's...
Alzheimer's disease (AD) is a chronic neurodegenerative with pathological hallmarks including the formation of extracellular aggregates amyloid-beta (Aβ) known as plaques and intracellular tau tangles. Coincident Aβ recruitment activation glial cells to plaque forming niche. In addition histological data showing niche, AD genetic studies have added growing appreciation how dysfunctional glia pathways drive neuropathology, emphasis on microglia pathways. Genomic approaches enable comparisons...
Massively parallel sequencing technologies have made it possible to generate large quantities of sequence data. However, as research-associated information is transferred into clinical practice, cost and throughput constraints generally require sequence-specific targeted analyses. Therefore, sample enrichment methods been developed meet the needs applications. current amplification hybrid capture are limited in contiguous length sequences for which they able enrich. PCR based also loses...
Aggressive fibromatosis is a rare, benign proliferative disease with unknown aetiology and high recurrence rate. To date, there are only eight reported cases affecting the larynx. Four were managed total laryngectomy, whilst spontaneous regression happened in one case. Spindle cell carcinoma rare but highly aggressive biphasic tumour which often arises head neck. Diagnosed laryngeal more than 10 years ago, our patient had undergone six trans-oral laser debulking procedures. On this occasion,...
Abstract Background SARS-CoV-2 genotyping has been instrumental to monitor virus evolution and transmission during the pandemic. The reliability of information extracted from efforts depends on a number aspects, including quality input material, applied technology potential laboratory-specific biases. These variables must be monitored ensure genotype reliability. current lack guidelines for leads inclusion error-containing genome sequences in studies viral spread evolution. Results We used...
Background: Thoracic aortic aneurysms (TAA) and dissections are not uncommon causes of sudden death in young adults. Loeys-Dietz syndrome (LDS) is a rare, recently described, autosomal dominant, connective tissue disease characterized by aggressive arterial aneurysms, resulting from mutations the transforming growth factor beta (TGFβ) receptor genes TGFBR1 TGFBR2. Mean age at 26.1 years, most often due to dissection. We report an unusually late presentation LDS, diagnosed following elective...
The programmable sequence specificity of CRISPR has found uses in gene editing and diagnostics. This manuscript describes an additional application through a family novel DNA enrichment technologies. CAMP (CRISPR Associated Multiplexed PCR) cCAMP (chimeric utilize the Cas9/sgRNA complex to target loci for ligation universal adapter that is used subsequent amplification. cTRACE Targeting Rare Alleles with CRISPR-based Enrichment) also applies this method use addition adapters, however it...
In recent years, assurance of clonality the production cell line has been emphasized by health authorities during review regulatory submissions. When insufficient is provided, augmented control strategies may be required for a commercial process. this study, we conducted retrospective assessment legacy through analysis subclones from master bank (MCB). Twenty-four were randomly selected based on predetermined acceptance sampling plan. All these share conserved integration junction, thus...
Next-generation sequencing (NGS) is quickly replacing other methods for determining expression profiling of RNA as well single-nucleotide variations within the genomes both model organisms and human samples.At Boston facility Merck Research Laboratories, we concentrate on preparation samples where research needs cannot be met by commercial venders.These unmet may due to either availability up-to-date protocols or deadline constraints.In addition validating developing new libraryconstruction...
Individual alignments of UCE loci in nexus format. Loci were aligned using MAFFT and trimmed wtih Gblocks.