A5054380056- Renal Diseases and Glomerulopathies
- Acute Ischemic Stroke Management
- Neuroinflammation and Neurodegeneration Mechanisms
- RNA modifications and cancer
- Neurological Disease Mechanisms and Treatments
- RNA Research and Splicing
- Cerebrovascular and Carotid Artery Diseases
- Cancer-related molecular mechanisms research
- Mesenchymal stem cell research
- Hepatitis B Virus Studies
- Molecular Biology Techniques and Applications
- Intracerebral and Subarachnoid Hemorrhage Research
- Genomics and Chromatin Dynamics
- Cell Adhesion Molecules Research
- Hormonal Regulation and Hypertension
- Cytokine Signaling Pathways and Interactions
- Fibroblast Growth Factor Research
- Mitochondrial Function and Pathology
- Neurogenesis and neuroplasticity mechanisms
- Cancer Genomics and Diagnostics
- Circular RNAs in diseases
- Long-Term Effects of COVID-19
- Forensic and Genetic Research
- S100 Proteins and Annexins
- Respiratory viral infections research
Fujian Medical University
2016-2025
Union Hospital
2016-2025
The First People's Hospital of Changzhou
2017-2025
South China Agricultural University
2021-2024
Fujian Institute of Microbiology
2013-2024
Soochow University
2018-2024
Mengchao Hepatobiliary Hospital
2023-2024
Binzhou University
2024
Binzhou Medical University
2024
State Key Laboratory of Food Science and Technology
2024
We developed PolyA-seq, a strand-specific and quantitative method for high-throughput sequencing of 3' ends polyadenylated transcripts, used it to globally map polyadenylation (polyA) sites in 24 matched tissues human, rhesus, dog, mouse, rat. show that PolyA-seq is as accurate existing RNA (RNA-seq) approaches digital gene expression (DGE), enabling simultaneous mapping polyA measurement their usage. In we confirmed 158,533 known discovered 280,857 novel (FDR < 2.5%). On average 10% human...
Hepatocellular carcinoma (HCC) is one of the most deadly cancers worldwide and has no effective treatment, yet molecular basis hepatocarcinogenesis remains largely unknown. Here we report findings from a whole-genome sequencing (WGS) study 88 matched HCC tumor/normal pairs, 81 which are Hepatitis B virus (HBV) positive, seeking to identify genetically altered genes pathways implicated in HBV-associated HCC. We find beta-catenin be frequently mutated oncogene (15.9%) TP53 tumor suppressor...
The cynomolgus and Chinese rhesus macaques are used as animal models in biomedical research. Yan et al. sequence their genomes compare the sequences to that of Indian macaque, providing a genetic foundation for interpreting research results. nonhuman primates most commonly medical from genus Macaca1. To better understand differences between these models, we present high-quality draft genome two macaque species, cynomolgus/crab-eating macaque. Comparison with previously sequenced reveals all...
Ischemic stroke can induce changes in mitochondrial morphology and function. As a regulatory gene mitochondria, optic atrophy 1 (OPA1) plays pivotal role the regulation of dynamics other related functions. However, its roles cerebral ischemia-related conditions are barely understood.
Abstract Due to large specific surface area, abundant functional groups and low cost, biochar is widely used for pollutant removal. The adsorption performance of related synthesis parameters. But the influence factor numerous, traditional experimental enumeration powerless. In recent years, machine learning has been gradually employed biochar, but there no comprehensive review on whole process regulation adsorbents, covering optimization modeling. This article systematically summarized...
Housekeeping genes (HKG) are constitutively expressed in all tissues while tissue-enriched (TEG) at a much higher level single tissue type than others. HKGs serve as valuable experimental controls gene and protein expression experiments, TEGs tend to represent distinct physiological processes frequently candidates for biomarkers or drug targets. The genomic features of these two groups opposing patterns may shed light on the mechanisms by which cells maintain basic tissue-specific functions....
The incidence rate of lung adenocarcinoma (LUAD), the predominant histological subtype cancer, is elevated in Asians, particularly female nonsmokers. mutation patterns LUAD Asians might be distinct from those whites.We profiled 271 resected tumors (mainly stage I) to characterize genomic landscape with a focus on nonsmokers.Mutations EGFR, KRAS, erb-b2 receptor tyrosine kinase 2 gene (ERBB2), and BRAF; fusions involving anaplastic lymphoma (ALK), ROS1, ret proto-oncogene (RET); Met...
Non-coding RNAs (ncRNAs) are an essential class of molecular species that have been difficult to monitor on high throughput platforms due frequent lack polyadenylation. Using a polyadenylation-neutral amplification protocol and next-generation sequencing, we explore ncRNA expression in eleven human tissues. ncRNAs 7SL, U2, 7SK, HBII-52 expressed at levels far exceeding mRNAs. C/D H/ACA box snoRNAs associated with rRNA methylation pseudouridylation, respectively: spleen expresses both,...
RNA-based next-generation sequencing (RNA-Seq) provides a tremendous amount of new information regarding gene and transcript structure, expression regulation. This is particularly true for non-coding RNAs where whole transcriptome analyses have revealed that the much genome transcribed many transcripts widespread functionality. However, uniform resources raw, cleaned processed RNA-Seq data are sparse most organisms this especially non-human primates (NHPs). Here, we describe large-scale...
As a classic immunoregulatory and anti-inflammatory cytokine, interleukin-10 (IL-10) provides neuroprotection in cerebral ischemia vivo or oxygen-glucose deprivation (OGD)-induced injury vitro. However, it remains blurred whether IL-10 promotes neurite outgrowth synapse formation cultured primary cortical neurons after OGD injury. In order to evaluate its effect on neuronal apoptosis, formation, we administered neutralizing antibody (IL-10NA) rat We found that treatment activated the Janus...
Abstract Background Computational and microarray-based experimental approaches were used to generate a comprehensive transcript index for the human genome. Oligonucleotide probes designed from approximately 50,000 known predicted sequences genome survey transcription diverse set of 60 tissues cell lines using ink-jet microarrays. Further, expression activity over at least six conditions was more generally assessed genomic tiling arrays consisting tiled through repeat-masked version sequence...
Aldosterone (Aldo) stimulates glomerular mesangial cell (MC) proliferation, in part, through an ERK1/2-dependent pathway. In this study, we examined whether Aldo activation of ERK1/2 MC is mediated redox-dependent EGF receptor (EGFR) transactivation, as well the involvement other signaling mechanisms Aldo-induced proliferation. increased human determined by [(3)H]thymidine incorporation and counts. This increase proliferation was blocked inhibition mineralocorticoid (MR). Continuing our...
As a secreted axon guidance molecule, Netrin-1 has been documented to be neuroprotective factor, which can reduce infarct volume, promote angiogenesis and anti-apoptosis after stroke in rodents. However, its role axonal regeneration synaptic formation cerebral ischemic injury, the related underlying mechanisms remain blurred. In this study, we used Adeno-associated vectors carrying gene (AAV-NT-1) up-regulate expression level of rats' brain middle artery occlusion (MCAO). We found that...
Treadmill exercise and mesenchymal stem cell transplantation are both practical effective methods for the treatment of cerebral ischemia. However, whether there is a synergistic effect between two remains unclear. In this study, we established rat models ischemia/reperfusion injury by occlusion middle artery 2 hours reperfusion 24 hours. Rat were perfused with bone marrow cell-derived exosomes (MSC-exos) via tail vein underwent 14 successive days treadmill exercise. Neurological assessment,...
The triglyceride-glucose (TyG) index is a novel biomarker of insulin resistance which might plausibly influence endogenous fibrinolysis and thus early neurological outcomes in patients with acute ischemic stroke (AIS) treated intravenous thrombolysis using recombinant tissue-plasminogen activator.We included consecutive AIS within 4.5 h symptom onset undergoing between January 2015 June 2022 this multi-center retrospective observational study. Our primary outcome was deterioration (END),...
Recent studies suggest that opioid receptor signaling may differentially affect Alzheimer's disease (AD) pathology and the relevant behavioral dysfunctions. However, precise roles mechanisms of subtypes in AD pathologies are still unclear with major controversies. We compared delta-opioid (DOR)- mu-opioid (MOR)-mediated effects on AD-associated cognitive deficits, pathologies, neuroinflammations, cell death using transgenic APP/PS1 mouse model BV2 line at behavioral, molecular, cellular...
Background Symptomatic intracerebral hemorrhage (sICH) after intravenous recombinant tissue plasminogen activator in patients with acute ischemic stroke (AIS) remains a feared yet unpredictable complication. We aimed to develop and validate new predictive model incorporating clinical variables noncontrast head computed tomography imaging features predict sICH AIS receiving activator. Methods Results The was derived from 808 the derivation cohort Southeast China, based on multivariable...