A5090285695- SARS-CoV-2 detection and testing
- COVID-19 diagnosis using AI
- Biosensors and Analytical Detection
- Dental Research and COVID-19
- Advanced biosensing and bioanalysis techniques
- Antibiotic Resistance in Bacteria
- Vibrio bacteria research studies
- Legionella and Acanthamoeba research
- Reproductive tract infections research
- Acute Lymphoblastic Leukemia research
- SARS-CoV-2 and COVID-19 Research
- Urinary Tract Infections Management
- Pharmaceutical and Antibiotic Environmental Impacts
- Heme Oxygenase-1 and Carbon Monoxide
- Embedded Systems and FPGA Design
- vaccines and immunoinformatics approaches
- Infections and bacterial resistance
- Childhood Cancer Survivors' Quality of Life
- Tuberculosis Research and Epidemiology
- Gut microbiota and health
- Sepsis Diagnosis and Treatment
- Reproductive System and Pregnancy
- Modular Robots and Swarm Intelligence
- Chronic Myeloid Leukemia Treatments
- Antibiotics Pharmacokinetics and Efficacy
Hokkaido University Hospital
2012-2024
Japan Anti Tuberculosis Association
2020
Health Sciences University of Hokkaido
2015
Hokkaido University
2010-2015
Keio University
2002
Rapid detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is critical for the diagnosis disease 2019 (COVID-19) and preventing spread virus. A novel kit – Novel Coronavirus Detection Kit (nCoV-DK) halves time by eliminating steps RNA extraction purification. We evaluated concordance between nCoV-DK direct PCR. The virus was detected in 53/71 specimens (74.6%) PCR 55/71 (77.5%) nCoV-DK; overall rate 94.4%: 95.2% nasopharyngeal swab, 95.5% saliva, 85.7% sputum. test...
We prospectively compared the efficacy of PCR detection SARS-CoV-2 between paired nasopharyngeal and saliva samples in 76 patients including ten COVID-19 patients. The overall concordance rate virus two was 97.4% (95%CI, 90.8-99.7). Viral load equivalent patients, but tended to disappear earlier at convalescent phase samples. These results suggest that is a reliable noninvasive alternative swabs facilitate widespread testing face shortages protective equipment without posing risk healthcare workers.
The mechanism by which Escherichia coli acquires multidrug resistance genes from other bacteria in the natural environment or livestock is still unclear. ability of ciliates to promote transfer encoding extended-spectrum β-lactamases (ESBLs) between CTX-M-27 donor and clinically isolated recipient E. strains was investigated. Equal amounts (∼109 cfu) cefotaxime-resistant ciprofloxacin-resistant were mixed together presence absence 105 Page's amoeba saline for 24 h, certain drugs...
Parachlamydia acanthamoebae is an obligately intracellular bacterium that infects free-living amoebae and a potential human pathogen in hospital-acquired pneumonia. We examined whether the presence of P. related to Acanthamoeba actual hospital environment assessed vitro survival acanthamoebae. Ninety smear samples were collected between November 2007 March 2008 (trial 1, n = 52) October February 2009 2, 38) from floor (dry conditions, 56) sink outlets (moist 34) hospital. The prevalences DNA...
Ancient chlamydiae diverged into pathogenic and environmental 0.7-1.4 billion years ago. However, how adapted to mammalian cells that provide a stable niche at approximately 37 °C, remains unknown, although have evolved as endosymbionts of lower eukaryotes in harsh niches relatively low temperatures. Hence, we assessed whether an chlamydia, Parachlamydia Bn9, could grow human HEp-2 culture temperature 30 °C. The assessment inclusion formation by quantitative RT-PCR revealed the numbers...
Four tuberculosis (TB) diagnostic health facilities of the Chongwe District, Zambia.To determine frequency bacteriologically confirmed TB patients lost to follow-up (LTFU) before treatment from January December 2017.This is a retrospective cohort study involving review registers. Information on presumptive who tested positive either by smear microscopy or Xpert® MTB/RIF assay was extracted laboratory registers and cross-matched with facilities.Two hundred seventeen were found in Of these,...
The Omicron emerged in November 2021 and became the predominant SARS-CoV-2 variant globally. It spreads more rapidly than ancestral lineages its rapid detection is critical for prevention of disease outbreaks. Antigen tests such as immunochromatographic assay (ICA) chemiluminescent enzyme immunoassay (CLEIA) yield results quickly standard polymerase chain reaction (PCR). However, their utility remains unclear. We herein evaluated performance ICA CLEIA saliva from 51 patients with 60 PCR...
Intragenic deletion of IKZF1 is a recurrent genomic alteration in acute lymphoblastic leukemia. The deletions are mediated by illegitimate variable(diversity)joining recombination via cryptic signal sequences (RSSs). We developed fluorescence situ hybridization (FISH) probe set that can detect any type deletion, including the commonly deleted exon 4 to 7 region. consists designed for region (Cy3; red) and bacterial artificial chromosomes clone detecting 3' flanking (Spectrum Green). Intact...
Abstract Rapid detection of SARS-CoV-2 is critical for the diagnosis coronavirus disease 2019 (COVID-19) and preventing spread virus. A novel “2019 Novel Coronavirus Detection Kit (nCoV-DK)” halves time by eliminating steps RNA extraction purification. We evaluated concordance between nCoV-DK direct PCR. The virus was detected in 53/71 fresh samples method 55/71 corresponding frozen nCoV-DK. overall rate two methods 94.4% (95% CI, 86.2-98.4). Concordance rates were 95.2% 83.8-99.4), 95.5%...
Rapid characterization of the causative agent(s) during a disease outbreak can aid in implementation effective control measures. However, isolation from crude clinical samples be challenging and time-consuming, hindering establishment countermeasures. In present study, we used saliva specimens collected for diagnosis SARS-CoV-2-a good example practical target-and attempted to characterize virus within without isolation. Thirty-four coronavirus 2019 patients were extract RNA synthesize DNA...
We have previously demonstrated conjugation of Escherichia coli into vacuoles the protozoal ciliate (Tetrahymena thermophila). This indicated a possible role ciliates in evoking bacterial quorum sensing, directly connecting survival via accumulation vacuoles. therefore assessed if promoted autoinducer (AI)-2 with vacuole formation, which controls sensing. E. AI-2 was significantly enhanced supernatants mixed culture and bacteria, likely depending on density rather than concentration. As...
The Omicron is the predominant SARS-CoV-2 variant globally. It spreads more rapidly than ancestral lineages and its rapid detection critical for prevention of disease outbreaks. Antigen tests such as immunochromatographic assay (ICA) chemiluminescent enzyme immunoassay (CLEIA) yield results quickly standard polymerase chain reaction (PCR). However, their utility remains unclear. We herein evaluated performance ICA CLEIA in 51 patients with 60 negative individuals. Sensitivity specificity...
A total of 713 strains fecal Escherichia coli (E. coli) isolated from laboratory animals in the colonies 4 research laboratories and commercial breeders Japan 1994 were examined regard to resistance 8 antibacterial agents. The incidence sulfadimethoxine (Su), streptomycin (Sm), ampicillin, cephaloridine, tetracycline, chloramphenicol, kanamycin, gentamicin was 99.9%, 32.5%, 6.7%, 0.7%, 7.0%, 2.6%, 6.6% respectively. These results indicated that Su Sm are penetrating into normal E. animals.