A5025073733- Immunotherapy and Immune Responses
- Immune Cell Function and Interaction
- CAR-T cell therapy research
- T-cell and B-cell Immunology
- Mesenchymal stem cell research
- Asthma and respiratory diseases
- Cancer Immunotherapy and Biomarkers
- RNA Interference and Gene Delivery
- Neuropeptides and Animal Physiology
- Monoclonal and Polyclonal Antibodies Research
- SARS-CoV-2 and COVID-19 Research
- Cancer, Stress, Anesthesia, and Immune Response
- Cancer Research and Treatments
- Virus-based gene therapy research
- Histone Deacetylase Inhibitors Research
- Cytokine Signaling Pathways and Interactions
- MicroRNA in disease regulation
- Ion Channels and Receptors
- Extracellular vesicles in disease
- IL-33, ST2, and ILC Pathways
- Immune cells in cancer
- Protein Degradation and Inhibitors
- Dermatology and Skin Diseases
- Immune Response and Inflammation
- interferon and immune responses
Université de Montréal
2016-2025
Karolinska Institutet
2024
McGill University
2007-2023
IntelliView Technologies (Canada)
2020
Lebanese University
2018
Institute for Research in Immunology and Cancer
2007-2012
Jewish General Hospital
2006-2009
Abstract The administration of ex vivo culture-expanded mesenchymal stromal cells (MSCs) has been shown to reverse symptomatic neuroinflammation observed in experimental autoimmune encephalomyelitis (EAE). mechanism by which this therapeutic effect occurs remains unknown. In an effort decipher MSC mode action, we found that conditioned medium inhibits EAE-derived CD4 T cell activation suppressing STAT3 phosphorylation via MSC-derived CCL2. Further analysis demonstrates the is dependent on...
Abstract Solid tumours are innervated by nerve fibres that arise from the autonomic and sensory peripheral nervous systems 1–5 . Whether neo-innervation of pain-initiating neurons affects cancer immunosurveillance remains unclear. Here we show melanoma cells interact with nociceptor neurons, leading to increases in their neurite outgrowth, responsiveness noxious ligands neuropeptide release. Calcitonin gene-related peptide (CGRP)—one such nociceptor-produced neuropeptide—directly exhaustion...
The use of allogeneic "universal donor" mesenchymal stromal cells (MSCs) may be a substantial clinical convenience for treatment autoimmune ailments such as multiple sclerosis. We therefore tested whether MSCs can exploited experimental encephalomyelitis (EAE) in mice with otherwise intact immune system. Administration Balb/c-derived to C57Bl/6 pre-established EAE led significant decrease disease score over time comparable that achieved syngeneic MSCs, and was correlated blunting cell...
Knee osteoarthritis (OA) is a debilitating condition affecting human body biomechanics and quality of life. Current standard care for knee OA leads to trivial improvement entails multiple adverse effects or complications. Recently, investigational cell therapies injected intra-articularly, such as bone marrow aspirate concentrate (BMAC) platelet-rich plasma (PRP), have shown safety therapeutic potency providing patients with pain relief. In the current retrospective comparative study, we...
Abstract Worldwide prevalence of cervical cancer decreased significantly with the use human papilloma virus (HPV)‐targeted prophylactic vaccines. However, these multivalent antiviral vaccines are inert against established tumors, which leave patients surgical ablative options possibly resulting in long‐term reproductive complications and morbidity. In an attempt to bypass this unmet medical need, we designed a new E7 protein‐based vaccine formulation using Accum™, technology platform promote...
Evolution has created complex mechanisms to sense environmental danger and protect tissues, with the nervous immune systems playing pivotal roles. These work together, coordinating local systemic reflexes restore homeostasis in response tissue injury infection. By sharing receptors ligands, they influence pathogenesis of various diseases. Recently, a less-explored aspect neuroimmune communication emerged: release neuropeptides from cells cytokines/chemokines sensory neurons. This article...
Cardiac glycosides are approved for the treatment of heart failure as Na+/K+ pump inhibitors. Their repurposing in oncology is currently investigated preclinical and clinical studies. However, identification a specific cancer type defined by molecular signature to design targeted trials with cardiac remains be characterized. Here, we demonstrate that glycoside proscillaridin A specifically targets MYC overexpressing leukemia cells stem causing degradation, epigenetic reprogramming...
The cross-presenting capacity of dendritic cells (DCs) can be limited by non-specific degradation during endosome maturation. To bypass this limitation, we present in study a new Accum-based formulation designed to promote endosome-to-cytosol escape. Treatment primary DCs with Accum linked the xenoantigen ovalbumin (OVA) triggers endosomal damages and enhances protein processing. Despite multiple challenges using ascending doses tumor cells, DC prophylactic vaccination results complete...
Given recent leverage of mesenchymal stromal cells (MSCs) as a potent vaccination platform, we investigated whether forced degradation an expressed experimental antigen fused to small degron sequences could prime antitumoral responses. Retrovirally gene-engineered MSCs were evaluated for their in-vitro presentation capacity, nature generated peptide repertoire and therapeutic potency in syngeneic immunocompetent mice with pre-established solid T cell lymphoma. Despite lack noticeable changes...
<title>Abstract</title> <bold>Background:</bold> In addition to triggering endosomal escape, the Accum<sup>®</sup> platform was recently reported for its ability instill antigen cross-presentation properties in mesenchymal stromal cells (MSCs). Despite promising results obtained with first-generation vaccine using A1 derivative (ARM vaccine), large quantities of cancer antigens were required achieve meaningful therapeutic effects. Given this limitation, additional variants engineered and...
Summary The vaccination efficacy in the elderly is significantly reduced compared to younger populations due thymic involution and age‐related intrinsic changes affecting their naïve T‐cell compartment. Interleukin ( IL )‐21 was recently shown display thymostimulatory properties. Therefore, we hypothesized that its administration ageing hosts may improve output thus restore a competent peripheral Indeed, an increase production of recent emigrants RTE s) attributable intrathymic expansion...
Dendritic cells (DCs) excel at cross-presenting antigens, but their effectiveness as cancer vaccine is limited. Here, we describe a vaccination approach using mesenchymal stromal (MSCs) engineered to express the immunoproteasome complex (MSC-IPr). Such modification instills efficient antigen cross-presentation abilities associated with enhanced major histocompatibility class I and CD80 expression, de novo production of interleukin-12, higher chemokine secretion. This capacity MSC-IPr highly...
Abstract Background Mesenchymal stromal cells (MSCs) have been extensively used in the clinic due to their exquisite tissue repair capacity. However, they also hold promise field of cellular vaccination as can behave conditional antigen presenting response interferon (IFN)-gamma treatment under a specific regimen. This suggests that immune function MSCs be pharmacologically modulated. Given capacity agonist pyrimido-indole derivative UM171a trigger expression various presentation-related...
ABSTRACT Vagal sensory neurons convey sensations from internal organs along the vagus nerve to brainstem. Pruriceptors are a subtype of that transmit itch and induce pruritus. Despite extensive research on molecular mechanisms itch, studies focusing pruriceptors in vagal ganglia still need be explored. In this study, we characterized pruriceptor by their responsiveness pruritogens such as lysophosphatidic acid, β -alanine, chloroquine, cytokine oncostatin M. We discovered lung-resident...
Autologous bone marrow mesenchymal stromal cells (MSCs) have been successfully used for the delivery of erythropoietin (EPO) in murine models anemia and myocardial infarction. For clinical applications where a transient effect would be adequate, such as infarction, use EPO-engineered universal donor allogeneic MSCs substantial convenience. We thus investigated whether from C57BL/6 mice permit robust EPO normal BALB/c allorecipients. Implantation overexpressing led to increases hematocrit...