A5101573389- Pluripotent Stem Cells Research
- Nanoparticles: synthesis and applications
- Heat shock proteins research
- Endoplasmic Reticulum Stress and Disease
- Ubiquitin and proteasome pathways
- Anesthesia and Neurotoxicity Research
- Graphene and Nanomaterials Applications
- RNA Research and Splicing
- DNA Repair Mechanisms
- CRISPR and Genetic Engineering
- Porphyrin and Phthalocyanine Chemistry
- Extracellular vesicles in disease
- Neuroscience and Neural Engineering
- Photodynamic Therapy Research Studies
- Neuroinflammation and Neurodegeneration Mechanisms
- Acute Myeloid Leukemia Research
- RNA Interference and Gene Delivery
- Neurogenesis and neuroplasticity mechanisms
- Plant Virus Research Studies
- Alzheimer's disease research and treatments
- 3D Printing in Biomedical Research
- Immune cells in cancer
- Metal complexes synthesis and properties
- Microtubule and mitosis dynamics
- MicroRNA in disease regulation
Zhejiang University
2021-2025
Western University of Health Sciences
2015-2023
National Taiwan University
2023
Fu Jen Catholic University
2023
University of Dayton
2007-2013
Albert B. Chandler Hospital
1999-2008
University of Kentucky
1996-2007
University of Cincinnati Medical Center
2003-2006
University of Cincinnati
2005-2006
University of California, Davis
2005
Carbon nanotubes (CNTs) have shown promise as an important new class of multifunctional building blocks and innovative tools in a large variety applications, ranging from nanocomposite materials through nanoelectronics to biomedical devices. Because their unusual one-dimensional hollow nanostructure unique physicochemical properties, CNTs are particularly useful novel drug delivery imaging agents. However, such applications will not be realized if there is no proper assessment the potential...
Heat shock transcription factor 1 (HSF1) mediates the induction of heat protein gene expression in cells exposed to elevated temperature and other stress conditions. In response HSF1 acquires DNA binding ability localizes nuclear granules, but molecular mechanisms that mediate these events are not understood. We report undergoes stress-induced modification at lysine 298 by small ubiquitin-related called SUMO-1. Antibodies against SUMO-1 supershift DNA-binding complex, a reconstituted...
Response to DNA damage and cell-cycle regulation differ markedly between embryonic stem (ES) cells somatic cells. ES require exquisitely sensitive mechanisms maintain genomic integrity do so, in part, by suppressing spontaneous mutation. Spontaneous mutation frequency is approximately 10(-4) compared with 10(-6) for also lack a G(1) checkpoint are hypersensitive IR other DNA-damaging agents. These characteristics facilitate apoptosis the removal of mutational burden from population, thereby...
Microglia play an important role in neuroinflammation and neurodegeneration. Here, we report approach for generating microglia-containing cerebral organoids derived from human pluripotent stem cells involving the supplementation of growth factors (FGF, EGF, heparin) 10% CO2 culture conditions. Using this platform, Western Pacific Amyotrophic Lateral Sclerosis Parkinsonism-Dementia Complex (ALS-PDC) were generated patient-derived induced (iPSCs). These ALS-PDC-affected had more reactive...
Heat shock transcription factor 2 (HSF2) is a that regulates heat protein gene expression, but the mechanisms regulating function of this are unclear. Here we report HSF2 substrate for modification by ubiquitin-related SUMO-1 and colocalizes in cells with nuclear granules. Staining anti-promyelocytic leukemia antibodies indicates these HSF2-containing granules PML bodies. Our results identify lysine 82 as major site HSF2, which located "wing" within DNA-binding domain protein. Interestingly,...
In contrast to most genomic DNA in mitotic cells, the promoter regions of some genes, such as stress-inducible hsp70i gene that codes for a heat shock protein, remain uncompacted, phenomenon called bookmarking. Here we show bookmarking is mediated by transcription factor HSF2, which binds this recruits protein phosphatase 2A, and interacts with CAP-G subunit condensin enzyme promote efficient dephosphorylation inactivation complexes vicinity, thereby preventing compaction at site. Blocking...
Silver nanoparticles (AgNPs) are among the most extensively used and found in a variety of products. This ubiquity leads to inevitable exposure these particles everyday life. However, effects AgNPs on neuron astrocyte networks still largely unknown. In this study, we neurons astrocytes derived from human embryonic stem cells as cellular model study neurotoxicity that is induced by citrate-coated (AgSCs). Immunostaining with markers, glial fibrillary acidic protein microtubule-associated...
Bisphenol-A (BPA) is a lipophilic, organic, synthetic compound that has been used as an additive in polycarbonate plastics manufacturing since 1957. Studies have shown BPA interferes with the development and functions of brain, but little known about effects on human glutamatergic neurons (hGNs) at molecular cellular levels. We investigated impact chronic exposure to hGNs derived from embryonic stem cells (hESCs). The results showed different concentrations (0, 0.1, 1.0 10 μM) for 14 days...
Silver nanoparticles (AgNPs) are gaining rapid popularity in many commonly used medical and commercial products for their unique anti-bacterial properties. The molecular mechanisms of effects AgNPs on stem cell self-renewal proliferation have not yet been well understood. aim the work is to use mouse embryonic cells (mESCs) as a cellular model evaluate toxicity AgNPs. mESC very special type which has differentiation objective this project determine with different surface chemical...
Alternative polyadenylation (APA) has been discovered to play regulatory roles in the development of many cancer cells through preferential addition a poly(A) tail at specific sites pre-mRNA. A recent study found that APA was involved mediation acute myeloid leukaemia (AML). However, unlike gene expression heterogeneity, little attention directed toward variations single-cell for different cell types during AML development. Here, we used RNA-seq data massive population 16,843 bone marrow...
Hydroxyurea (Green dots) efficiently promoted regional cerebral blood flow and increased BBB permeability. Thereby, more hypoxia-sensitive liposomes NIPP/ER@HL-D were transported to lesions entered brain exert anti-stroke efficacy.
Common neurological symptoms of COVID-19, such as anosmia, headaches, and cognitive dysfunction, depend on interactions between the peripheral central nervous systems. However, molecular mechanisms by which SARS-CoV-2 affects system remain poorly understood, with ongoing debate about whether sensory neurons can be directly infected virus. In this study, human iPSC-derived were exposed to BA.5 variant, a mutant virus, or viral S1 proteins. Under control conditions, exhibited low expression...
When the new porphyrin 5,10-(4-pyridyl)-15,20-(pentafluorophenyl)porphyrin is reacted with 2 equiv of Ru(bipy)2Cl2 (where bipy = 2,2′-bipyridine) formation target ruthenated achieved 40% yield. Strong electronic transitions are observed in visible region spectrum associated Soret and four Q-bands. A shoulder at slightly higher energy than band attributed to Ru(dπ) bipy(π*) metal ligand charge transfer (MLCT) band. The bipyridyl π π* transition occurs 295 nm. Cyclic voltammetry experiments...
Coordination of two [Ru(bipy)2Cl]+ moieties (where bipy = 2,2′-bipyridine) to the pyridyl nitrogens in 5,10-positions meso-5,10,15-(4-pyridyl)-20-(pentafluorophenyl)porphyrin gives diruthenium porphyrin complex I. Insertion nickel(II), copper(II), and zinc(II) into center complexes II–IV, respectively. Electronic transitions associated with ruthenium include an intense Soret band four less Q-bands visible region spectrum. An π–π* transition UV bipyridyl groups a metal-to-ligand charge...
Stem cell-based neuronal differentiation has provided a unique opportunity for disease modeling and regenerative medicine. Neurospheres are the most commonly used neuroprogenitors differentiation, but they often clump in culture, which always represented challenge neurodifferentiation. In this study, we report novel method defined culture conditions generating sub-type or region-specific neurons from human embryonic induced pluripotent stem cells derived neurosphere without any genetic...
We report the synthesis and characterisation of mixed-metal binuclear ruthenium(II)-vanadium(IV) complexes, which were used as potential photodynamic therapeutic agents for melanoma cell growth inhibition. The novel [Ru(pbt)2(phen2DTT)](PF6)2·1.5H2O 1 (where phen2DTT = 1,4-bis(1,10-phenanthrolin-5-ylsulfanyl)butane-2,3-diol pbt 2-(2'-pyridyl)benzothiazole) [Ru(pbt)2(tpphz)](PF6)2·3H2O 2 tpphz tetrapyrido[3,2-a:2',3'-c:3'',2''-h:2''',3'''-j]phenazine) synthesised characterised. Compound was...
Members of the phosphoprotein phosphatase family serine/threonine phosphatases are thought to exist in different native oligomeric complexes. Protein 2A (PP2A) is composed a catalytic subunit (PP2Ac) that complexes with an A subunit, which turn also interacts one many B subunits regulate substrate specificity and/or (sub)cellular localization enzyme. Another member, protein 5 (PP5), contains tetratricopeptide repeat domain at its N terminus, has been suggested mediate interactions other...