A5028760544- Advanced biosensing and bioanalysis techniques
- Biosensors and Analytical Detection
- RNA Interference and Gene Delivery
- Gold and Silver Nanoparticles Synthesis and Applications
- DNA and Nucleic Acid Chemistry
- Advanced Biosensing Techniques and Applications
- Bacteriophages and microbial interactions
- Electrochemical Analysis and Applications
- Bone Tissue Engineering Materials
- Plasmonic and Surface Plasmon Research
- Advanced Chemical Sensor Technologies
- Nanoparticle-Based Drug Delivery
- Electrospun Nanofibers in Biomedical Applications
- Fullerene Chemistry and Applications
- biodegradable polymer synthesis and properties
- Graphene research and applications
- Advanced Nanomaterials in Catalysis
- Silk-based biomaterials and applications
- Microfluidic and Bio-sensing Technologies
- MicroRNA in disease regulation
- Quantum Dots Synthesis And Properties
- Molecular Junctions and Nanostructures
- Nanopore and Nanochannel Transport Studies
- Polymer Surface Interaction Studies
- Ferroptosis and cancer prognosis
Shanghai Ocean University
2015-2025
Qinghai University
2025
Shanghai Harbour Engineering Design & Research Institute
2019-2024
Ministère de l'Agriculture et de la Souveraineté alimentaire
2019-2024
Zhengzhou University
2022-2023
BGI Group (China)
2022
Ministry of Agriculture and Rural Affairs
2019
Chengdu University
2017
National Engineering Research Center for Nanotechnology
2013-2017
Shanghai Institute of Applied Physics
2008-2015
There remains a great challenge in the sensitive detection of microRNA because short length and low abundance microRNAs cells. Here, we have demonstrated an ultrasensitive platform for by combining tetrahedral DNA nanostructure probes hybridization chain reaction (HCR) amplification. The limits are 100 aM 10 (corresponding to 600 μL sample), respectively. Compared widely used supersandwich amplification, improved 3 orders magnitude. uncontrolled surface immobilization consumption target...
Sensitive and selective detection for cancer biomarkers are critical in clinical diagnostics. Here we developed a microfluidic protein chip an ultrasensitive multiplexed assay of biomarkers. Aqueous-phase-synthesized CdTe/CdS quantum dots (aqQDs) were employed as fluorescent signal amplifiers to improve the sensitivity. Secondary antibodies (goat anti-mouse IgG) conjugated luminescent QDs realize versatile probe that could be used both sandwich reverse phase immunoassays. We found our not...
We have developed a surface-enhanced Raman scattering (SERS)-active substrate based on gold nanoparticle-decorated chemical vapor deposition (CVD)-growth graphene and used it for multiplexing detection of DNA. Due to the combination nanoparticles graphene, signals dye were dramatically enhanced by this novel substrate. With nanoparticles, DNA capture probes could be easily assembled surface films which drawback directly immobilize This platform exhibits extraordinarily high sensitivity...
Several single-stranded scaffold DNA, obtained from rolling circle amplification (RCA), are folded by different staples to form DNA nanoribbons. These nanoribbons rigid, simple design, and cost-effective drug carriers, which readily internalized mammalian cells show enhanced immunostimulatory activity. As a service our authors readers, this journal provides supporting information supplied the authors. Such materials peer reviewed may be re-organized for online delivery, but not copy-edited...
DNA self-assembling nanostructure has been considered as a promising candidate drug delivery vehicle because of its compactness, mechanical stability, and noncytotoxicity. In this work, we developed functional, multiform nanostructures by appending tumor-penetrating peptide to tetrahedral (p-TDN). This functional structure is able efficiently increase the rate uptake glioblastoma cell U87MG compared with tetrahedron double-stranded structures. We found that plays main role in endocytosis...
Molecular computing holds great promise for diagnosis and treatment of diseases at the molecular level; nevertheless, designing logic gates to operate programmably autonomously diagnostics still remains challenging. We designed on DNA Origami microRNA analysis. As a demonstration, two indicators heart failure, microRNA-21 microRNA-195, were selected as inputs. The contain main modules: computation module output module, performing in single nanostructure. recognizes disease indicators, while...
Abstract A novel three‐dimensional (3D) superstructure based on the growth and origami folding of DNA gold nanoparticles (AuNPs) was developed. The 3D contains a nanoparticle core dozens two‐dimensional belts folded from long single‐stranded DNAs grown in situ by rolling circle amplification (RCA). We designed two mechanisms to achieve loading molecules onto superstructures. In one mechanism, ligands bound target are merged into growing during RCA process (merging mechanism). other...
Sensitive and rapid detection of pathogenic bacteria is essential for effective source control prevention microbial infectious diseases. However, it remains a substantial challenge to rapidly detect at the single-cell level. Herein, we present an electrochemical sandwich sensor highly selective ultrasensitive single bacterial cell based on dual recognition by bacteria-imprinted polymer film (BIF) aptamer. The BIF was used as capture probe, which in situ fabricated electrode surface within 15...
Cellular imaging technologies employing metallic surface-enhanced Raman scattering (SERS) tags have gained much interest toward clinical diagnostics, but they are still suffering from poor controlled distribution of hot spots and reproducibility SERS signals. Here, we report the fabrication characterization high narrow nanogap-containing Au@Au core-shell nanoparticles (GCNPs) for identification proteins overexpressed on surface cancer cells. First, plasmonic nanostructures made gold (∼15 nm)...
It is critical to detect and analyze the heavy metal pollutions in environments foods. Chemosensors have been widely investigated for fast detection of analytes such as metals due their unique advantages. In order improve sensitivity chemosensors, recently electrospun nanofibrous membranes (ENMs) explored immobilization chemosensors or receptors high surface‐to‐volume ratio, porosity, easiness fabrication functionalization, controllability nanofiber properties, low cost, easy detection, no...
The effective identification and quantification of pathogenic bacteria is essential for addressing serious public health issues. Here, we demonstrate a simple universal impedimetric sensor highly selective sensitive detection based on the recognition by bacteria-imprinted polypyrrole (BIP) film. BIP film was facilely prepared via one-step electro-polymerization followed in situ removal bacterial template. structure novel with noncavity-like imprinted sites situated at surface (PPy) matrix,...
The spatial arrangement of DNA probes on the electrode surface is critical significance for performance electrochemical biosensors. However, rational control probe remains challenging. In this work, we develop a capture carrying poly-adenine anchoring block to construct programmable self-assembled monolayer "sandwich-type" biosensor. We show that with co-assembling strategy using polyA and 6-mercapto-1-hexanol, density gold can be simply adjusted by length polyA. electron-transfer effect...
Although "hot spots" have been proved to contribute surface enhanced Raman scattering (SERS), less attention was paid increase the number of spot" directly enhance signals in bioanalytical systems. Here we report a new strategy based on nano rolling-circle amplification (nanoRCA) and hyperbranched (nanoHRCA) groups for protein microarrays. First, ssDNA are coassembled gold nanoparticles, making assembled probe both binding ability hybridization ability. Second, ssDNAs act as primers initiate...
The ability of drug release from SF materials was governed largely by their secondary structure. It is known that the breakage degree peptide chain during silk fibroin (SF) dissolution can affect structure, property, and applications materials. To deeply understand this effect, we designed a reaction system based on CaCl2/H2O/C2H5OH ternary solvent with different ethanol content to obtain regenerated films morphologies structures. results showed globule-like nanostructure observed in all...
The development of electrochemical DNA biosensors has been limited by their reliability and reproducibility due to many interfering factors such as electrode properties, surface densities, complex biological samples. In this work, we developed a nanobalance polyA hairpin probe (polyA-HP), which was effectively assembled onto the gold through affinity between central fragment Au surface. One flanking polyA-HP captured target sequence together with MB-labeled signal probe, other reference...