A5100462397- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- RNA modifications and cancer
- Galectins and Cancer Biology
- Glycosylation and Glycoproteins Research
- Colorectal Cancer Treatments and Studies
- RNA Research and Splicing
- Circular RNAs in diseases
- Carbohydrate Chemistry and Synthesis
- Cancer Cells and Metastasis
- Glioma Diagnosis and Treatment
- Ginger and Zingiberaceae research
- Caveolin-1 and cellular processes
- Epigenetics and DNA Methylation
- Tryptophan and brain disorders
- Genetic factors in colorectal cancer
- Cancer Genomics and Diagnostics
- Neuroendocrine regulation and behavior
- Curcumin's Biomedical Applications
- Cancer Research and Treatments
- Neuroblastoma Research and Treatments
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Lung Cancer Treatments and Mutations
- Cancer-related Molecular Pathways
- CAR-T cell therapy research
Tianjin Medical University
2024
Hangzhou Normal University
2024
Nanjing Medical University
2024
Capital Medical University
2013-2022
Beijing Chao-Yang Hospital
2016-2022
First Affiliated Hospital of Xi'an Jiaotong University
2021
Yang Hospital
2020
China Medical University
2015-2019
First Hospital of China Medical University
2015-2019
Beijing Anzhen Hospital
2017
// Qian Wang 1 , Lei Yang 3 Xin Hu Yuliang Jiang Yizhang Zhe Liu Jian Tao Wen Yingmin Ma 2 Guangyu An Guosheng Feng Department of Oncology, Affiliated Beijing Chaoyang Hospital, Capital Medical University, 100020, China Respiratory Medicine, Research Center, Correspondence to: Feng, email: fengguoshengcyh@163.com An, anguangyucyh@163.com Ma, mayingmincyh@163.com Keywords: long non-coding RNA, NNT-AS1, colorectal cancer, MAPK/Erk, biomarker Received: April 17, 2016 Accepted: November 21,...
microRNAs are aberrantly expressed during the development and progression of a variety human cancers, including colorectal cancer (CRC). Of these microRNAs, microRNA-375 (miR-375) was previously observed to be downregulated in cancer(CRC) plasma tissues, but its functions largely unknown. Here, we investigated impact miR-375 on CRC metastasis. Specifically, expression significantly decreased tissues compared with their matched noncancerous (NCTs), low levels predicted tumor metastatic...
Aberrant O-glycosylation is frequently observed in colorectal cancer (CRC) patients, but it unclear if contributes intrinsically to tumorigenesis. Here, we investigated the biological consequences of aberrant CRC. We first detected expression profile Tn antigen a serial human CRC tissues and then explored genetic biosynthetic mechanisms. Moreover, used cell line (LS174T), which express antigen, assess whether can directly promote oncogenic properties. It showed that was around 86% primary...
Abstract Tn antigen is a truncated O‐glycan, frequently detected in colorectal cancer ( CRC ), but its precise role metastasis not well addressed. Here we investigated the effects of Core 1 β3Gal‐T specific molecular chaperone (Cosmc) deletion‐mediated exposure on and underlying mechanism. We first used CRISPR /Cas9 technology to knockout Cosmc, which required for normal O‐glycosylation, thereby obtained Tn‐positive cells. then biological consequences expression . The results showed that...
To detect the expression profiles of microRNA-218 (miR-218) in human pancreatic cancer tissue (PCT) and cells their effects on biological features cell line PANC-1 observe effect miR-218 target gene high mobility group box 1 (HMGB1), with an attempt to provide new treatment methods strategies for cancer.The expressions PCT normal pancreas as well various lines including AsPC-1, BxPC-3, were determined quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR). The...
Abstract HACE1, an E3 ubiquitin-protein ligase, is frequently inactivated and has been evidenced as a putative tumor suppressor in different types of cancer. However, its role glioma remains elusive. Here, we observed increased expression HACE1 gliomas related to control subjects, found strong correlation high with poor prognosis patients WHO grade III IV well low-grade (LGG) receiving radiotherapy. knockdown obviously suppressed malignant behaviors cells, while ectopic enhanced cell growth...
Immature truncated O-glycans such as Tn antigen are frequently detected in human colorectal cancer (CRC); however, the precise pathological consequences of expression on CRC unknown. T-synthase is key enzyme required for biosynthesis mature O-glycans. Here we investigated functional roles mediated by deficiency cells.To knock out T-synthase, used CRISPR-Cas9 technology to target C1GALT1, gene encoding a cell line (HCT116). Deletion was confirmed western blotting, and determined flow...
Elemene is an effective anticancer component extracted from Zingiberaceae plants. This work was aimed to evaluate the anti-tumor effect and mechanism actions of elemene on pancreatic carcinoma in vitro vivo. The anti-proliferation experiment measured by Methylthiazolyldiphenyl-tetrazolium bromide (MTT) method time 24, 48 72 h three different dosages. cell cycle detected flow cytometer after 12 treatment. Forty-eight nude mice were subcutaneously xenograft with BxPC-3 cancer cells divided...
Nicotinamide nucleotide transhydrogenase-antisense RNA1 (NNT-AS1) is a long noncoding RNA (lncRNA) that has been shown to be overexpressed in hepatocellular carcinoma (HCC). However, the molecular mechanism involving NNT-AS1 HCC remains extensively investigated. The activation of TGF-β signaling inhibits tumor-infiltrating lymphocytes (TILs) and results tumor immune evasion. We thus planned explore by which activates pathway TILs HCC. High levels were detected tissues both RNAscope real-time...
Circular RNAs (circRNAs) play an important role in cholangiocarcinoma (CCA) development; however, the expression and functions of circRNAs distal CCA (dCCA) remain unknown. Herein, we explored profile six paired dCCA tumor adjacent normal tissue samples using microarray. A total 171 differentially expressed (DE) were identified tissues. Host genes DE enriched cellular cytoskeleton adheren junction. Bioinformatics analyses used to establish a circRNA-microRNA-mRNA network for dCCA....
Micro(mi)RNAs play an essential role in the epithelial-mesenchymal transition (EMT) process human cancers. This study aimed to uncover regulatory mechanism of miR-1301-3p on EMT pancreatic cancer (PC). The miRNA profilings from Gene Expression Omnibus data sets (GSE31568, GSE41372, and GSE32688) demonstrated downregulation PC tissues, which was validated with 72 paired tissue samples through qRT-PCR detection. low level associated a poor prognosis for patients cohort Cancer Genome Atlas...
ABSTRACT Colorectal cancer is the fifth prevalent in China. Nevertheless, a large-scale characterization of Chinese colorectal mutation spectrum has not been carried out. In this study, we have performed whole exome-sequencing analysis 98 patients’ tumor samples with matched pairs normal colon tissues using Illumina and Complete Genomics high-throughput sequencing platforms. Canonical CRC somatic gene mutations high prevalence (>10%) verified, including TP53, APC, KRAS, SMAD4, FBXW7...
To analyze the weight change trajectory in patients with locally advanced nasopharyngeal carcinoma (LANPC) before, during, and after radiation therapy for a time span of 40 weeks.147 from university-affiliated medical center China were included.Body was measured weekly during intensive treatment biweekly therapy.All 147 experienced critical loss peri-radiation period. Overall, body remained basically unchanged induction chemotherapy, followed by sharp severe decrease therapy. At 20 weeks...
Abstract Micro(mi)RNAs play an essential role in the epithelial-mesenchymal transition (EMT) process human cancers. This study aimed to uncover regulatory mechanism of miR-1301-3p on EMT pancreatic cancer (PC). The miRNA profilings from Gene Expression Omnibus datasets (GSE31568, GSE41372, and GSE32688) demonstrated downregulation PC tissues, which was validated with 72 paired tissue samples through qRT-PCR detection. low level associated a poor prognosis for patients cohort Cancer Genome...
Abstract Background: Micro(mi)RNAs play an essential role in the epithelial-mesenchymal transition (EMT) process human cancers. This study aimed to uncover regulatory mechanism of miR-1301-3p on EMT pancreatic cancer (PC). Methods: GEO database (GSE31568, GSE41372, and GSE32688) PC cohort The Cancer Genome Atlas were applied discover expression prognostic miR-1301-3p. In validation cohort, qRT-PCR was performed 72 paired tissue samples. CCK-8, wound healing, transwell migration assays used...
Abstract Background Nicotinamide Nucleotide Transhydrogenase-antisense RNA1 (NNT-AS1) is one of long non coding RNA, has been shown with high levels in several types cancers. However, the molecular mechanism remains to be revealed for NNT-AS1 progession hepatocellular carcinoma (HCC). TGF-β signaling pathway identified as negative factor excessive immunological response. We sought investigate effects mediating T cells infiltration by regulating patients HCC. Methods RNAscope In Situ...
Backgrounds: Indoleamine 2,3-dioxygenase (IDO) has previously been elucidated in tolerant cardiac or liver transplantation models. Kynurenine also reported immune Immutol is an analogue of IDO and metabolites, it anthranilic acid derivative a synthetic chemical. Its function rat remains unknown. Here, we the role mechanism combine with CsA transplantation. Methods: Using model intervented by Immutol, CsA, Immutol+CsA before surgery. Moreover, used 1-MT, inhibitory, to furtherly validate...