A5113518245- Pluripotent Stem Cells Research
- Mesenchymal stem cell research
- Pancreatic function and diabetes
- Neurogenesis and neuroplasticity mechanisms
- CRISPR and Genetic Engineering
- RNA Research and Splicing
- Cancer Research and Treatments
- Renal and related cancers
- MicroRNA in disease regulation
- Liver physiology and pathology
- Osteoarthritis Treatment and Mechanisms
- Biomedical Ethics and Regulation
- RNA Interference and Gene Delivery
- 3D Printing in Biomedical Research
- Corneal Surgery and Treatments
- Congenital heart defects research
- Epigenetics and DNA Methylation
- Tissue Engineering and Regenerative Medicine
- Cancer-related gene regulation
- Cell Adhesion Molecules Research
- Glioma Diagnosis and Treatment
- Connexins and lens biology
- Ocular Surface and Contact Lens
- Angiogenesis and VEGF in Cancer
- Neuroscience and Neural Engineering
Chinese Academy of Sciences
2013-2024
Shanghai Advanced Research Institute
2012-2024
University of Chinese Academy of Sciences
2014-2022
Henan Provincial People's Hospital
2022
Zhengzhou University
2022
Guangxi Medical University
2020
Peking University
2008-2017
Shanghai Institute of Materia Medica
2017
ShanghaiTech University
2017
Peking University Third Hospital
2006
Abstract To examine whether transplantation of human mesenchymal stem cells (MSCs) could reconstruct the corneal damage and also grafted MSCs differentiate into epithelial cells, we isolated from healthy donors. After growth expansion on amniotic membrane, were transplanted rat corneas 7 days after chemical burns. Reconstruction damaged cornea vision measured once a week by slit lamp an optokinetic head-tracking instrument, respectively. Corneas then cut out, fixed, imbedded for...
Abstract OCT4 is a pivotal transcription factor in maintaining the pluripotency and self-renewal capacities of embryonic stem (ES) cells. Human can generate two isoforms by alternative splicing, termed OCT4A OCT4B. confers stemness properties ES cells, whereas function OCT4B unknown. We present here diverse protein products novel gene. A single mRNA encode three translation initiation at AUG CUG start codons, respectively. putative internal ribosome entry site (IRES) has been identified...
The transcriptional factor Sox2 and epidermal growth receptor (Egfr)-mediated signaling are both required for self-renewal of neural precursor cells (NPCs). However, the mechanism by which these factors coordinately regulate this process is largely unknown. Here we show that Egfr-mediated promotes expression, in turn binds to Egfr promoter directly upregulates expression. Knockdown RNA interference downregulates expression attenuates colony formation NPCs, whereas overexpression elevates NPC...
Abstract Lung fibrosis is characterized by vascular leakage and myofibroblast recruitment, both phenomena are mediated lysophosphatidic acid ( LPA ) via its type‐1 receptor 1). Following lung damage, the accumulated myofibroblasts activate secrete excessive extracellular matrix ECM ), form fibrotic foci. Studies have shown that bone marrow‐derived cells an important source of in organ. However, type marrow contributing predominantly to involvement ‐ 1 signalling this yet unclear. Using a...
The transcription factor Sox2 [SRY (sex-determining region Y)-box 2] is essential for the regulation of self-renewal and homoeostasis NSCs (neural stem cells) during brain development. However, downstream targets its underlying molecular mechanism are largely unknown. In present study, we found that directly up-regulates expression survivin, which inhibits mitochondria-dependent apoptotic pathway in NSCs. Although overexpression elevates survivin expression, knockdown results a decrease...
Human induced pluripotent stem cells (hiPSCs) can proliferate infinitely. Their ability to differentiate into cardiomyocytes provides abundant sources for disease modeling, drug screening and regenerative medicine. However, hiPSC-derived (hiPSC-CMs) display a low degree of maturation fetal-like properties. Current in vitro differentiation methods do not mimic the structural, mechanical, or physiological properties cardiogenesis niche. Recently, we present an efficient cardiac platform that...
Summary Stem cell transplantation is a promising treatment for many conditions. Although stem cells can be isolated from tissues, blood the ideal source of these due to ease collection. Mesenchymal (MSCs) have been paid increased attention because their powerful proliferation and pluripotent differentiating ability. But whether MSCs reside in (newborn umbilical cord fetal or adult peripheral blood) also debatable. The present study showed that MSC‐like could expanded 16–26 weeks but were not...
Pax6 is a key regulator in the neuronal fate determination as well proliferation of neural stem cells, but mechanisms are still unknown. Our study shows that regulate progenitor cells cortical subventricular zone, through direct modulation Sox2 expression during late developmental stage mice. We found dramatic decrease number Sox2+ zone E18.5 Pax6(-/-) confirmed could bind to promoter by chromatin immunoprecipitation assay and activate luciferase reporter gene assay. Moreover, progenitors...
Monoamine oxidase (MAO) is a critical metabolic enzyme of dopamine, which key neurotransmitter the mesolimbic reward pathway in human brain. Consequently, gene encoding MAO an important candidate genetics smoking behaviour. We investigated association between MAOA polymorphisms (a VNTR polymorphism and EcoRV polymorphism) status. A community-based cross-sectional study was conducted with 203 current subjects 168 non-current Beijing, China. Genotyping for these performed using PCR restriction...
Human embryonic stem (ES) cells are usually maintained in the undifferentiated state by culturing on feeder layers of mouse fibroblasts (MEFs). However, MEFs not suitable to support human ES used for clinical purpose because risk zoonosis from animal cells. Therefore, tissue-based need be developed purpose. Hereof we report that amniotic mesenchymal (hAMCs) could act as cells, they easily obtained and relatively exempt ethical problem. Like MEFs, hAMCs grow well on. The self-renewal rate...
Heterozygous paired box6 (Pax6) mutations lead to abnormal glucose metabolism in mice older than 6 months as well human beings. Our previous study found that Pax6 deficiency caused down-expression of prohormone convertase 1/3 (Pcsk1), resulting defective proinsulin processing. As a protein cleaving enzyme, addition its expression, the activity PC1/3 is closely related function. We therefore hypothesize mutation alters PC1/3, which affects processing.Using quantitative RT-PCR, western blot...
Heterozygosity for the Pax6 allele is associated with impaired glucose tolerance in humans. With a mutant mouse model, we found many of metabolic abnormalities were consistent effects down-regulating expression glucagon-like peptide 1 (GLP-1). In addition to tolerance, adult heterozygous mice (Pax6m/+) secreted less insulin responding and arginine administration compared control mice. Moreover, Pax6m/+ showed increased food intake mice, although they resistant diet-induced fat accumulation....
Highlights•Phosphorylation of threonine343 mediates global OCT4-phosphorylation (phos-OCT4T343)•Phos-OCT4T343 is crucial for OCT4 to protect embryonic stem cell pluripotency•Phos-OCT4T343 binds SOX2 but non-phos-OCT4T343 SOX17 in fate decision•Phos-OCT4T343 may regulate lineage commitment early developmentSummaryOCT4 required maintain the pluripotency cells (ESCs); yet, overdose-expression induces ESC differentiation toward primitive endoderm. The molecular mechanism underlying this switch...
Induced pluripotent stem (iPS) cells have been generated from human somatic by ectopic expression of defined transcription factors. Application this approach in may enormous potential to generate patient-specific cells. However, traditional methods reprogramming involve the use oncogenes c-MYC and KLF4, which are not applicable clinical translation. In present study, we investigated whether fetal gut mesentery-derived (hGMDCs) could be successfully reprogrammed into induced OCT4, SOX2, NANOG...