A5100715900- Neuroblastoma Research and Treatments
- melanin and skin pigmentation
- MicroRNA in disease regulation
- Biochemical Analysis and Sensing Techniques
- Skin Protection and Aging
- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- Circular RNAs in diseases
- Muscle Physiology and Disorders
- Cancer, Hypoxia, and Metabolism
- Silk-based biomaterials and applications
- Liver Disease Diagnosis and Treatment
- RNA Interference and Gene Delivery
- Hair Growth and Disorders
- Fish Ecology and Management Studies
- Adipose Tissue and Metabolism
- TGF-β signaling in diseases
- Fish Biology and Ecology Studies
- Regulation of Appetite and Obesity
- Muscle metabolism and nutrition
- Bioactive natural compounds
- Animal Genetics and Reproduction
- Cytokine Signaling Pathways and Interactions
- Ion Transport and Channel Regulation
- Mesenchymal stem cell research
Qingdao Agricultural University
2024
Chengdu University of Traditional Chinese Medicine
2023-2024
Hangzhou Center for Disease Control and Prevention
2023
Chengdu University
2023
Shanxi Agricultural University
2010-2021
Lianyungang Oriental Hospital
2020
Longgang Central Hospital
2017
Chongqing Medical University
2009-2016
Wenzhou Medical University
2011
Chinese Academy of Fishery Sciences
2009
Diabetic nephropathy (DN) is a major diabetic complication. But the initiating molecular events triggering DN are unknown. Recent researches have addressed role of microRNAs in diabetes and its complications. In this study, we looked for expression during early DN, showed microRNA‐21 (miR‐21) was downregulated response to vitro vivo. Over‐expression miR‐21 inhibited proliferation mesangial cells decreased 24‐h urine albumin excretion rate db/db mice. Moreover, identified PTEN as target...
Diabetic nephropathy (DN) is a major diabetic complication. However, the initiating molecular events triggering DN are unknown. In this study we focused on microRNA‐451 (miR‐451), which downregulated during early DN. We found that miR‐451 negatively regulated expression of Ywhaz through 3′UTR and was required for miR‐451‐mediated downregulation p38 MAPK signalling. Moreover, over‐expression inhibits glomerular mesangial cell proliferation in vitro vivo. These findings suggest...
Abstract Background Previous molecular genetic studies of physiology and pigmentation sheep skin have focused primarily on a limited number genes proteins. To identify additional that may play important roles in coat color regulation, Illumina sequencing technology was used to catalog global gene expression profiles with white versus black color. Results There were 90,006 74,533 unigenes assembled from the reads obtained skin, respectively. Genes encoding for ribosomal proteins keratin...
Down‐regulation of let‐7 microRNA (miRNA) is a key event in lung cancer. Despite recent advances survival signaling, the roles context cancer are not fully clear. In this study, we showed that let‐7a, member family, negatively regulated expression NIRF through 3′ UTR. We also was required for let‐7a‐mediated elevation p21 WAF1 . These findings suggest growth‐inhibitory effect let‐7a on A549 cells vitro and vivo may be explained part by le‐7a‐induced suppression This work reveals novel...
Abstract Background MicroRNAs (miRNAs) are small, non-coding 21–25 nt RNA molecules that play an important role in regulating gene expression. Little is known about the expression profiles and functions of miRNAs skin their pigmentation. Alpacas have more than 22 natural coat colors, any other fiber producing species. To better understand control color we performed a comprehensive analysis miRNA white versus brown alpacas. Results Two small libraries from alpaca (WA) (BA) were sequenced with...
Purpose:MYCN is one of the most well-characterized genetic markers neuroblastoma. However, mechanisms as to how MYCN mediate neuroblastoma tumorigenesis are not fully clear. Increasing evidence has confirmed that dysregulation miRNAs involved in MYCN-mediated tumorigenesis, supporting their potential therapeutic targets for Although miR-221 been reported upregulated miRNAs, interplay between and progression remains largely elusive.Experimental Design: The expression formalin-fixed,...
Tyrosinase‑related protein 2 (TRP‑2) is one of the most important members tyrosinase family, and a key enzyme involved in melanin biosynthesis. In present study, skin transcriptome profile, immunohistochemistry, western blotting reverse transcription‑quantitative polymerase chain reaction were used to investigate TRP‑2 expression sheep with different coat colors, namely, black, white black‑white. was overexpressed melanocytes order study effect on production. Results revealed differing...
Coat color is a key economic trait in wool- and fur-producing animals. controlled by complex mechanisms. Pro-opiomelanocortin (POMC) gene involved pigment formation. Previous studies suggested that miR-488 might target the POMC mRNA. This study aimed to determine whether could affect coat regulating explore regulatory roles of on mammals. A dual fluorescence report vector containing 3'-UTR was built post-transcriptionally regulate expression. Then, eukaryotic expressing transfected into...
A cDNA library from white alpaca (Vicugna pacos) skin was constructed using SMART technology to investigate the global gene expression profile in and identify genes associated with physiology of pigmentation. total 5359 high-quality EST (expressed sequence tag) sequences were generated by sequencing random clones library. Clustering analysis revealed a 3504 unique including 739 contigs (assembled 2594 ESTs) 2765 singletons. BLAST against GenBank nr database resulted 1287 significant hits...
MicroRNAs are very small endogenous RNA molecules that play a crucial role in an array of biological processes, including regulation skin morphogenesis. The microRNA let-7b is thought to modulate animal hair growth, by binding target genes encode growth factors. Fibroblast factor 5 (FGF5) has been previously reported be involved the initiation catagen phase growth. In this study, we combined previous reports with bioinformatic analysis techniques identify and validate FGF5 and, using...