A5055551634- Oral microbiology and periodontitis research
- Mesenchymal stem cell research
- Dental Radiography and Imaging
- Periodontal Regeneration and Treatments
- Extracellular vesicles in disease
- dental development and anomalies
- Cancer-related molecular mechanisms research
- Immune Response and Inflammation
- Immune cells in cancer
- RNA Interference and Gene Delivery
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Bone Tissue Engineering Materials
- RNA modifications and cancer
- Endodontics and Root Canal Treatments
- Dental Trauma and Treatments
- Dental materials and restorations
- Nanoplatforms for cancer theranostics
- Forensic Anthropology and Bioarchaeology Studies
- Autophagy in Disease and Therapy
- Oral Health Pathology and Treatment
- MicroRNA in disease regulation
- Tissue Engineering and Regenerative Medicine
- Bone Metabolism and Diseases
- Cellular Mechanics and Interactions
- Adenosine and Purinergic Signaling
Air Force Medical University
2016-2025
Stomatology Hospital
2017
Abstract Background Periodontitis, which progressively destroys tooth-supporting structures, is one of the most widespread infectious diseases and leading cause tooth loss in adults. Evidence from preclinical trials small-scale pilot clinical studies indicates that stem cells derived periodontal ligament tissues are a promising therapy for regeneration lost/damaged tissue. This study assessed safety feasibility using autologous (PDLSCs) as an adjuvant to grafting materials guided tissue...
Exosomes (EXs) shed by mesenchymal stem cells (MSCs) are potent therapeutic agents that promote wound healing and regeneration, but when used alone in vivo, their potency is diminished rapid clearance bioactivity loss. Inspired the biotin-avidin interaction, we developed a simple yet versatile method for immobilization of MSC-derived EXs (MSC-EXs) into hydrogels achieved sustained release regenerative purposes. First, biotin-modified gelatin methacryloyl (Bio-GelMA) was fabricated grafting...
Periodontal ligament stem cells (PDLSCs) are a key cell type for restoring/regenerating lost/damaged periodontal tissues, including alveolar bone, and root cementum, the latter of which is important regaining tooth function. However, PDLSCs residing in an inflammatory environment generally exhibit compromised functions, as demonstrated by impaired ability to differentiate into cementoblasts, responsible regrowing cementum. This study investigated role mitochondrial function downstream long...
Abstract The viscoelasticity of mechanically sensitive tissues such as periodontal ligaments (PDLs) is key in maintaining mechanical homeostasis. Unfortunately, PDLs easily lose (e.g., stress relaxation) during periodontitis or dental trauma, which disrupt cell–extracellular matrix (ECM) interactions and accelerates tissue damage. Here, Pluronic F127 diacrylate (F127DA) hydrogels with PDL‐matched relaxation rates high elastic moduli are developed. hydrogel modulated without chemical...
Background Different phenotypes of macrophages (M0, M1 and M2 Mφs) have been demonstrated to play distinct roles in regulating mesenchymal stem cells various vitro vivo systems. Our previous study also found that cell-conditioned medium (CM) derived from Mφs supported the proliferation adipogenic differentiation bone marrow (BMMSCs), whereas CM either M0 or showed an enhanced effect on cell osteogenic differentiation. However, underlying mechanism remains incompletely elucidated. Exosomes,...
Although dental pulp stem cells (DPSCs) isolated from periodontally compromised teeth (P-DPSCs) have been demonstrated to retain pluripotency and regenerative potential, their use as therapeutics remains largely unexplored. In this study, we investigated the proangiogenic effects of extracellular vesicles (EVs) secreted by P-DPSCs using in vitro vivo testing models.Patient-matched DPSCs derived healthy (H-DPSCs) were used control for P-DPSCs. Conditioned media (CMs) H-DPSCs (H-CM P-CM), CMs...
The positive regulation of bone-forming osteoblast activity and the negative feedback osteoclastic are equally important in strategies to achieve successful alveolar bone regeneration. Here, a molybdenum (Mo)-containing bioactive glass ceramic scaffold with solid-strut-packed structures (Mo-scaffold) was printed, its ability regulate pro-osteogenic anti-osteoclastogenic cellular responses evaluated vitro vivo. We found that extracts derived from Mo-scaffold (Mo-extracts) strongly stimulated...
Although macrophage (Mφ) polarization has been demonstrated to play crucial roles in cellular osteogenesis across the cascade of events periodontal regeneration, how polarized Mφ phenotypes influence cementoblastic differentiation ligament stem cells (PDLSCs) remains unknown. In present study, human monocyte leukemic (THP-1) were induced into M0, M1, and M2 subsets, influences these Mφs on PDLSCs assessed both conditioned medium-based Transwell-based coculture systems. Furthermore, potential...
Although cellular therapy has been proposed for inflammation-related disorders such as periodontitis decades, clinical application unsuccessful. One explanation these disappointing results is that the functions of stem cells are substantially compromised when they transplanted into an inflammatory in vivo milieu. Considering previous finding P2X7 receptor (P2X7R) gene modification able to reverse inflammation-mediated impairment periodontal ligament (PDLSCs), we further hypothesized...
Stem cells that have undergone long-term ex vivo expansion are most likely functionally compromised (namely cellular senescence) in terms of their stem cell properties and therapeutic potential. Due to its ability attenuate senescence, melatonin (MLT) has been proposed as an adjuvant protocols, but the mechanism underlying MLT-induced rejuvenation remains largely unknown.Human periodontal ligament (PDLSCs) were isolated cultured for up 15 passages, from passages 2, 7, (P2, P7, P15) used...
Periodontitis is an intractable chronic inflammatory disease characterized by excessive proinflammatory factors production and reactive oxygen species (ROS) accumulation. Nanodrugs have made significant contributions in the biomedical field but fall short as periodontitis therapeutics because of their singular effects, low retention rates, poor specificity, insufficient biocompatibility. Herein, we report a functionalized periodontal ligament stem cell (PDLSC) membrane-camouflaged MnO2...
This study focused on whether the effectiveness of nonsurgical periodontal therapy (NSPT) second molars (M2s) varies with status adjacent nonimpacted third (N-M3s). In this retrospective cohort study, 776 regions from 268 patients were evaluated. Based N-M3 status, quadrants M2s categorized into three groups: group (present N-M3), A-M3 (absent M3), and R-M3 (removed N-M3). The probing depth reduction (ΔPD) percentage reversed deep pockets (PD5+ [probing ≥5 mm] turned to PD5-) after NSPT...
Diabetes mellitus is an established risk factor for periodontal disease that can aggravate the severity of inflammation and accelerate destruction. The chronic high glucose condition a hallmark diabetes-related pathogenesis, has been demonstrated to impair osteogenic differentiation ligament stem cells (PDLSCs), leading delayed recovery defects in diabetic patients. Reactive oxygen species (ROS) are small molecules influence cell fate determination direction differentiation. Although...
Background: Although removal of impacted third molars (I‐M3s) is common in dental clinics, the decision to retain or remove asymptomatic non‐impacted (N‐M3s) presents a significant challenge. This study investigates influence N‐M3s on pathologies adjacent second (A‐M2s). Methods: Clinical status M3s was evaluated, and presence distal caries, external root resorption (ERR), alveolar bone loss (ABL) A‐M2s assessed by orthopantomograms (OPGs). Prevalence A‐M2 evaluated association between...
This study aimed to investigate the relationship between inflammation-related T-helper cell polarization and receptor activator for nuclear factor-κB ligand (RANKL)/osteoprotegerin (OPG) ratio, which is associated with bone resorption or remodeling of chronic periodontitis patients. Gingival crevicular fluid (GCF) gingival tissues were obtained from periodontally healthy individuals (PH group) patients (CP group). The GCF levels IFN-γ, IL-4, IL-17, IL-10 linked toward Th1, Th2, Th17, Treg...
Although the immunomodulatory properties of calcitriol in bone metabolism have been documented for decades, its therapeutic role management periodontitis remains largely unexplored. In this study, we hypothesized that suppresses lipopolysaccharide (LPS)-induced alveolar loss by regulating T helper (Th) cell subset polarization.To test hypothesis, determined effect intervention on development LPS-induced rats terms (micro-CT analysis), local inflammatory infiltration levels, number...
Although obesity has been proposed as a risk factor for periodontitis, the influence of excessive fat accumulation on development periodontitis and periodontal recovery from disease remains largely unknown. This study investigated cellular response ligament stem cells (PDLSCs) to elevated levels specific fatty acid, namely, palmitic acid (PA). The mechanism by which PA exposure compromises osteogenic potential was also explored. It found that PDLSCs abundant led decreased cell...